BBa_K1933200
1
BBa_K1933200
constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag
2016-10-09T11:00:00Z
2016-10-17T04:57:35Z
Promoter and RBS
The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. scFv is made from fusing variable region (VH and VL chain) of a human anti-norovirus antibody with a linker peptide. We have been given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a single chained variable fragment(scFv) of a norovirus GII.4 strain fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Western blotting. For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>.
false
false
_2400_
23701
29998
9
true
Silent mutation was done to remove PstI site.
false
Tomoki Uchino
component2515638
1
BBa_K1933002
component2515627
1
BBa_K1933004
component2515631
1
BBa_K1933011
annotation2515638
1
BBa_K1933002
range2515638
1
1036
1815
annotation2515627
1
BBa_K1933004
range2515627
1
1
60
annotation2515631
1
BBa_K1933011
range2515631
1
61
1035
BBa_K1933011
1
BBa_K1933011
INPNC fused to 6xHis tag without stop codon
2016-10-16T11:00:00Z
2016-10-17T01:49:52Z
INPNC derived from BBa_K811005
INPNC codes for N- and C- terminal domain of Ice-Nucleation Protein (INP) from Pseudomonas syringae , connected by a 6xHis tag to be easily identified by Western blotting[1]. This is used as an anchoring motif for the cell surface display of passengers. This domain does not have signal peptide for anchoring on the cell surface[2]. Surface displayed passengers retain enzyme activity even when it is linked to INPNC with 6xHis tag.
false
false
_2400_
23701
23701
9
false
We omitted stop codon from BBa_K1933001 to use it for the registration of composite parts.
false
Sora Matsumoto, Tomoki Uchino
annotation2514865
1
start codon
range2514865
1
1
3
annotation2514868
1
6xHis tag
range2514868
1
958
975
annotation2514867
1
INPNC
range2514867
1
4
957
BBa_K1933002
1
scFv
anti-Norovirus GII.4 scFv
2016-10-09T11:00:00Z
2016-10-18T10:28:59Z
We have been given given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens. [1] This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project.
For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>.
false
false
_2400_
23701
29998
9
true
silent mutation was made to remove PstI: G255C
false
Tomoki Uchino
annotation2490182
1
start codon
range2490182
1
1
3
annotation2490183
1
VH
range2490183
1
4
392
annotation2490185
1
VL
range2490185
1
439
777
annotation2490184
1
linker
range2490184
1
393
438
annotation2490186
1
stop codon
range2490186
1
778
780
annotation2490197
1
G255C
range2490197
1
255
255
BBa_K1933004
1
BBa_K1933004
constitutive promoter and RBS
2016-10-09T11:00:00Z
2016-10-10T09:38:21Z
BBa_J23105 was designed by John Anderson and BBa_B0034 was designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. This sequence was chemically synthesized.
This part codes costitutive promoter(BBa_J23105) and RBS(BBa_B0034). Most parts were expressioned by this part in our project.
false
false
_2400_
29998
29998
9
false
7bp after promoter and 6bp after RBS
false
Tomoki Uchino
annotation2490322
1
nonsense sequence
range2490322
1
36
42
annotation2490323
1
Re-entry of BBa_J23105
range2490323
1
1
35
annotation2490408
1
constitutive promoter and RBS
range2490408
1
1
60
annotation2490405
1
scar
range2490405
1
55
60
annotation2490404
1
Re-entry of BBa_B0034
range2490404
1
43
54
BBa_K1933200_sequence
1
tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactagatgaccctggacaaagctctggttctgcgtacctgcgctaacaacatggctgaccactgcggtctgatctggccggcttctggtaccgttgaatctcgttactggcagtctacccgtcgtcacgaaaacggtctggttggtctgctgtggggtgctggtacctctgctttcctgtctgttcacgctgacgctcgttggatcgtttgcgaagttgctgttgctgacatcatctctctggaagaaccgggtatggttaaattcccgcgtgctgaagttgttcacgttggtgaccgtatctctgcttctcacttcatctctgctcgtcaggctgacccggcttctacctctacctctacctctacctctaccctgaccccgatgccgaccgctatcccgaccccgatgccggctgttgcttctgttaccctgccggttgctgaacaggctcgtcacgaagttttcgacgttgcttctgtttctgctgctgctgctccggttaacaccctgccggttaccaccccgcagaacctgcaaaccgctacctacggttctaccctgtctggtgacaaccactctcgtctgatcgctggttacggttctaacgaaaccgctggtaaccactctgacctgatcggtggtcacgactgcaccctgatggctggtgaccagtctcgtctgaccgctggtaaaaactctgttctgaccgctggtgctcgttctaaactgatcggttctgaaggttctaccctgtctgctggtgaagactctaccctgatcttccgtctgtgggacggtaaacgttaccgtcagctggttgctcgtaccggtgaaaacggtgttgaagctgacatcccgtactacgttaacgaagacgacgacatcgttgacaaaccggacgaagacgacgactggatcgaagttaaaggtggcggtagtggcggtgcaggaggcggatcccatcatcatcatcatcatatggccgaggtgcagctggtggagtctgggggaggcttggtacagcctggggggtccctgagactctcctgtgcaacctctgcattcatctttgacaactatcccatgacttgggtccgccaggctcaggctccagggaaggggctggagtgggtcgcaagtattagtggtagtggcggcaggacatactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagtatgtgttgtttctccagatgaacagcctgagagccgaggacacggccatatattactgtgtgaaatctcctcccgacgtttgggggagtcatcaattcagggattggtactttgactactggggccagggaaccctggtcaccgtctcgagaggcggtggcggatcaggtggcggtggaagtggcggtggtgggtccatggcctcctatgtgctgactcagccaccctcagcgtctgggacccccgggcagagggtcaccatctcttgttctggaagcagctccaacatcggaagtaatactataaactggtaccagcagctcccaggaacggcccccaaactcctcatctataataatcatcagcggccctcaggggtccctgaccgattctctggctcaaagtctggcacctcagcctccctggccatcagtgggctccagtctgcggatgaggctgattattactgtggagcgtggaatgacagcctgaatgtctatgtcttcggaactgggaccaaggtcaccgtcctaggttag
BBa_K1933002_sequence
1
atggccgaggtgcagctggtggagtctgggggaggcttggtacagcctggggggtccctgagactctcctgtgcaacctctgcattcatctttgacaactatcccatgacttgggtccgccaggctcaggctccagggaaggggctggagtgggtcgcaagtattagtggtagtggcggcaggacatactacgcagactccgtgaagggccgcttcaccatctccagagacaattccaagtatgtgttgtttctccagatgaacagcctgagagccgaggacacggccatatattactgtgtgaaatctcctcccgacgtttgggggagtcatcaattcagggattggtactttgactactggggccagggaaccctggtcaccgtctcgagaggcggtggcggatcaggtggcggtggaagtggcggtggtgggtccatggcctcctatgtgctgactcagccaccctcagcgtctgggacccccgggcagagggtcaccatctcttgttctggaagcagctccaacatcggaagtaatactataaactggtaccagcagctcccaggaacggcccccaaactcctcatctataataatcatcagcggccctcaggggtccctgaccgattctctggctcaaagtctggcacctcagcctccctggccatcagtgggctccagtctgcggatgaggctgattattactgtggagcgtggaatgacagcctgaatgtctatgtcttcggaactgggaccaaggtcaccgtcctaggttag
BBa_K1933004_sequence
1
tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactag
BBa_K1933011_sequence
1
atgaccctggacaaagctctggttctgcgtacctgcgctaacaacatggctgaccactgcggtctgatctggccggcttctggtaccgttgaatctcgttactggcagtctacccgtcgtcacgaaaacggtctggttggtctgctgtggggtgctggtacctctgctttcctgtctgttcacgctgacgctcgttggatcgtttgcgaagttgctgttgctgacatcatctctctggaagaaccgggtatggttaaattcccgcgtgctgaagttgttcacgttggtgaccgtatctctgcttctcacttcatctctgctcgtcaggctgacccggcttctacctctacctctacctctacctctaccctgaccccgatgccgaccgctatcccgaccccgatgccggctgttgcttctgttaccctgccggttgctgaacaggctcgtcacgaagttttcgacgttgcttctgtttctgctgctgctgctccggttaacaccctgccggttaccaccccgcagaacctgcaaaccgctacctacggttctaccctgtctggtgacaaccactctcgtctgatcgctggttacggttctaacgaaaccgctggtaaccactctgacctgatcggtggtcacgactgcaccctgatggctggtgaccagtctcgtctgaccgctggtaaaaactctgttctgaccgctggtgctcgttctaaactgatcggttctgaaggttctaccctgtctgctggtgaagactctaccctgatcttccgtctgtgggacggtaaacgttaccgtcagctggttgctcgtaccggtgaaaacggtgttgaagctgacatcccgtactacgttaacgaagacgacgacatcgttgacaaaccggacgaagacgacgactggatcgaagttaaaggtggcggtagtggcggtgcaggaggcggatcccatcatcatcatcatcat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z