LOCUS       BBa_K1933200            1815 bp    DNA     linear       31-Oct-2024
DEFINITION  constitutive expression of anti-Norovirus GII.4 scFv fused to 
            INPNC with 6xHis tag
ACCESSION   BBa_K1933200
VERSION     BBa_K1933200.1  
FEATURES             Location/Qualifiers
     misc_feature    36..42
                     /label=nonsense sequence
     promoter        1..35
                     /label=Re-entry of BBa_J23105
     RBS             43..54
                     /label=Re-entry of BBa_B0034
     misc_feature    55..60
                     /label=scar
     misc_feature    1..60
                     /label=constitutive promoter and RBS
     promoter        1..60
                     /label=BBa_K1933004
     misc_feature    61..63
                     /label= start codon
     CDS             64..1017
                     /label=INPNC
     misc_feature    1018..1035
                     /label=6xHis tag
     CDS             61..1035
                     /label=BBa_K1933011
     misc_feature    1036..1038
                     /label=start codon
     CDS             1039..1427
                     /label=VH
     CDS             1428..1473
                     /label=linker
     CDS             1474..1812
                     /label=VL
     misc_feature    1813..1815
                     /label=stop codon
     misc_feature    1290..1290
                     /label=G255C
     CDS             1036..1815
                     /label=BBa_K1933002
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagcactag agaaagagga gaaatactag
       61 atgaccctgg acaaagctct ggttctgcgt acctgcgcta acaacatggc tgaccactgc
      121 ggtctgatct ggccggcttc tggtaccgtt gaatctcgtt actggcagtc tacccgtcgt
      181 cacgaaaacg gtctggttgg tctgctgtgg ggtgctggta cctctgcttt cctgtctgtt
      241 cacgctgacg ctcgttggat cgtttgcgaa gttgctgttg ctgacatcat ctctctggaa
      301 gaaccgggta tggttaaatt cccgcgtgct gaagttgttc acgttggtga ccgtatctct
      361 gcttctcact tcatctctgc tcgtcaggct gacccggctt ctacctctac ctctacctct
      421 acctctaccc tgaccccgat gccgaccgct atcccgaccc cgatgccggc tgttgcttct
      481 gttaccctgc cggttgctga acaggctcgt cacgaagttt tcgacgttgc ttctgtttct
      541 gctgctgctg ctccggttaa caccctgccg gttaccaccc cgcagaacct gcaaaccgct
      601 acctacggtt ctaccctgtc tggtgacaac cactctcgtc tgatcgctgg ttacggttct
      661 aacgaaaccg ctggtaacca ctctgacctg atcggtggtc acgactgcac cctgatggct
      721 ggtgaccagt ctcgtctgac cgctggtaaa aactctgttc tgaccgctgg tgctcgttct
      781 aaactgatcg gttctgaagg ttctaccctg tctgctggtg aagactctac cctgatcttc
      841 cgtctgtggg acggtaaacg ttaccgtcag ctggttgctc gtaccggtga aaacggtgtt
      901 gaagctgaca tcccgtacta cgttaacgaa gacgacgaca tcgttgacaa accggacgaa
      961 gacgacgact ggatcgaagt taaaggtggc ggtagtggcg gtgcaggagg cggatcccat
     1021 catcatcatc atcatatggc cgaggtgcag ctggtggagt ctgggggagg cttggtacag
     1081 cctggggggt ccctgagact ctcctgtgca acctctgcat tcatctttga caactatccc
     1141 atgacttggg tccgccaggc tcaggctcca gggaaggggc tggagtgggt cgcaagtatt
     1201 agtggtagtg gcggcaggac atactacgca gactccgtga agggccgctt caccatctcc
     1261 agagacaatt ccaagtatgt gttgtttctc cagatgaaca gcctgagagc cgaggacacg
     1321 gccatatatt actgtgtgaa atctcctccc gacgtttggg ggagtcatca attcagggat
     1381 tggtactttg actactgggg ccagggaacc ctggtcaccg tctcgagagg cggtggcgga
     1441 tcaggtggcg gtggaagtgg cggtggtggg tccatggcct cctatgtgct gactcagcca
     1501 ccctcagcgt ctgggacccc cgggcagagg gtcaccatct cttgttctgg aagcagctcc
     1561 aacatcggaa gtaatactat aaactggtac cagcagctcc caggaacggc ccccaaactc
     1621 ctcatctata ataatcatca gcggccctca ggggtccctg accgattctc tggctcaaag
     1681 tctggcacct cagcctccct ggccatcagt gggctccagt ctgcggatga ggctgattat
     1741 tactgtggag cgtggaatga cagcctgaat gtctatgtct tcggaactgg gaccaaggtc
     1801 accgtcctag gttag
//