LOCUS BBa_K193207 1126 bp DNA linear 22-Nov-2024 DEFINITION TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein ACCESSION BBa_K193207 VERSION BBa_K193207.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 RBS 46..60 /label=RBS-1\Strong RBS 53..57 /label=RBS misc_feature 46..60 /label=BBa_B0030 RBS 46..60 /label=BBa_B0030 CDS 69..1126 /label=BBa_K193203 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagattaa agaggagaaa 61 tactagagat atacatatga gtaaaggaga agaacttttc actggagttg tcccaattct 121 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg 181 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt 241 tccatggcca acacttgtca ctactttgac ttatggtgtt caatgctttt cccgttatcc 301 ggatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga 361 acgcactata tttttcaaag atgacgggaa ctacaagacg cgtgctgaag tcaagtttga 421 aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt gattttaaag aagatggaaa 481 cattctcgga cacaaactcg agtacaacta taactcacac aatgtataca tcatggcaga 541 caaacaaaag aatggaatca aagttaactt caaaattcgc cacaacattg aagatggatc 601 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 661 accagacaac cattacctgt cgacacaatc tgccctttcg aaagatccca acgaaaagcg 721 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga 781 gctctacaaa tatcgatccc agtgcaccgg tggtgctgac tgcaccagct gcaccgctgc 841 ttgcaccggt tgcggtaact gcccgaacgc tgttacttgc actaacagcc agcactgcgt 901 taaagctacc acctgcaccg gtagcaccga ctgcaacacc gctgttacct gcactaactc 961 taaagactgc ttcgaagctc agacctgcac cgactctacc aactgctaca aagctaccgc 1021 ttgcaccaac tctaccggct gcccgggtca cgatcgatat gaattccaac tgagcgccgc 1081 tagcaagctt gcggccgcac tcgagcacca ccaccaccac cactaa //