LOCUS       BBa_K1933103             444 bp    DNA     linear       22-Nov-2024
DEFINITION  constitutive expression of CBDclos fused to BclA with 6xHis tag
ACCESSION   BBa_K1933103
VERSION     BBa_K1933103.1  
FEATURES             Location/Qualifiers
     misc_feature    36..42
                     /label=nonsense sequence
     promoter        1..35
                     /label=Re-entry of BBa_J23105
     RBS             43..54
                     /label=Re-entry of BBa_B0034
     misc_feature    55..60
                     /label=scar
     misc_feature    1..60
                     /label=constitutive promoter and RBS
     promoter        1..60
                     /label=BBa_K1933004
     misc_feature    61..63
                     /label=start codon
     CDS             64..126
                     /label=BclA
     misc_feature    127..144
                     /label=6xHis tag
     CDS             61..144
                     /label=BBa_K1933000
     CDS             145..444
                     /label=BBa_K1321002
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagcactag agaaagagga gaaatactag
       61 atggcgttcg acccgaacct ggttggtccg accctgccgc cgatcccgcc gttcaccctg
      121 ccgacccatc atcatcatca tcattcatca atgtcagttg aattttacaa ctctaacaaa
      181 tcagcacaaa caaactcaat tacaccaata atcaaaatta ctaacacgtc tgacagtgat
      241 ttaaatttaa atgacgtaaa agttagatat tattacacaa gtgatggtac acaaggacaa
      301 actttctggt gtgaccatgc tggtgcatta ttaggaaata gctatgttga taacactagc
      361 aaagtgacag caaacttcgt taaagaaaca gcaagcccaa catcaaccta tgatacatat
      421 gttgaatttg gatttgcagg cagc
//