BBa_K1935003 1 BBa_K1935003 White collar-1 (WC-1) - photoreceptor in Neurospora tetrasperma 2016-10-12T11:00:00Z 2016-10-13T06:15:26Z http://www.uniprot.org/uniprot/F8MUC4 White Collar-1 (wc-1) is a gene in Neurospora tetrasperma encoding the protein WC-1. WC-1 has two separate roles in the cell. First, it is the primary photoreceptor for Neurospora and the founding member of the class of principle blue light photoreceptors in all of the fungi. Second, it is necessary for regulating circadian rhythms in FRQ. It is a key component of a circadian molecular pathway that regulates many behavioral activities, including conidiation. WC-1 and WC-2, an interacting partner of WC-1, comprise the White Collar Complex (WCC) that is involved in the Neurospora circadian clock. WCC is a complex of nuclear transcription factor proteins, and contains transcriptional activation domains, PAS domains, and zinc finger DNA-binding domains. WC-1 and WC-2 heterodimerize through their PAS domains to form the White Collar Complex. Photoreception: WC-1 has been shown to be a blue-light photoreceptor, and is a necessary component of the Neurospora light-induced response pathway. In functional Neurospora, the WC-1 LOV domain binds to the flavin adenine dinucleotide (FAD) chromophore, which is responsible for the conversion of light to mechanical energy. FAD displays maximum absorption of light at 450nm, thus explaining WC-1's maximal sensitivity to blue light. Circadian regulation: WCC acts as a positive element in the circadian clock. It serves as an activator of frq gene transcription by binding to two DNA promotor elements in the nucleus: the Clock box (C box) and the Proximal Light-Response Element (PLRE). PLRE is required for maximal light induction, while the C box is required for both maximal light induction and maintaining circadian rhythmicity in constant darkness. In addition, light-activated WCC is shown to induce the transcription of VIVID, a small flavin-binding blue-light photoreceptor that is required for adaptation to light-induced responses in the transcription of light-induced genes, including wc-1 and frq. VIVID is a negative regulator of light responses, although its mechanism is not yet known. Only wc-1 is required for transient light-induction, but both wc-1 and wc-2 are required for the circadian clock to run. Because the core of the clock is based on a rhythmic expression of frq, the acute light-induction pathway provides a direct way to reset the clock. false false _2402_ 25368 25368 9 false Nothing relevant false Emilie Gounin annotation2497349 1 intron range2497349 1 2933 2997 BBa_K1935005 1 BBa_K1935005 White collar-2 (WC-2) - photoreceptor in Neurospora tetrasperma 2016-10-12T11:00:00Z 2016-10-13T06:37:51Z https://www.ncbi.nlm.nih.gov/nucleotide/698997583?report=genbank&log$=nuclalign&blast_rank=3&RID=ZYZ7SCEW014 May function as a transcription factor involved in light regulation. Binds and affects blue light regulation of the al-3 gene. Wc-1 and wc-2 interact via homologous PAS domains, bind to promoters of light regulated genes such as frq, and activate transcription. May bind directly to frq. WC-1 and WC-2 are transcription factors encoded by the genes wc-1 and wc-2. Zinc finger DNA-binding domains allow WC-1 and WC-2 to bind to DNA and act as transcription factors. Both WC-1 and WC-2 have PAS domains that allow them to bind to other proteins. WC-1 and WC-2 typically heterodimerize in vivo to form the White Collar Complex (WCC), which acts as a transcription factor complex. In vitro, WC-1 can also homodimerize with itself and heterodimerize with other PAS proteins. WC-1 and WC-2 bind to the promoter elements of the genes that they transcriptionally activate. Function in the circadian clock. false false _2402_ 25368 25368 9 false A mutagenesis was done to abolish an illegal restriction site (PST1) false Emilie Gounin annotation2497366 1 intron range2497366 1 1556 1641 annotation2497365 1 intron range2497365 1 121 285 BBa_K1537016 1 BBa_K1537016 GSG linker+P2A 2014-09-13T11:00:00Z 2015-05-08T01:10:52Z The part is from porcine teschovirus-1. The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And "GSG linker" is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage. false false _1919_ 0 22415 9 In stock false We have to use this part by synthesis from biological company because the virus is dangerous. false Jie Li annotation2383426 1 GSG linker range2383426 1 1 9 annotation2383427 1 P2A range2383427 1 10 66 BBa_K1935002 1 BBa_K1935002 pUNC-119 : Neuron Specific Promoter of C.elegans 2016-10-12T11:00:00Z 2016-10-13T06:12:20Z References : - Wormbase : http://www.wormbase.org/species/c_elegans/gene/WBGene00006843#01-9g-3 - PROMOTEROME database : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=UNC-119 unc-119 gene promoter of Caenorhabditis elegans basically drives the expression of UNC-119 in all neurons of C.elegans. false false _2402_ 25369 25369 9 false Nothing relevant. false Jean DESCARPENTRIE annotation2497346 1 misc range2497346 1 1 2003 BBa_K1935013 1 BBa_K1935013 White Collar Complex under control of C.elegans's promoter (pUNC119::WC1::P2A::WC2) 2016-10-12T11:00:00Z 2016-10-13T08:33:32Z PROMOTEROME database : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=UNC-119 http://www.uniprot.org/uniprot/F8MUC4 https://www.ncbi.nlm.nih.gov/nucleotide/698997583?report=genbank&log$=nuclalign&blast_rank=3&RID=ZYZ7SCEW014 This construction was made to create the White Collar Complex. This one will be injected in Caenorhabditis elegans. See BBa_K1935002 for details on UNC-119 promoter See BBa_K1935003 for details on WC-1 See BBa_K1537016 for details on 2A peptide See BBa_K1935005 for details on WC-2 false false _2402_ 25368 25368 9 false Nothing relevant false Emilie Gounin component2498045 1 BBa_K1935003 component2498043 1 BBa_K1935002 component2498048 1 BBa_K1537016 component2498051 1 BBa_K1935005 annotation2498048 1 BBa_K1537016 range2498048 1 5573 5638 annotation2498043 1 BBa_K1935002 range2498043 1 1 2003 annotation2498051 1 BBa_K1935005 range2498051 1 5645 7464 annotation2498045 1 BBa_K1935003 range2498045 1 2010 5564 BBa_K1935003_sequence 1 atgaacaacaactactacggttccccgctgtctcctgaggagcttcagcatcaaatgcaccagcaccagcagcaacaacaacaacagcagcagcagcagcaacaacaacaacaacaacaacaacaacaacaacagcagcaacaacagcatcagcatcagcagcaacaaaataccaatcaacatcgcaatgtcggcatgatgaatacgcctccaactacaaatcaaggaaacaacacgattcacgctccagatgtaaccatgtcagtaggaagcgactctcttgacgaaatcatccagcagaacctcgacgaaatgcatcgaaggcggagtgtcccccagccctatgggggccagaataggaggttgtccatgtttgattacgcgaaccccaacgatggtttctcggactatcaactcgataatatgtcaggaacttatggggacatgactggcggcatgggcatgtcagggcactcgggcccgtatgcagggcagaacatcatggccatgtcggaccacagcggtggatactcgcacatgtcgcccaatgttatgggcaacatgatgacttaccccaacctcaacatgtaccactctcccccgatcgagaacccctattcttcggccggactcgacactattcgcaccgacttttccatggacatgaacgtggatagtggctccgtcagcgcagccagcgttcatcccacccctgggcttaataagcaggacgacgagatgatgaccatggagcagggctttggtggaggtgacgatgccaacgcctctcaccaggcccaacaaaacatgggcggtttgactccggctatgactccggccatgacccctgcaatgacgccgggtgtcagcaacttcgcacaaggcatggctacaccagtctcacaggatgcggcaagcacgcccgcgacaaccttccagtctccgtccttatccgcaacaatacagacaatacgaattgggccgccgccaccgccgagcgtcaccaatgcccccaccccagcgccatctacctcgacgccgtcaggtggaggagcgtcccagacaaagagcatttactccaaaagcggtttcgacatgctgagggcgttgtggtatgtggcgtcgaggaaggacccaaagctcaagctcggtgccgtggacatgtcttgtgcgtttgtcgtctgtgatgtaacactcaacgattgccccatcatttatgtgtccgataacttccagaacctcacgggctatagtcgccacgagattgtcgggcggaactgccgcttcttgcaggcccccgacggtaatgtggaggctggcaccaagcgcgaatttgtcgaaaacaacgccgtatatacgctcaagaagaccatcgcggagggccaggagattcagcagagtctgatcaattatcggaagggcggcaagccttttctgaatctgttgaccatgattcccattccctgggataccgaagaaatccgttatttcattggatttcagatcgatctcgtggaatgccctgatgccataatcggccaggaaggaaatgggcccatgcaggtcaactatacccacagcgatatcgggcagtacatctggacgccacctacccagaagcaactggagcctgccgacggccagactttgggcgttgatgatgtttcgacacttttgcagcaatgtaattcgaagggtgttgcatccgactggcacaaacaatcctgggataagatgcttctggagaacgcagacgatgttgtccatgtgttgtcgctcaagggtctcttcttatacctatctccggcctgtaagaaagtgttggagtacgatgccagcgacctcgttgggacctcactgtcctcgatttgccacccgtccgacatcgtgccggtgacgagggagttgaaagaggcccagcaacacactccagtcaacatagtcttcagaatccggagaaagaacagtggttatacctggtttgaaagccacggaacattgttcaacgaacagggtaagggtcgcaagtgtatcattcttgtggggagaaaacgccccgtttttgctctacacagaaaggacctcgaactcaacggcggtattggtgacagcgaaatttggaccaaggtctcgacatcgggcatgtttttgtttgtttcatcgaatgtccgatcgctcctggatttgctcccggagaacctccaaggtaccagcatgcaagatctcatgcgcaaggaatccagagccgagtttgggaggacgatagagaaagctagaaagggcaagatcgcgagctgcaagcatgaggtgcaaaacaagcgtggccaggtgctccaggcctatacaacattctaccccggagatggtggtgagggtcaaaggccaacgttcttgttggcgcaaacaaagctgttgaaggcgtcatcaaggacactagctcccgcgaccgtaacggtcaagaatatgtctccgggcggtgttcctttgtcgccaatgcaagggatccagaccgacagtgatagtaacagtcttatgggcgggaggtcgaagtcgggaagcagcgatagtacgggggccatggtctcagctcgtagcagtgcagcacccggccaggatgcggctttggacgccgataatatatttgacgaactcaagacaacgcgttgcaccagctggcagtacgagctgaggcaaatggagaaggtgaatcgcatgttggccgaagagctggcgcagcttttgtctaacaagaaaaaacggaaacgaagaaagggaggaggtaacatggtgagggactgtgcaaattgccatacgaggaacacgcccgaatggcggcgtgggccaagcgggaatcgagatctatgcaacagttgtgggttgagatgggcaaagcaggtaagctatcaattttcttctttggatttgtttgcccccacgttactaatgtggaaaaatggatagactgggcgcgtatcgccgcgcacctcatcccgaggtgggaatggtgattcgatgagcaagaagagcaattcacctagtcactcgtcgccccttcatcgagaagtcggcaacgattcaccgtctactaccaccgctaccaagaactcaccctctcttcgaggaagtagcaccactgcacccggtacgatcacaacggacagcggaccagcggtcgcatcgagcgctagtggaactggtagtacgactatagctacttcagccaattctgctgcatcaaccgtcaacgccctgggccctcctgcgacaggtcccagtggcggctcaccggctcaacatcttccgcctcatctccagggaacacaccttaacgcccaagccatgcagcgtgtacagcagcacaagcagcatcagcagcatcaacaacagcatcaacagcagcatcaacagcagcatcaacagcttcagcagcttcagttcaaccctccacaaagccagccactcctggaaggcggaagcggtttccgtggaagcggaatggagatgacctcaattagagaagagatgggcgaacatcaacagggcttaagtgtatag BBa_K1537016_sequence 1 ggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtcca BBa_K1935005_sequence 1 atgtctcacggacagcctccccctggatccagcatgtatggctttggagccatggggatgggatccggtatggaaactggcatgggaaccggcatgggaaccggcatgtcagcctcgcaggtgcgcagcagcagcaagcagcagcaagcagcattttcttttccgtcagatttttttttctctcctttttttcaagagagaaaaaaaaaaaaattgtttctctttgctggacgaaaatggtgagcaagctaacgattcgccgattgattggctttcaagctgtagatgacttccgacccacaggacatgatgtcgcttctggatacttcagtgtttcctgggtttgacggaatgtcgatgagtctcgatgttggcgattccatgtcaaaccccttcaccccggtctcggtcccgccacccctcccagcaggtaatgcagggcccagtcatgttggggtgtgcggcggtcacggggcacccgaccagctattttcccccgacgaccttattgcgaccagcatgtccagcgccggaccgatgatcgccacgcctaccaccaccacctccggaccttcgggcggcccttcgtcgggcggcgggagcaccctgaccgaatttaccaagcgccgcaattggcccgccaaggttgtcgaagagctccaggactgggaacacattctggatgccaacggacggatcaagcatgtttcgcccagcgtagagcccttgaccggctataagccgcccgaaatcattgacttgttcctcagagacctgatccacccggatgacgtcggggtcttcaccgcggagttgaacgaagccattgcgacgggatcccaattgcgactgttctaccggttccggaaaaaggatggaaactggaccattttcgagactgttggtcatgcgcacatcgccgccgccaagtttgcacccaatccacagaaccagtcgcctttctgccaggccgttttcatgatggctcgaccctacccgaccaagaacgcgggtctgttggattcctttctggaacacaagattgagaacgagagactgaagcgcaggattgccgagctgcgcagagaggagcaggaggagcaggaagagtcgcagaggacatggcgaatgagccaggagggacgatctgatgttacgccgtccgacgataccgcgacccagatgggaatgacgcccttctacattcccatgaatgcccaagctgacgttatgatgccaccacccagccagccggcgtcgtcgctcaacattgcgctgacgcgggagaacctggaaggcattgccggaagccggccggattctatacgcgaaaagatgttgcgttacgagggcaaccatgcagacacgattgaaatgcttacggggctcaagtaccaggagggtgaaagaagtcatggtatcacaacgggtaatgccagcccgacgctcattaagggtgatgcgggaatcgcgattcccctagaccgagatccccggacgggagagaagaagaagaagatcaaggttgcagaggaatacgtgtgcaccgactgcggtaattaccccttctccatattatataacccgctcccaaaatagcttcttgatgactaccagttgctgacaaggatgctgaaacaggtacgctcgattcccccgaatggcgaaaaggccctagtggacccaagacactatgtaatgcctgcggtcttcgctgggcaaagaaagagaaaaagaagaacgccaacaacaacaataacggcggaggtataggcggtcacaacgacatccatacacccatgggcgatcatatgggatag BBa_K1935002_sequence 1 gtgtcaagtctctcaaaattcagttttttaaaccactcctatagtcctatagtcctatagttacccatgaaatccttatatattactgtaaaatgtttcaaaaaccattggcaaattgccagaactgaaaatttccggcaaattggggaaccggcaaattgccaatttgctgaatttgccggaaaaacggtaattgccgaaagtttttgacacgaaaatggcaaattgtggttttaaaatttttttttttggaaatttcagaatttcaattttaatcggcaaaactgtaggcatcctaagaatgttcctacatctattttgaaaagtaagcgaactctatgaaaatgtctaaagaaaatggggaaacaatttcaaaaaggcacagtttcaagtgtttccgaattatactaaatccctctaaaaacttccggcaaattgatatccgtaaaagagcaaatccgcatttttgccgaaaattaaaatttccgacaaatcggcaaaccggcaatttggcgaaatttgccggaaggcaattgccgcccacccctgttccagaggttcaaactggtagcaaagctcaaaatttctcaaattctccaatttttttttgaattttggcagtgtaccaaaatgacattcagtcatattggtttattatagatttatttagataaaatcctaaatgattctacctttaaagatgcccactttaaaagtaatgactcaaacttcaaattgctctaagattctattgaattaccatcttttcctctcattttctctcactgtctatttcatcacaaattcatccctctctccctctctttctctctccctctctctctttctctttgctcatcatctgtcattttgtccgttcctctctctgcgccctcagcgttccccacactctctcgcttctcttttcctagacgtcttcttttttcatcttcttcagccctttttcgccattttccatctctgtcaatcattacggacgacccccattaatttttggatgtgctctgcgagcaagagcacacggaactcatttattttttgttggaatttctttttcattttagttagttaaaaggttattcagtgcaaaaattgattttttgctcttttggtacattagaggggtttgaaagttttccataaaaagggtttccggagtaaaaaatctaaaaatttttacaaacatttccaaaaattttgggaacatatcttttcaaaaactcaaaaaaaaacgcaaacatcataaatttaatcaattaattggatgcgttcaaatattttaaaaatatttttgaattgcatatttctttactcataaaaaaattattaatgtttaagtaaaaaaaatatttcaggattttaaacaaaaactgtaaatcccataaaacagtgttattttttagttatttttagtaattatttgtgaattaaaaacacattttttttcaaaatattccttgaataaataaaattgaaagtgaactcaaaatatttcaccaattttacaaaatttgaaaatttgaaaaaatattttctaccaaattgtgctcaaaaaatattattaagttcccaggaaatttatgatgaaaacttggaatatttttggtaaaattttctcaaaaaattgaaagttgtcaaaaacacactaacactatacctcgcggcatagaaaaaactggtggccgaattttttaaaccaatttaaattaaatatatttttgcactttgtacaaaaaaatttgaagttcccaggaaattcatacggaaatattttccagaacttttataatttttattttaattctcaaaatttacaataaaaacttggaaacctgaaatttgcttaaagaataaccctatcccccacggcctagaaaatactggtggccgaattttttccgcagccacaccacctctaatctccagagcactctccaaaattcccataatccccaaaattttcagctacaacagcatatatg BBa_K1935013_sequence 1 gtgtcaagtctctcaaaattcagttttttaaaccactcctatagtcctatagtcctatagttacccatgaaatccttatatattactgtaaaatgtttcaaaaaccattggcaaattgccagaactgaaaatttccggcaaattggggaaccggcaaattgccaatttgctgaatttgccggaaaaacggtaattgccgaaagtttttgacacgaaaatggcaaattgtggttttaaaatttttttttttggaaatttcagaatttcaattttaatcggcaaaactgtaggcatcctaagaatgttcctacatctattttgaaaagtaagcgaactctatgaaaatgtctaaagaaaatggggaaacaatttcaaaaaggcacagtttcaagtgtttccgaattatactaaatccctctaaaaacttccggcaaattgatatccgtaaaagagcaaatccgcatttttgccgaaaattaaaatttccgacaaatcggcaaaccggcaatttggcgaaatttgccggaaggcaattgccgcccacccctgttccagaggttcaaactggtagcaaagctcaaaatttctcaaattctccaatttttttttgaattttggcagtgtaccaaaatgacattcagtcatattggtttattatagatttatttagataaaatcctaaatgattctacctttaaagatgcccactttaaaagtaatgactcaaacttcaaattgctctaagattctattgaattaccatcttttcctctcattttctctcactgtctatttcatcacaaattcatccctctctccctctctttctctctccctctctctctttctctttgctcatcatctgtcattttgtccgttcctctctctgcgccctcagcgttccccacactctctcgcttctcttttcctagacgtcttcttttttcatcttcttcagccctttttcgccattttccatctctgtcaatcattacggacgacccccattaatttttggatgtgctctgcgagcaagagcacacggaactcatttattttttgttggaatttctttttcattttagttagttaaaaggttattcagtgcaaaaattgattttttgctcttttggtacattagaggggtttgaaagttttccataaaaagggtttccggagtaaaaaatctaaaaatttttacaaacatttccaaaaattttgggaacatatcttttcaaaaactcaaaaaaaaacgcaaacatcataaatttaatcaattaattggatgcgttcaaatattttaaaaatatttttgaattgcatatttctttactcataaaaaaattattaatgtttaagtaaaaaaaatatttcaggattttaaacaaaaactgtaaatcccataaaacagtgttattttttagttatttttagtaattatttgtgaattaaaaacacattttttttcaaaatattccttgaataaataaaattgaaagtgaactcaaaatatttcaccaattttacaaaatttgaaaatttgaaaaaatattttctaccaaattgtgctcaaaaaatattattaagttcccaggaaatttatgatgaaaacttggaatatttttggtaaaattttctcaaaaaattgaaagttgtcaaaaacacactaacactatacctcgcggcatagaaaaaactggtggccgaattttttaaaccaatttaaattaaatatatttttgcactttgtacaaaaaaatttgaagttcccaggaaattcatacggaaatattttccagaacttttataatttttattttaattctcaaaatttacaataaaaacttggaaacctgaaatttgcttaaagaataaccctatcccccacggcctagaaaatactggtggccgaattttttccgcagccacaccacctctaatctccagagcactctccaaaattcccataatccccaaaattttcagctacaacagcatatatgtactagatgaacaacaactactacggttccccgctgtctcctgaggagcttcagcatcaaatgcaccagcaccagcagcaacaacaacaacagcagcagcagcagcaacaacaacaacaacaacaacaacaacaacaacagcagcaacaacagcatcagcatcagcagcaacaaaataccaatcaacatcgcaatgtcggcatgatgaatacgcctccaactacaaatcaaggaaacaacacgattcacgctccagatgtaaccatgtcagtaggaagcgactctcttgacgaaatcatccagcagaacctcgacgaaatgcatcgaaggcggagtgtcccccagccctatgggggccagaataggaggttgtccatgtttgattacgcgaaccccaacgatggtttctcggactatcaactcgataatatgtcaggaacttatggggacatgactggcggcatgggcatgtcagggcactcgggcccgtatgcagggcagaacatcatggccatgtcggaccacagcggtggatactcgcacatgtcgcccaatgttatgggcaacatgatgacttaccccaacctcaacatgtaccactctcccccgatcgagaacccctattcttcggccggactcgacactattcgcaccgacttttccatggacatgaacgtggatagtggctccgtcagcgcagccagcgttcatcccacccctgggcttaataagcaggacgacgagatgatgaccatggagcagggctttggtggaggtgacgatgccaacgcctctcaccaggcccaacaaaacatgggcggtttgactccggctatgactccggccatgacccctgcaatgacgccgggtgtcagcaacttcgcacaaggcatggctacaccagtctcacaggatgcggcaagcacgcccgcgacaaccttccagtctccgtccttatccgcaacaatacagacaatacgaattgggccgccgccaccgccgagcgtcaccaatgcccccaccccagcgccatctacctcgacgccgtcaggtggaggagcgtcccagacaaagagcatttactccaaaagcggtttcgacatgctgagggcgttgtggtatgtggcgtcgaggaaggacccaaagctcaagctcggtgccgtggacatgtcttgtgcgtttgtcgtctgtgatgtaacactcaacgattgccccatcatttatgtgtccgataacttccagaacctcacgggctatagtcgccacgagattgtcgggcggaactgccgcttcttgcaggcccccgacggtaatgtggaggctggcaccaagcgcgaatttgtcgaaaacaacgccgtatatacgctcaagaagaccatcgcggagggccaggagattcagcagagtctgatcaattatcggaagggcggcaagccttttctgaatctgttgaccatgattcccattccctgggataccgaagaaatccgttatttcattggatttcagatcgatctcgtggaatgccctgatgccataatcggccaggaaggaaatgggcccatgcaggtcaactatacccacagcgatatcgggcagtacatctggacgccacctacccagaagcaactggagcctgccgacggccagactttgggcgttgatgatgtttcgacacttttgcagcaatgtaattcgaagggtgttgcatccgactggcacaaacaatcctgggataagatgcttctggagaacgcagacgatgttgtccatgtgttgtcgctcaagggtctcttcttatacctatctccggcctgtaagaaagtgttggagtacgatgccagcgacctcgttgggacctcactgtcctcgatttgccacccgtccgacatcgtgccggtgacgagggagttgaaagaggcccagcaacacactccagtcaacatagtcttcagaatccggagaaagaacagtggttatacctggtttgaaagccacggaacattgttcaacgaacagggtaagggtcgcaagtgtatcattcttgtggggagaaaacgccccgtttttgctctacacagaaaggacctcgaactcaacggcggtattggtgacagcgaaatttggaccaaggtctcgacatcgggcatgtttttgtttgtttcatcgaatgtccgatcgctcctggatttgctcccggagaacctccaaggtaccagcatgcaagatctcatgcgcaaggaatccagagccgagtttgggaggacgatagagaaagctagaaagggcaagatcgcgagctgcaagcatgaggtgcaaaacaagcgtggccaggtgctccaggcctatacaacattctaccccggagatggtggtgagggtcaaaggccaacgttcttgttggcgcaaacaaagctgttgaaggcgtcatcaaggacactagctcccgcgaccgtaacggtcaagaatatgtctccgggcggtgttcctttgtcgccaatgcaagggatccagaccgacagtgatagtaacagtcttatgggcgggaggtcgaagtcgggaagcagcgatagtacgggggccatggtctcagctcgtagcagtgcagcacccggccaggatgcggctttggacgccgataatatatttgacgaactcaagacaacgcgttgcaccagctggcagtacgagctgaggcaaatggagaaggtgaatcgcatgttggccgaagagctggcgcagcttttgtctaacaagaaaaaacggaaacgaagaaagggaggaggtaacatggtgagggactgtgcaaattgccatacgaggaacacgcccgaatggcggcgtgggccaagcgggaatcgagatctatgcaacagttgtgggttgagatgggcaaagcaggtaagctatcaattttcttctttggatttgtttgcccccacgttactaatgtggaaaaatggatagactgggcgcgtatcgccgcgcacctcatcccgaggtgggaatggtgattcgatgagcaagaagagcaattcacctagtcactcgtcgccccttcatcgagaagtcggcaacgattcaccgtctactaccaccgctaccaagaactcaccctctcttcgaggaagtagcaccactgcacccggtacgatcacaacggacagcggaccagcggtcgcatcgagcgctagtggaactggtagtacgactatagctacttcagccaattctgctgcatcaaccgtcaacgccctgggccctcctgcgacaggtcccagtggcggctcaccggctcaacatcttccgcctcatctccagggaacacaccttaacgcccaagccatgcagcgtgtacagcagcacaagcagcatcagcagcatcaacaacagcatcaacagcagcatcaacagcagcatcaacagcttcagcagcttcagttcaaccctccacaaagccagccactcctggaaggcggaagcggtttccgtggaagcggaatggagatgacctcaattagagaagagatgggcgaacatcaacagggcttaagtgtatagtactagagggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtccatactagatgtctcacggacagcctccccctggatccagcatgtatggctttggagccatggggatgggatccggtatggaaactggcatgggaaccggcatgggaaccggcatgtcagcctcgcaggtgcgcagcagcagcaagcagcagcaagcagcattttcttttccgtcagatttttttttctctcctttttttcaagagagaaaaaaaaaaaaattgtttctctttgctggacgaaaatggtgagcaagctaacgattcgccgattgattggctttcaagctgtagatgacttccgacccacaggacatgatgtcgcttctggatacttcagtgtttcctgggtttgacggaatgtcgatgagtctcgatgttggcgattccatgtcaaaccccttcaccccggtctcggtcccgccacccctcccagcaggtaatgcagggcccagtcatgttggggtgtgcggcggtcacggggcacccgaccagctattttcccccgacgaccttattgcgaccagcatgtccagcgccggaccgatgatcgccacgcctaccaccaccacctccggaccttcgggcggcccttcgtcgggcggcgggagcaccctgaccgaatttaccaagcgccgcaattggcccgccaaggttgtcgaagagctccaggactgggaacacattctggatgccaacggacggatcaagcatgtttcgcccagcgtagagcccttgaccggctataagccgcccgaaatcattgacttgttcctcagagacctgatccacccggatgacgtcggggtcttcaccgcggagttgaacgaagccattgcgacgggatcccaattgcgactgttctaccggttccggaaaaaggatggaaactggaccattttcgagactgttggtcatgcgcacatcgccgccgccaagtttgcacccaatccacagaaccagtcgcctttctgccaggccgttttcatgatggctcgaccctacccgaccaagaacgcgggtctgttggattcctttctggaacacaagattgagaacgagagactgaagcgcaggattgccgagctgcgcagagaggagcaggaggagcaggaagagtcgcagaggacatggcgaatgagccaggagggacgatctgatgttacgccgtccgacgataccgcgacccagatgggaatgacgcccttctacattcccatgaatgcccaagctgacgttatgatgccaccacccagccagccggcgtcgtcgctcaacattgcgctgacgcgggagaacctggaaggcattgccggaagccggccggattctatacgcgaaaagatgttgcgttacgagggcaaccatgcagacacgattgaaatgcttacggggctcaagtaccaggagggtgaaagaagtcatggtatcacaacgggtaatgccagcccgacgctcattaagggtgatgcgggaatcgcgattcccctagaccgagatccccggacgggagagaagaagaagaagatcaaggttgcagaggaatacgtgtgcaccgactgcggtaattaccccttctccatattatataacccgctcccaaaatagcttcttgatgactaccagttgctgacaaggatgctgaaacaggtacgctcgattcccccgaatggcgaaaaggccctagtggacccaagacactatgtaatgcctgcggtcttcgctgggcaaagaaagagaaaaagaagaacgccaacaacaacaataacggcggaggtataggcggtcacaacgacatccatacacccatgggcgatcatatgggatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z