BBa_K193206
1
BBa_K193206
T7 promotor with lac operator
2009-10-13T11:00:00Z
2015-05-08T01:11:15Z
T7 is from I719005 and lac operator is from operon.
T7 promotor regulated by LacI
false
false
_288_
0
4081
9
Not in stock
false
false
Nao Nakatani
annotation2040907
1
T7
range2040907
1
1
23
BBa_K193203
1
eGFP+TmTHP
TmTHP with EGFP for fusion of Antifreeze protein
2009-10-13T11:00:00Z
2015-05-08T01:11:15Z
The gene sequence was obtained from clonetech.
TmTHP with eGFP for fusion of Antifreeze protein.
false
false
_288_
0
4081
9
Not in stock
false
false
Nao Nakatani
BBa_K193209
1
AntiFreeze
Fusion protein (TmTHP - EGFP) as Anti-Freeze Protein regulated by LacI
2009-10-13T11:00:00Z
2015-05-08T01:11:15Z
We used K193206 and K193205.
Fusion protein (TmTHP - eGFP) as Anti-Freeze Protein regulated by LacI.
false
false
_288_
0
4081
9
It's complicated
false
We used biobrick standard assembly.
false
Nao Nakatani
component2040925
1
BBa_B0030
component2040927
1
BBa_K193203
component2040923
1
BBa_K193206
annotation2040923
1
BBa_K193206
range2040923
1
1
78
annotation2040927
1
BBa_K193203
range2040927
1
110
1167
annotation2040925
1
BBa_B0030
range2040925
1
87
101
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K193209_sequence
1
taatacgactcactatagggagaactagaccgatcctgtcggagatctgaaataattttgtttaactttaagaaggagtactagagattaaagaggagaaatactagagatatacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatttttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatatcgatcccagtgcaccggtggtgctgactgcaccagctgcaccgctgcttgcaccggttgcggtaactgcccgaacgctgttacttgcactaacagccagcactgcgttaaagctaccacctgcaccggtagcaccgactgcaacaccgctgttacctgcactaactctaaagactgcttcgaagctcagacctgcaccgactctaccaactgctacaaagctaccgcttgcaccaactctaccggctgcccgggtcacgatcgatatgaattccaactgagcgccgctagcaagcttgcggccgcactcgagcaccaccaccaccaccactaa
BBa_K193203_sequence
1
atatacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatttttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatatcgatcccagtgcaccggtggtgctgactgcaccagctgcaccgctgcttgcaccggttgcggtaactgcccgaacgctgttacttgcactaacagccagcactgcgttaaagctaccacctgcaccggtagcaccgactgcaacaccgctgttacctgcactaactctaaagactgcttcgaagctcagacctgcaccgactctaccaactgctacaaagctaccgcttgcaccaactctaccggctgcccgggtcacgatcgatatgaattccaactgagcgccgctagcaagcttgcggccgcactcgagcaccaccaccaccaccactaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K193206_sequence
1
taatacgactcactatagggagaactagaccgatcctgtcggagatctgaaataattttgtttaactttaagaaggag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z