BBa_K1932001 1 BBa_K1932001 orf1 and orf2 from Bifidobacterium 2016-10-13T11:00:00Z 2016-10-19T09:39:39Z the plasmid of PMB1 from Bifidobacterium Part BBa_1932001 was originally cloned from the plasmid of Bifidobacterium that contains a sequence of ori and two sequences of orf.（The plasmid had a G + C content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon.）This sequence is essential for the shuttling of the plasmid from E.coli to Bifidobacterium. false false _2399_ 31111 31111 9 false （1）The restriction enzyme cutting site were identified. （2）The sequences of ori and orf were confirmed. false Jin Liu annotation2516407 1 orf1 range2516407 1 1253 1570 annotation2516408 1 orf2 range2516408 1 339 1259 BBa_K1932001_sequence 1 ctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatgga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z