BBa_K1933000
1
BclA-His
N-terminal domain of BclA protein fused to 6xHis tag
2016-10-09T11:00:00Z
2016-10-17T12:19:04Z
NCBI accession number CAD56880
NCBI accession number CAD56878
NCBI accession number CAD56869
NCBI accession number CAD56870
This part codes N-terminal domain of BclA protein which expressed on the cell surface connected by a 6??His tag to be easily identified by Western blotting.[1] Surface displayed passengers retain enzyme activity even when it is linked to BclA with 6?? His tag.
false
false
_2400_
23701
29998
9
false
optimized to expression in E. coli K-12 strain
false
Tomoki Uchino
annotation2490250
1
6xHis tag
range2490250
1
67
84
annotation2490249
1
BclA
range2490249
1
4
66
annotation2490248
1
start codon
range2490248
1
1
3
BBa_K1933000_sequence
1
atggcgttcgacccgaacctggttggtccgaccctgccgccgatcccgccgttcaccctgccgacccatcatcatcatcatcat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z