LOCUS       BBa_K1933201             924 bp    DNA     linear       29-Nov-2024
DEFINITION  constitutive expression of anti-Norovirus GII.4 scFv fused to BclA 
            with 6xHis tag
ACCESSION   BBa_K1933201
VERSION     BBa_K1933201.1  
FEATURES             Location/Qualifiers
     misc_feature    36..42
                     /label=nonsense sequence
     promoter        1..35
                     /label=Re-entry of BBa_J23105
     RBS             43..54
                     /label=Re-entry of BBa_B0034
     misc_feature    55..60
                     /label=scar
     misc_feature    1..60
                     /label=constitutive promoter and RBS
     promoter        1..60
                     /label=BBa_K1933004
     misc_feature    61..63
                     /label=start codon
     CDS             64..126
                     /label=BclA
     misc_feature    127..144
                     /label=6xHis tag
     CDS             61..144
                     /label=BBa_K1933000
     misc_feature    145..147
                     /label=start codon
     CDS             148..536
                     /label=VH
     CDS             537..582
                     /label=linker
     CDS             583..921
                     /label=VL
     misc_feature    922..924
                     /label=stop codon
     misc_feature    399..399
                     /label=G255C
     CDS             145..924
                     /label=BBa_K1933002
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagcactag agaaagagga gaaatactag
       61 atggcgttcg acccgaacct ggttggtccg accctgccgc cgatcccgcc gttcaccctg
      121 ccgacccatc atcatcatca tcatatggcc gaggtgcagc tggtggagtc tgggggaggc
      181 ttggtacagc ctggggggtc cctgagactc tcctgtgcaa cctctgcatt catctttgac
      241 aactatccca tgacttgggt ccgccaggct caggctccag ggaaggggct ggagtgggtc
      301 gcaagtatta gtggtagtgg cggcaggaca tactacgcag actccgtgaa gggccgcttc
      361 accatctcca gagacaattc caagtatgtg ttgtttctcc agatgaacag cctgagagcc
      421 gaggacacgg ccatatatta ctgtgtgaaa tctcctcccg acgtttgggg gagtcatcaa
      481 ttcagggatt ggtactttga ctactggggc cagggaaccc tggtcaccgt ctcgagaggc
      541 ggtggcggat caggtggcgg tggaagtggc ggtggtgggt ccatggcctc ctatgtgctg
      601 actcagccac cctcagcgtc tgggaccccc gggcagaggg tcaccatctc ttgttctgga
      661 agcagctcca acatcggaag taatactata aactggtacc agcagctccc aggaacggcc
      721 cccaaactcc tcatctataa taatcatcag cggccctcag gggtccctga ccgattctct
      781 ggctcaaagt ctggcacctc agcctccctg gccatcagtg ggctccagtc tgcggatgag
      841 gctgattatt actgtggagc gtggaatgac agcctgaatg tctatgtctt cggaactggg
      901 accaaggtca ccgtcctagg ttag
//