BBa_K1933002
1
scFv
anti-Norovirus GII.4 scFv
2016-10-09T11:00:00Z
2016-10-18T10:28:59Z
We have been given given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens. [1] This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project.
For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>.
false
false
_2400_
23701
29998
9
true
silent mutation was made to remove PstI: G255C
false
Tomoki Uchino
annotation2490183
1
VH
range2490183
1
4
392
annotation2490182
1
start codon
range2490182
1
1
3
annotation2490184
1
linker
range2490184
1
393
438
annotation2490185
1
VL
range2490185
1
439
777
annotation2490197
1
G255C
range2490197
1
255
255
annotation2490186
1
stop codon
range2490186
1
778
780
BBa_K1933002_sequence
1
atggccgaggtgcagctggtggagtctgggggaggcttggtacagcctggggggtccctgagactctcctgtgcaacctctgcattcatctttgacaactatcccatgacttgggtccgccaggctcaggctccagggaaggggctggagtgggtcgcaagtattagtggtagtggcggcaggacatactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagtatgtgttgtttctccagatgaacagcctgagagccgaggacacggccatatattactgtgtgaaatctcctcccgacgtttgggggagtcatcaattcagggattggtactttgactactggggccagggaaccctggtcaccgtctcgagaggcggtggcggatcaggtggcggtggaagtggcggtggtgggtccatggcctcctatgtgctgactcagccaccctcagcgtctgggacccccgggcagagggtcaccatctcttgttctggaagcagctccaacatcggaagtaatactataaactggtaccagcagctcccaggaacggcccccaaactcctcatctataataatcatcagcggccctcaggggtccctgaccgattctctggctcaaagtctggcacctcagcctccctggccatcagtgggctccagtctgcggatgaggctgattattactgtggagcgtggaatgacagcctgaatgtctatgtcttcggaactgggaccaaggtcaccgtcctaggttag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z