BBa_K1932002 1 BBa_K1932002 SEC2 signal peptides 2016-10-13T11:00:00Z 2016-10-19T02:13:44Z sequenced genome of B. breve UCC2003 Sec2 is predicted to contain a classical SP with a single transmembrane region. The portion of this protein fused to the nuclease comprises the first 77 amino acids of a putative 602-amino-acid protein. This protein is significantly similar to the permease component of an ABC-type transport system, and clear homologues of the gene are found in B. longum NCC2705 (accession no. NP_695398) and DJO10A (ZP_00121339) false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting sites were excluded. &#65288;2&#65289;The protein region that directs the export was confirmed. false Jin Liu annotation2512805 1 sec2 signal peptide range2512805 1 1 134 BBa_K1932001 1 BBa_K1932001 orf1 and orf2 from Bifidobacterium 2016-10-13T11:00:00Z 2016-10-19T09:39:39Z the plasmid of PMB1 from Bifidobacterium Part BBa_1932001 was originally cloned from the plasmid of Bifidobacterium that contains a sequence of ori and two sequences of orf.&#65288;The plasmid had a G + C content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon.&#65289;This sequence is essential for the shuttling of the plasmid from E.coli to Bifidobacterium. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting site were identified. &#65288;2&#65289;The sequences of ori and orf were confirmed. false Jin Liu annotation2516407 1 orf1 range2516407 1 1253 1570 annotation2516408 1 orf2 range2516408 1 339 1259 BBa_K1932000 1 BBa_K1932000 A promoter and an RBS of the B.<i>longum</i> <i>hup</i> gene. 2016-10-13T11:00:00Z 2016-10-19T03:09:28Z the genomic DNA of Bifidobacterium longum BBa_1932000 contains a part of the hup gene. This gene was cloned from Bifidobacterium to express the HU family protein, HB1, and it consisted a promoter, an RBS, a structural gene and a terminator. Our part was constructed to up-regulate the expression of the protein in Bifidobacterium. false false _2399_ 31111 31111 9 false 1&#12289;Restriction enzyme cutting sites were identified. 2&#12289;The sequence of strong promoter was assured false Jin Liu annotation2512761 1 Hup range2512761 1 1 116 BBa_K1932004 1 BBa_K1932004 TAT+apoptin 2016-10-13T11:00:00Z 2016-10-16T02:06:44Z HIV-1 and CAV TAT-Apoptin is a fusion protein made up of the transduction domain of HIV-1 and apoptin. Since apoptin only functions inside the cells, the peptide TAT is added to direct the transfer of the apoptin into the cell. Once transferred into the tumor cells, apoptin can induce the apoptosis of them potently. false false _2399_ 31111 31111 9 false (1) The restriction enzyme cutting site was identified. (2) The appropriate sequences of TAT and apoptin were selected. false Jin Liu annotation2512807 1 linker range2512807 1 37 66 annotation2512808 1 apoptin range2512808 1 67 432 annotation2512806 1 TAT range2512806 1 1 36 BBa_K1932006 1 BBa_K1932006 This device is constructed to express TAT-apoptin fused with sec2. 2016-10-13T11:00:00Z 2016-10-16T02:01:37Z gene pool This device was cloned to express the fusion protein, TAT-apoptin and the sec2 peptide coding sequence was included to increase the secretion of the protein. Among the subparts, BBa_1932000 is a strong promoter to regulate the expression of exogenous protein in Bifidobacterium. BBa_1932001 is included to enhance the stability of the device in Bifidobacterium. BBa_193202 is the sequence of the signal peptide, sec2, that can direct the export of the protein from Bididobacterium. BBa_1932004 encodes the TAT-apoptin fusion protein, which can induce the apoptosis specifically in the cancer cells. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting site were identified. &#65288;2&#65289;The order of the parts are confirm false Jin Liu component2512772 1 BBa_K1932001 component2512773 1 BBa_K1932002 component2512771 1 BBa_K1932000 component2512774 1 BBa_K1932004 annotation2512771 1 BBa_K1932000 range2512771 1 1 116 annotation2512774 1 BBa_K1932004 range2512774 1 2015 2380 annotation2512773 1 BBa_K1932002 range2512773 1 1875 2008 annotation2512772 1 BBa_K1932001 range2512772 1 125 1866 BBa_K1932001_sequence 1 ctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatgga BBa_K1932006_sequence 1 agatgtgaaaacccttataaaacgcgggttttcgcagaaacatgcgctagtatcattgatgacaacatggactaagcaaaagtgcttgtcccctgacccaagaaggatgctttatgtactagagctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatggatactagagtggaacatatgaagatgttccggcgcctatcctccgttgtcgttattgtgctgctgatgccgctcatactggtggccatgccggttcccgccgcgcaagccgaccagctgcccaaccccgactgggtggcattgtactagatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaa BBa_K1932002_sequence 1 atggaacatatgaagatgttccggcgcctatcctccgttgtcgttattgtgctgctgatgccgctcatactggtggccatgccggttcccgccgcgcaagccgaccagctgcccaaccccgactgggtggcattg BBa_K1932000_sequence 1 agatgtgaaaacccttataaaacgcgggttttcgcagaaacatgcgctagtatcattgatgacaacatggactaagcaaaagtgcttgtcccctgacccaagaaggatgctttatg BBa_K1932004_sequence 1 atgtatggccgcaaaaagcgtcgccagcgtcgccgtggcggtggcggtagcggcggtggcggttctatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z