LOCUS       BBa_K193406             1743 bp    DNA     linear       01-Jun-2024
DEFINITION  the coding is the same as BBa_193404 the back bone is changed onto 
            pSB6A1
ACCESSION   BBa_K193406
VERSION     BBa_K193406.1  
FEATURES             Location/Qualifiers
     promoter        12..17
                     /label=-35
     promoter        37..41
                     /label=-10
     promoter        1..56
                     /label=BBa_K091112
     RBS             65..79
                     /label=RBS-1\Strong
     RBS             72..76
                     /label=RBS
     misc_feature    65..79
                     /label=BBa_B0030
     RBS             65..79
                     /label=BBa_B0030
     misc_feature    812..836
                     /label=Help:Barcodes
     CDS             86..836
                     /label=BBa_I15008
     misc_feature    65..836
                     /label=BBa_K193400
     misc_feature    1..836
                     /label=BBa_K193401
     RBS             845..859
                     /label=RBS-1\Strong
     RBS             852..856
                     /label=RBS
     misc_feature    845..859
                     /label=BBa_B0030
     RBS             845..859
                     /label=BBa_B0030
     CDS             866..1615
                     /label=PcyA
     misc_feature    1616..1640
                     /label=Help:Barcodes
     CDS             866..1640
                     /label=BBa_I15009
     misc_feature    845..1640
                     /label=BBa_K193402
     stem_loop       1656..1675
                     /label=T7 TE
     misc_feature    1682..1682
                     /label=stop
     polyA_site      1676..1689
                     /label=polya
     misc_feature    1649..1689
                     /label=BBa_B0012
     terminator      1649..1689
                     /label=BBa_B0012
     stem_loop       1710..1732
                     /label=stem_loop
     misc_feature    1698..1743
                     /label=BBa_B0011
     terminator      1698..1743
                     /label=BBa_B0011
     terminator      1649..1743
                     /label=BBa_B0014
     misc_feature    845..1743
                     /label=BBa_K193403
     misc_feature    1..1743
                     /label=BBa_K193404
ORIGIN
        1 agcggcatgc atttacgttg acaccacctt tcgcggtatg gcatgatagc gcccggtact
       61 agagattaaa gaggagaaat actagatgag tgtcaactta gcttcccagt tgcgggaagg
      121 gacgaaaaaa tcccactcca tggcggagaa cgtcggcttt gtcaaatgct tcctcaaggg
      181 cgttgtcgag aaaaattcct accgtaagct ggttggcaat ctctactttg tctacagtgc
      241 catggaagag gaaatggcaa aatttaagga ccatcccatc ctcagccaca tttacttccc
      301 cgaactcaac cgcaaacaaa gcctagagca agacctgcaa ttctattacg gctccaactg
      361 gcggcaagaa gtgaaaattt ctgccgctgg ccaagcctat gtggaccgag tccggcaagt
      421 ggccgctacg gcccctgaat tgttggtggc ccattcctac acccgttacc tgggggatct
      481 ttccggcggt caaattctca agaaaattgc ccaaaatgcc atgaatctcc acgatggtgg
      541 cacagctttc tatgaatttg ccgacattga tgacgaaaag gcttttaaaa atacctaccg
      601 tcaagctatg aatgatctgc ccattgacca agccaccgcc gaacggattg tggatgaagc
      661 caatgacgcc tttgccatga acatgaaaat gttcaacgaa cttgaaggca acctgatcaa
      721 ggcgatcggc attatggtgt tcaacagcct cacccgtcgc cgcagtcaag gcagcaccga
      781 agttggcctc gccacctccg aaggctaata acgctgatag tgctagtgta gatcgctact
      841 agagattaaa gaggagaaat actagatggc cgtcactgat ttaagtttga ccaattcttc
      901 cctgatgcct acgttgaacc cgatgattca acagttggcc ctggcgatcg ccgctagttg
      961 gcaaagttta cccctcaagc cctatcaatt gccggaggat ttgggctacg tagaaggccg
     1021 cctggaaggg gaaaagttag tgattgaaaa tcggtgctac caaacgcccc agtttcgcaa
     1081 aatgcatttg gagttggcca aggtgggcaa agggttggat attctccact gtgtaatgtt
     1141 tcctgagcct ttatacggtc tacctttgtt tggctgtgac attgtggccg gccccggtgg
     1201 agtaagtgcg gctattgcgg atctatcccc cacccaaagc gatcgccaat tgcccgcagc
     1261 gtaccaaaaa tcattggcag agctaggcca gccagaattt gagcaacaac gggaattgcc
     1321 cccctgggga gaaatatttt ctgaatattg tttattcatc cgtcccagca atgtcactga
     1381 agaagaaaga tttgtacaaa gggtagtgga ctttttgcaa attcattgtc accaatccat
     1441 cgttgccgaa cccttgtctg aagctcaaac tttggagcac cgtcaggggc aaattcatta
     1501 ctgccaacaa caacagaaaa atgataaaac ccgtcgggta ctggaaaaag cttttgggga
     1561 agcttgggcg gaacggtata tgagccaagt cttatttgat gttatccaat aataactctg
     1621 atagtgctag tgtagatctc tactagagtc acactggctc accttcgggt gggcctttct
     1681 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
     1741 ttt
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