LOCUS       BBa_K193209             1167 bp    DNA     linear       25-Nov-2024
DEFINITION  Fusion protein (TmTHP - EGFP) as Anti-Freeze Protein regulated by 
            LacI
ACCESSION   BBa_K193209
VERSION     BBa_K193209.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=T7
     promoter        1..78
                     /label=BBa_K193206
     RBS             87..101
                     /label=RBS-1\Strong
     RBS             94..98
                     /label=RBS
     misc_feature    87..101
                     /label=BBa_B0030
     RBS             87..101
                     /label=BBa_B0030
     CDS             110..1167
                     /label=BBa_K193203
ORIGIN
        1 taatacgact cactataggg agaactagac cgatcctgtc ggagatctga aataattttg
       61 tttaacttta agaaggagta ctagagatta aagaggagaa atactagaga tatacatatg
      121 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      181 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      241 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      301 actactttga cttatggtgt tcaatgcttt tcccgttatc cggatcatat gaaacggcat
      361 gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat atttttcaaa
      421 gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      481 cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg acacaaactc
      541 gagtacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      601 aaagttaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact agcagaccat
      661 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      721 tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt
      781 gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa atatcgatcc
      841 cagtgcaccg gtggtgctga ctgcaccagc tgcaccgctg cttgcaccgg ttgcggtaac
      901 tgcccgaacg ctgttacttg cactaacagc cagcactgcg ttaaagctac cacctgcacc
      961 ggtagcaccg actgcaacac cgctgttacc tgcactaact ctaaagactg cttcgaagct
     1021 cagacctgca ccgactctac caactgctac aaagctaccg cttgcaccaa ctctaccggc
     1081 tgcccgggtc acgatcgata tgaattccaa ctgagcgccg ctagcaagct tgcggccgca
     1141 ctcgagcacc accaccacca ccactaa
//