pLacIQ1-RB
type: ComponentDefinition
persistent identity: BBa K193404
display id: BBa_K193404
version: 1
was generated by: iGEM to SBOL conversion
description: coding a set of enzymes (ho1 and pcyA) producing PCB from heam
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: We used biobrick standard assembly.
owned by: james
owned by: myers
mutable description: this generator codes a series of enzymes needed to oxidize heam group into PCB(phycocyanobilin). Then PCB is associated with cph8 playing a important role in light sensor.
discontinued: false
dominant: true
experience: experience/None
group u list: _288_
m user id: 0
owner id: 4368
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used K193402 and K193403.
star: false
creator: HIroshi Shiba
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K193403
component: BBa_K193401
sequence annotation: BBa_K193401
sequence annotation: BBa_K193403
sequence: https://synbiohub.org/public/igem/BBa_K193404_sequence/1

BBa_K193403
type: Component
persistent identity: component2267666
display id: component2267666
version: 1
top level: pLacIQ1-RB
definition: RBS-pcyA-t
access: public

BBa_K193401
type: Component
persistent identity: component2267651
display id: component2267651
version: 1
top level: pLacIQ1-RB
definition: pLacIQ1-RB
access: public

BBa_K193401
type: SequenceAnnotation
persistent identity: annotation2267651
display id: annotation2267651
version: 1
top level: pLacIQ1-RB
location: https://synbiohub.org/public/igem/BBa_K193404/annotation2267651/range2267651/1
component: BBa_K193401

https://synbiohub.org/public/igem/BBa_K193404/annotation2267651/range2267651/1
type: Range
persistent identity: range2267651
display id: range2267651
version: 1
top level: pLacIQ1-RB
direction: direction/reverse
start: 1
end: 836
orientation: inline

BBa_K193403
type: SequenceAnnotation
persistent identity: annotation2267666
display id: annotation2267666
version: 1
top level: pLacIQ1-RB
location: https://synbiohub.org/public/igem/BBa_K193404/annotation2267666/range2267666/1
component: BBa_K193403

https://synbiohub.org/public/igem/BBa_K193404/annotation2267666/range2267666/1
type: Range
persistent identity: range2267666
display id: range2267666
version: 1
top level: pLacIQ1-RB
direction: direction/reverse
start: 845
end: 1743
orientation: inline

pLacIQ1-RB
type: ComponentDefinition
persistent identity: BBa K193401
display id: BBa_K193401
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 under the control of Plac
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: We used biobrick standard assembly.
owned by: james
owned by: myers
mutable description: Plac IQ, constittutive promoter, is located upstream of RBS-HO1 in this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _288_
m user id: 0
owner id: 4368
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We used K091112, K193400.
star: false
creator: HIroshi Shiba
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K193400
component: BBa_K091112
sequence annotation: BBa_K091112
sequence annotation: BBa_K193400
sequence: https://synbiohub.org/public/igem/BBa_K193401_sequence/1

BBa_K193400
type: Component
persistent identity: component2246693
display id: component2246693
version: 1
top level: pLacIQ1-RB
definition: RBS-ho1
access: public

BBa_K091112
type: Component
persistent identity: component2246687
display id: component2246687
version: 1
top level: pLacIQ1-RB
definition: pLacIQ1
access: public

BBa_K091112
type: SequenceAnnotation
persistent identity: annotation2246687
display id: annotation2246687
version: 1
top level: pLacIQ1-RB
location: https://synbiohub.org/public/igem/BBa_K193401/annotation2246687/range2246687/1
component: BBa_K091112

https://synbiohub.org/public/igem/BBa_K193401/annotation2246687/range2246687/1
type: Range
persistent identity: range2246687
display id: range2246687
version: 1
top level: pLacIQ1-RB
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_K193400
type: SequenceAnnotation
persistent identity: annotation2246693
display id: annotation2246693
version: 1
top level: pLacIQ1-RB
location: https://synbiohub.org/public/igem/BBa_K193401/annotation2246693/range2246693/1
component: BBa_K193400

https://synbiohub.org/public/igem/BBa_K193401/annotation2246693/range2246693/1
type: Range
persistent identity: range2246693
display id: range2246693
version: 1
top level: pLacIQ1-RB
direction: direction/reverse
start: 65
end: 836
orientation: inline

RBS-pcyA-t
type: ComponentDefinition
persistent identity: BBa K193403
display id: BBa_K193403
version: 1
was generated by: iGEM to SBOL conversion
description: teminator is located downstream of the BBa_193402
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: We used biobrick standard assembly.
owned by: james
owned by: myers
mutable description: strong terminator is located downstream of RBS-pcyA
discontinued: false
dominant: false
experience: experience/None
group u list: _288_
m user id: 0
owner id: 4368
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We used K193402 and I15009.
star: false
creator: HIroshi Shiba
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0014
component: BBa_K193402
sequence annotation: BBa_K193402
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K193403_sequence/1

BBa_B0014
type: Component
persistent identity: component2249808
display id: component2249808
version: 1
top level: RBS-pcyA-t
definition: BBa_B0014
access: public

BBa_K193402
type: Component
persistent identity: component2249801
display id: component2249801
version: 1
top level: RBS-pcyA-t
definition: RBS-pcyA
access: public

BBa_K193402
type: SequenceAnnotation
persistent identity: annotation2249801
display id: annotation2249801
version: 1
top level: RBS-pcyA-t
location: https://synbiohub.org/public/igem/BBa_K193403/annotation2249801/range2249801/1
component: BBa_K193402

https://synbiohub.org/public/igem/BBa_K193403/annotation2249801/range2249801/1
type: Range
persistent identity: range2249801
display id: range2249801
version: 1
top level: RBS-pcyA-t
direction: direction/reverse
start: 1
end: 796
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2249808
display id: annotation2249808
version: 1
top level: RBS-pcyA-t
location: https://synbiohub.org/public/igem/BBa_K193403/annotation2249808/range2249808/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K193403/annotation2249808/range2249808/1
type: Range
persistent identity: range2249808
display id: range2249808
version: 1
top level: RBS-pcyA-t
direction: direction/reverse
start: 805
end: 899
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

pLacIQ1
type: ComponentDefinition
persistent identity: BBa K091112
display id: BBa_K091112
version: 1
was generated by: iGEM to SBOL conversion
description: pLacIQ1 promoter
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This is a modified version of the LacIQ promoter.
owned by: james
owned by: myers
mutable description: this is a modified version of the LacIQ promoter that should bind LacIQ tighter than the original.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no special consideration
star: true
creator: Pallavi Penumetcha
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091112_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1964077
display id: annotation1964077
version: 1
top level: pLacIQ1
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1
type: Range
persistent identity: range1964077
display id: range1964077
version: 1
top level: pLacIQ1
direction: direction/forward
start: 12
end: 17
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1964078
display id: annotation1964078
version: 1
top level: pLacIQ1
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1
type: Range
persistent identity: range1964078
display id: range1964078
version: 1
top level: pLacIQ1
direction: direction/forward
start: 37
end: 41
orientation: inline

RBS-ho1
type: ComponentDefinition
persistent identity: BBa K193400
display id: BBa_K193400
version: 1
was generated by: iGEM to SBOL conversion
description: RBS is located upstream of ho1
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: We used B0030 and I15008.
owned by: james
owned by: myers
mutable description: HO1 is a certain enzyme used to oxidize the heam group into biliverdin Ixalpha which is the first process of changing it into PCB(phycocyanobilin). This part both includes RBS and HO1.
discontinued: false
dominant: false
experience: experience/None
group u list: _288_
m user id: 0
owner id: 4368
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We used biobrick standard assembly.
star: false
creator: HIroshi Shiba
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I15008
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I15008
sequence: https://synbiohub.org/public/igem/BBa_K193400_sequence/1

BBa_I15008
type: Component
persistent identity: component2244321
display id: component2244321
version: 1
top level: RBS-ho1
definition: BBa_I15008
access: public

BBa_B0030
type: Component
persistent identity: component2244318
display id: component2244318
version: 1
top level: RBS-ho1
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244318
display id: annotation2244318
version: 1
top level: RBS-ho1
location: https://synbiohub.org/public/igem/BBa_K193400/annotation2244318/range2244318/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K193400/annotation2244318/range2244318/1
type: Range
persistent identity: range2244318
display id: range2244318
version: 1
top level: RBS-ho1
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2244321
display id: annotation2244321
version: 1
top level: RBS-ho1
location: https://synbiohub.org/public/igem/BBa_K193400/annotation2244321/range2244321/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K193400/annotation2244321/range2244321/1
type: Range
persistent identity: range2244321
display id: range2244321
version: 1
top level: RBS-ho1
direction: direction/reverse
start: 22
end: 772
orientation: inline

RBS-pcyA
type: ComponentDefinition
persistent identity: BBa K193402
display id: BBa_K193402
version: 1
was generated by: iGEM to SBOL conversion
description: RBS is located upstream of pcyA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: We used biobrick standard assembly.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pcyA is the enzyme, oxidizing biliverdin Ixalpha into phycocyanobilin which is the second step of changing heam group into phycocyanobilin. RBS is followed by pcyA in this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _288_
m user id: 0
owner id: 4368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We used B0030 and I15009.
star: false
creator: HIroshi Shiba
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I15009
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I15009
sequence: https://synbiohub.org/public/igem/BBa_K193402_sequence/1

BBa_I15009
type: Component
persistent identity: component2244327
display id: component2244327
version: 1
top level: RBS-pcyA
definition: BBa_I15009
access: public

BBa_B0030
type: Component
persistent identity: component2244323
display id: component2244323
version: 1
top level: RBS-pcyA
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244323
display id: annotation2244323
version: 1
top level: RBS-pcyA
location: https://synbiohub.org/public/igem/BBa_K193402/annotation2244323/range2244323/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K193402/annotation2244323/range2244323/1
type: Range
persistent identity: range2244323
display id: range2244323
version: 1
top level: RBS-pcyA
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2244327
display id: annotation2244327
version: 1
top level: RBS-pcyA
location: https://synbiohub.org/public/igem/BBa_K193402/annotation2244327/range2244327/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K193402/annotation2244327/range2244327/1
type: Range
persistent identity: range2244327
display id: range2244327
version: 1
top level: RBS-pcyA
direction: direction/reverse
start: 22
end: 796
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

https://synbiohub.org/public/igem/BBa_K193404_sequence/1
type: Sequence
persistent identity: BBa K193404 sequence
display id: BBa_K193404_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: agcggcatgcatttacgttgacaccacctttcgcggtatggcatgatagcgcccggtactagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K193401_sequence/1
type: Sequence
persistent identity: BBa K193401 sequence
display id: BBa_K193401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcggcatgcatttacgttgacaccacctttcgcggtatggcatgatagcgcccggtactagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K193403_sequence/1
type: Sequence
persistent identity: BBa K193403 sequence
display id: BBa_K193403_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091112_sequence/1
type: Sequence
persistent identity: BBa K091112 sequence
display id: BBa_K091112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcggcatgcatttacgttgacaccacctttcgcggtatggcatgatagcgcccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K193400_sequence/1
type: Sequence
persistent identity: BBa K193400 sequence
display id: BBa_K193400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K193402_sequence/1
type: Sequence
persistent identity: BBa K193402 sequence
display id: BBa_K193402_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z