the coding is the same as BBa_193404 the back bone is changed onto pSB6A1
We changed the back bone of BBa_K193404.
The backbone of original generator, Plac IQ-RBS-ho1-RBS-pcyA- double terminator, is replaced by pSB6A1.
coding a set of enzymes (ho1 and pcyA) producing PCB from heam
this generator codes a series of enzymes needed to oxidize heam group into PCB(phycocyanobilin). Then PCB is associated with cph8 playing a important role in light sensor.
Plac IQ, constittutive promoter, is located upstream of RBS-HO1 in this part.
teminator is located downstream of the BBa_193402
strong terminator is located downstream of RBS-pcyA
Double terminator consisting of BBa_B0012 and BBa_B0011
This is a modified version of the LacIQ promoter.
this is a modified version of the LacIQ promoter that should bind LacIQ tighter than the original.
HO1 is a certain enzyme used to oxidize the heam group into biliverdin Ixalpha which is the first process of changing it into PCB(phycocyanobilin). This part both includes RBS and HO1.
pcyA is the enzyme, oxidizing biliverdin Ixalpha into phycocyanobilin which is the second step of changing heam group into phycocyanobilin. RBS is followed by pcyA in this part.
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
Second of two required phycocyanobilin biosynthetic genes.
Conversion of the iGEM parts registry to SBOL2.1