BBa_K1932004 1 BBa_K1932004 TAT+apoptin 2016-10-13T11:00:00Z 2016-10-16T02:06:44Z HIV-1 and CAV TAT-Apoptin is a fusion protein made up of the transduction domain of HIV-1 and apoptin. Since apoptin only functions inside the cells, the peptide TAT is added to direct the transfer of the apoptin into the cell. Once transferred into the tumor cells, apoptin can induce the apoptosis of them potently. false false _2399_ 31111 31111 9 false (1) The restriction enzyme cutting site was identified. (2) The appropriate sequences of TAT and apoptin were selected. false Jin Liu annotation2512808 1 apoptin range2512808 1 67 432 annotation2512807 1 linker range2512807 1 37 66 annotation2512806 1 TAT range2512806 1 1 36 BBa_K1932001 1 BBa_K1932001 orf1 and orf2 from Bifidobacterium 2016-10-13T11:00:00Z 2016-10-19T09:39:39Z the plasmid of PMB1 from Bifidobacterium Part BBa_1932001 was originally cloned from the plasmid of Bifidobacterium that contains a sequence of ori and two sequences of orf.&#65288;The plasmid had a G + C content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon.&#65289;This sequence is essential for the shuttling of the plasmid from E.coli to Bifidobacterium. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting site were identified. &#65288;2&#65289;The sequences of ori and orf were confirmed. false Jin Liu annotation2516408 1 orf2 range2516408 1 339 1259 annotation2516407 1 orf1 range2516407 1 1253 1570 BBa_K1932007 1 BBa_K1932007 This device is constructed for the expression of TAT-apoptin fused with tmp1. 2016-10-13T11:00:00Z 2016-10-16T02:02:25Z gene pool This device is constructed to express the fusion protein, TAT-apoptin and the region of tmp1 is included to increase the secretion quantity of the protein. Among the subparts, BBa_1932000 is added as a promoter to regulate the expression of exogenous protein in Bifidobacterium with. BBa_1932001 is included into the device to increase the stability of the device in Bifidobacterium. BBa_193203 encodes the signal peptide, tmp1, which can direct the export of the protein from Bididobacterium. BBa_1932004 encodes TAT-apoptin, which acts as the effector protein to kill the cancer cells. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting sites were identified. &#65288;2&#65289;The order of the parts was confirm false Jin Liu component2512776 1 BBa_K1932000 component2512777 1 BBa_K1932001 component2512779 1 BBa_K1932004 component2512778 1 BBa_K1932003 annotation2512776 1 BBa_K1932000 range2512776 1 1 116 annotation2512777 1 BBa_K1932001 range2512777 1 125 1866 annotation2512779 1 BBa_K1932004 range2512779 1 2078 2443 annotation2512778 1 BBa_K1932003 range2512778 1 1875 2071 BBa_K1932000 1 BBa_K1932000 A promoter and an RBS of the B.<i>longum</i> <i>hup</i> gene. 2016-10-13T11:00:00Z 2016-10-19T03:09:28Z the genomic DNA of Bifidobacterium longum BBa_1932000 contains a part of the hup gene. This gene was cloned from Bifidobacterium to express the HU family protein, HB1, and it consisted a promoter, an RBS, a structural gene and a terminator. Our part was constructed to up-regulate the expression of the protein in Bifidobacterium. false false _2399_ 31111 31111 9 false 1&#12289;Restriction enzyme cutting sites were identified. 2&#12289;The sequence of strong promoter was assured false Jin Liu annotation2512761 1 Hup range2512761 1 1 116 BBa_K1932003 1 BBa_K1932003 TMP1 signal peptides, hypothetical protein 2016-10-13T11:00:00Z 2016-10-16T03:56:14Z sequenced genome of B. breve UCC2003 TMP1 is a polypeptide containing at least one predicted transmembrane domain were identified as active &#916;SPNuc fusions and were designated Tmp (for putative transmembrane protein).The fused portion of Tmp1 corresponds to the N-terminal region of a hypothetical protein and is predicted to have a C-out topology, which is expected for transmembrane proteins (Nuc activity of a fusion indicates that the &#916;SPNuc domain is exported). Proteins similar to Tmp1 have been found only in B. longum NCC2705 (accession no. NP_696268) and DJO10A (ZP_00120937), although no function has been assigned to these proteins. false false _2399_ 31111 31111 9 false &#65288;1&#65289;The restriction enzyme cutting sites were excluded. &#65288;2&#65289;The protein region that directs the export was confirmed. false Jin Liu annotation2512804 1 TMP1 signal peptide range2512804 1 1 198 BBa_K1932001_sequence 1 ctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatgga BBa_K1932000_sequence 1 agatgtgaaaacccttataaaacgcgggttttcgcagaaacatgcgctagtatcattgatgacaacatggactaagcaaaagtgcttgtcccctgacccaagaaggatgctttatg BBa_K1932004_sequence 1 atgtatggccgcaaaaagcgtcgccagcgtcgccgtggcggtggcggtagcggcggtggcggttctatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaa BBa_K1932003_sequence 1 atgacactgatggcaggccgggaaagaaggtcgatgatgactggtgcacaggcttctcactgtggttccgtatccgcaatttcactgggactgcctgtttccacggcgattccggaggccaagggcatattgccgaaggctctgttcgtgggcaaagcgccgatttccggcaagcttaagcagcggtttgtgaatgag BBa_K1932007_sequence 1 agatgtgaaaacccttataaaacgcgggttttcgcagaaacatgcgctagtatcattgatgacaacatggactaagcaaaagtgcttgtcccctgacccaagaaggatgctttatgtactagagctgaccgcacgcgaacgccatccggttcatcgtcgcccactccgtctcggtcaaccgcaggcaaatcctggtcttcttccccaatggcgccgcgggcctcagcctgcggaacgcgcagcggacgccgacggctcagacggctcagaaacgtccgtgagtggcctccacgcggccgaacaggtcagggaggctcgcgcatacgtgagcggcgtggagaagcggctgaaggccgtccagcggcttttcgtgcaggatgtgctgggctgggttcagccgacgcttcgctgggctgaaatatctgacttggttcccgcgtatttgttcactgtacaaatacgatgtatgctgtagccatgtccgatgagtattcgcagccgacgcttgagctgtcgcgcacgttcgaaggctggtggctgcccgaacgcccgctgtgctgcgacgacgactactcccggctgcaccgcaggagccgcgccgacgcgctcaaatgcaagcacatcgaggcgaaccccgccgcgctggtgaacacgatcgtggtggacatcgacgacgcgaacgccaaggcgatggccctgtgggagcacgagggcatgcggccgaactggatcgcggagaacccggccaacgggcacgctcacgcgggctgggtgctcacctttccggtgcccagaaccgatctggcgcgtctcaagccgttgaagctcctgcacgccaccacggagggactgcgccgctcctgcgacggggacatgggctattcgggacttctgatgaagaaccccgagcatccggcgtgggcgtcggacatcatcgagtgggacacctacgacctggaacagctcgtgcagtcgctccaggaacacggggacatgccgcccgtcagctggaagcgcaccaagcgcgcccgcacgcaggggctgggacgcaactgcacgctcttcgacaaggcccgcacgctcgcctaccgctacgttgcggcggctgccgaccgttcggaggccagcagcgaggcattgcgcctatacgtgcgtcgcacctgccacgaactcaacgtctcgctgttccccgatccgctgcacgcgcgtgaggtcgaggacatcgccaagagcatccacaaatggatcgtcacccgcagccgcatgtggcgcgacggtgccattgccaacgcagccacattcatcgccatccaatccgcacgaggacacaaacacggtgagaacaaatatcagcaggtcatgaaggaggcactggaatggtaaggacgactttgaggaagaagcgcccggtgtctgcacgtgaattagctgaagcatacggcgtctccacgcgcaccattcagagctgggtggcaatgaagcgcgaggattggattgatgaacaagccgctatgcgcgaagcagtccgctcatatcacgatgacgagggccatacatggccgcagaccgccgagcatttcaacatgagccagggtgccgtgcgtcaacgctgctacagggctcgcaaggagcgcgaggacgaggcggcggagaaatcgaagcatctacccggcgagattccactgttcgactgacgctaaacgttgtcccaaacgcgaacgcagcacctccctcgccttgcggctttttcctcttccatcggccttcggcactcgggttgttgctccagcgccgcagggcgcgggaggctgcgggctcggattgtgtatacaatccgtctagcttgcttaccttcgatttgatggatactagagtgacactgatggcaggccgggaaagaaggtcgatgatgactggtgcacaggcttctcactgtggttccgtatccgcaatttcactgggactgcctgtttccacggcgattccggaggccaagggcatattgccgaaggctctgttcgtgggcaaagcgccgatttccggcaagcttaagcagcggtttgtgaatgagtactagatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z