BBa_K1933001 1 INP-His INPNC fused to 6xHis tag 2016-10-09T11:00:00Z 2016-10-17T12:46:05Z INPNC derived from BBa_K811005. INPNC codes for N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6??His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passenger domain. INPNC does not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retain enzyme activity even when it is linked to INPNC with 6?? His tag. For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>. false false _2400_ 23701 29998 9 true N/A false Tomoki Uchino annotation2490296 1 INPNC range2490296 1 4 957 annotation2490295 1 start codon range2490295 1 1 3 annotation2514825 1 stop codon range2514825 1 976 978 annotation2490297 1 6xHis tag range2490297 1 958 975 BBa_K1933001_sequence 1 atgaccctggacaaagctctggttctgcgtacctgcgctaacaacatggctgaccactgcggtctgatctggccggcttctggtaccgttgaatctcgttactggcagtctacccgtcgtcacgaaaacggtctggttggtctgctgtggggtgctggtacctctgctttcctgtctgttcacgctgacgctcgttggatcgtttgcgaagttgctgttgctgacatcatctctctggaagaaccgggtatggttaaattcccgcgtgctgaagttgttcacgttggtgaccgtatctctgcttctcacttcatctctgctcgtcaggctgacccggcttctacctctacctctacctctacctctaccctgaccccgatgccgaccgctatcccgaccccgatgccggctgttgcttctgttaccctgccggttgctgaacaggctcgtcacgaagttttcgacgttgcttctgtttctgctgctgctgctccggttaacaccctgccggttaccaccccgcagaacctgcaaaccgctacctacggttctaccctgtctggtgacaaccactctcgtctgatcgctggttacggttctaacgaaaccgctggtaaccactctgacctgatcggtggtcacgactgcaccctgatggctggtgaccagtctcgtctgaccgctggtaaaaactctgttctgaccgctggtgctcgttctaaactgatcggttctgaaggttctaccctgtctgctggtgaagactctaccctgatcttccgtctgtgggacggtaaacgttaccgtcagctggttgctcgtaccggtgaaaacggtgttgaagctgacatcccgtactacgttaacgaagacgacgacatcgttgacaaaccggacgaagacgacgactggatcgaagttaaaggtggcggtagtggcggtgcaggaggcggatcccatcatcatcatcatcattag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z