LOCUS       BBa_K193207             1126 bp    DNA     linear       06-May-2024
DEFINITION  TmTHP with promotor for constituitive expression and with EGFP for 
            fusion of Anti-Freeze Protein
ACCESSION   BBa_K193207
VERSION     BBa_K193207.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     RBS             46..60
                     /label=RBS-1\Strong
     RBS             53..57
                     /label=RBS
     misc_feature    46..60
                     /label=BBa_B0030
     RBS             46..60
                     /label=BBa_B0030
     CDS             69..1126
                     /label=BBa_K193203
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagattaa agaggagaaa
       61 tactagagat atacatatga gtaaaggaga agaacttttc actggagttg tcccaattct
      121 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      181 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      241 tccatggcca acacttgtca ctactttgac ttatggtgtt caatgctttt cccgttatcc
      301 ggatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      361 acgcactata tttttcaaag atgacgggaa ctacaagacg cgtgctgaag tcaagtttga
      421 aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt gattttaaag aagatggaaa
      481 cattctcgga cacaaactcg agtacaacta taactcacac aatgtataca tcatggcaga
      541 caaacaaaag aatggaatca aagttaactt caaaattcgc cacaacattg aagatggatc
      601 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      661 accagacaac cattacctgt cgacacaatc tgccctttcg aaagatccca acgaaaagcg
      721 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
      781 gctctacaaa tatcgatccc agtgcaccgg tggtgctgac tgcaccagct gcaccgctgc
      841 ttgcaccggt tgcggtaact gcccgaacgc tgttacttgc actaacagcc agcactgcgt
      901 taaagctacc acctgcaccg gtagcaccga ctgcaacacc gctgttacct gcactaactc
      961 taaagactgc ttcgaagctc agacctgcac cgactctacc aactgctaca aagctaccgc
     1021 ttgcaccaac tctaccggct gcccgggtca cgatcgatat gaattccaac tgagcgccgc
     1081 tagcaagctt gcggccgcac tcgagcacca ccaccaccac cactaa
//