BBa_K1932004 1 BBa_K1932004 TAT+apoptin 2016-10-13T11:00:00Z 2016-10-16T02:06:44Z HIV-1 and CAV TAT-Apoptin is a fusion protein made up of the transduction domain of HIV-1 and apoptin. Since apoptin only functions inside the cells, the peptide TAT is added to direct the transfer of the apoptin into the cell. Once transferred into the tumor cells, apoptin can induce the apoptosis of them potently. false false _2399_ 31111 31111 9 false (1) The restriction enzyme cutting site was identified. (2) The appropriate sequences of TAT and apoptin were selected. false Jin Liu annotation2512808 1 apoptin range2512808 1 67 432 annotation2512807 1 linker range2512807 1 37 66 annotation2512806 1 TAT range2512806 1 1 36 BBa_K1932005 1 BBa_K1932005 This device is made up of BBa_K1932000;BBa_K1932001;BBa_K1932004 2016-10-13T11:00:00Z 2016-10-16T02:00:05Z Gene pool The device is designed to replicate stably and express robustly of the TAT-apoptin. Among the subparts, BBa_K1932000 is added to up-regulate the expression of exogenous protein in Bifidobacterium with the presence of a strong promoter. BBa_K1932001 is constructed into the device to increase the stability of the device in Bifidobacterium. BBa_K1932004 encodes TAT-apoptin, which acts as the effector protein to kill the cancer cells. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting sites were identified. &#65288;2&#65289;The order of the parts was confirmed. false Jin Liu component2512767 1 BBa_K1932000 component2512768 1 BBa_K1932001 component2512769 1 BBa_K1932004 annotation2512769 1 BBa_K1932004 range2512769 1 1873 2238 annotation2512768 1 BBa_K1932001 range2512768 1 125 1866 annotation2512767 1 BBa_K1932000 range2512767 1 1 116 BBa_K1932001 1 BBa_K1932001 orf1 and orf2 from Bifidobacterium 2016-10-13T11:00:00Z 2016-10-19T09:39:39Z the plasmid of PMB1 from Bifidobacterium Part BBa_1932001 was originally cloned from the plasmid of Bifidobacterium that contains a sequence of ori and two sequences of orf.&#65288;The plasmid had a G + C content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon.&#65289;This sequence is essential for the shuttling of the plasmid from E.coli to Bifidobacterium. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting site were identified. &#65288;2&#65289;The sequences of ori and orf were confirmed. false Jin Liu annotation2516408 1 orf2 range2516408 1 339 1259 annotation2516407 1 orf1 range2516407 1 1253 1570 BBa_K1932000 1 BBa_K1932000 A promoter and an RBS of the B.<i>longum</i> <i>hup</i> gene. 2016-10-13T11:00:00Z 2016-10-19T03:09:28Z the genomic DNA of Bifidobacterium longum BBa_1932000 contains a part of the hup gene. This gene was cloned from Bifidobacterium to express the HU family protein, HB1, and it consisted a promoter, an RBS, a structural gene and a terminator. Our part was constructed to up-regulate the expression of the protein in Bifidobacterium. false false _2399_ 31111 31111 9 false 1&#12289;Restriction enzyme cutting sites were identified. 2&#12289;The sequence of strong promoter was assured false Jin Liu annotation2512761 1 Hup range2512761 1 1 116 BBa_K1932001_sequence 1 ctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatgga BBa_K1932000_sequence 1 agatgtgaaaacccttataaaacgcgggttttcgcagaaacatgcgctagtatcattgatgacaacatggactaagcaaaagtgcttgtcccctgacccaagaaggatgctttatg BBa_K1932004_sequence 1 atgtatggccgcaaaaagcgtcgccagcgtcgccgtggcggtggcggtagcggcggtggcggttctatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaa BBa_K1932005_sequence 1 agatgtgaaaacccttataaaacgcgggttttcgcagaaacatgcgctagtatcattgatgacaacatggactaagcaaaagtgcttgtcccctgacccaagaaggatgctttatgtactagagctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatggatactagatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z