BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K193207
1
pLacIQ+eGF
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein
2009-10-13T11:00:00Z
2015-05-08T01:11:15Z
We used I14032 and K193205.
TmTHP with promotor for constituitive expression and with eGFP for fusion of Anti-Freeze Protein.
false
false
_288_
0
4081
9
Not in stock
false
We used biobrick standard assembly.
false
Nao Nakatani
component2040911
1
BBa_I14032
component2040913
1
BBa_B0030
component2040915
1
BBa_K193203
annotation2040913
1
BBa_B0030
range2040913
1
46
60
annotation2040915
1
BBa_K193203
range2040915
1
69
1126
annotation2040911
1
BBa_I14032
range2040911
1
1
37
BBa_I14032
1
P(Lac) IQ
promoter P(Lac) IQ
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pMAL-p2X
Released HQ 2013
Constitutive Promoter, High Transcription
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1028344
1
-10
range1028344
1
26
31
annotation1028342
1
P(Lac) IQ
range1028342
1
1
37
annotation1028343
1
-35
range1028343
1
3
8
BBa_K193203
1
eGFP+TmTHP
TmTHP with EGFP for fusion of Antifreeze protein
2009-10-13T11:00:00Z
2015-05-08T01:11:15Z
The gene sequence was obtained from clonetech.
TmTHP with eGFP for fusion of Antifreeze protein.
false
false
_288_
0
4081
9
Not in stock
false
false
Nao Nakatani
BBa_K193203_sequence
1
atatacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatttttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatatcgatcccagtgcaccggtggtgctgactgcaccagctgcaccgctgcttgcaccggttgcggtaactgcccgaacgctgttacttgcactaacagccagcactgcgttaaagctaccacctgcaccggtagcaccgactgcaacaccgctgttacctgcactaactctaaagactgcttcgaagctcagacctgcaccgactctaccaactgctacaaagctaccgcttgcaccaactctaccggctgcccgggtcacgatcgatatgaattccaactgagcgccgctagcaagcttgcggccgcactcgagcaccaccaccaccaccactaa
BBa_I14032_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K193207_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagagatatacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatttttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatatcgatcccagtgcaccggtggtgctgactgcaccagctgcaccgctgcttgcaccggttgcggtaactgcccgaacgctgttacttgcactaacagccagcactgcgttaaagctaccacctgcaccggtagcaccgactgcaacaccgctgttacctgcactaactctaaagactgcttcgaagctcagacctgcaccgactctaccaactgctacaaagctaccgcttgcaccaactctaccggctgcccgggtcacgatcgatatgaattccaactgagcgccgctagcaagcttgcggccgcactcgagcaccaccaccaccaccactaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z