BBa_K1935019 1 BBa_K1935019 DNMT3a/DNMT3l 2016-10-13T11:00:00Z 2016-10-14T09:43:35Z Dnmt3a http://www.uniprot.org/uniprot/Q9Y6K1 Dnmt3l http://www.uniprot.org/uniprot/Q9UJW3 Dnmt3a: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Dnmt3l: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. In vertebrates, Transcription Start Sites (TSS) are divided into two categories: On the one hand, there are specific consensus sequences, such as the TATA box for example, that are recognized by transcription factors that recruit in turn RNA polymerases. On the other hand, there are about 1 kb large DNA sequences with a high content of cytosine and guanine dinucleotides. This last group is named ???CpG promoters??? or ???CGIs??? (CpG islands). It is the one which is targeted by DNA methyltransferases. Indeed, these regions are typically non-methylated, compared to the rest of the genome. In addition to their CG abundance, CGIs show a particular histone modification signature: they are enriched in H3K4me3.This characteristic has consequences: it maintains DNMTs inactive. Indeed, DNA methyltransferases get activated only when bound to H3K27me3 via their ADD domain. That may explain why CGI are most of the time unmethylated. false false _2402_ 25368 25368 9 false in progress false Emilie Gounin annotation2506271 1 Dnmt3a catalytic core range2506271 1 21 903 annotation2506279 1 Dnmt3l range2506279 1 985 1600 annotation2506530 1 linker range2506530 1 904 984 BBa_K1935019_sequence 1 aaccatgaccaggaatttgaccccccaaaggtttacccacctgtgccagctgagaagaggaagcccatccgcgtgctgtctctctttgatgggattgctacagggctcctggtgctgaaggacctgggcatccaagtggaccgctacattgcctccgaggtgtgtgaggactccatcacggtgggcatggtgcggcaccagggaaagatcatgtacgtcggggacgtccgcagcgtcacacagaagcatatccaggagtggggcccattcgacctggtgattggaggcagtccctgcaatgacctctccattgtcaaccctgcccgcaagggactttatgagggtactggccgcctcttctttgagttctaccgcctcctgcatgatgcgcggcccaaggagggagatgatcgccccttcttctggctctttgagaatgtggtggccatgggcgttagtgacaagagggacatctcgcgatttcttgagtctaaccccgtgatgattgacgccaaagaagtgtctgctgcacacagggcccgttacttctggggtaaccttcctggcatgaacaggcctttggcatccactgtgaatgataagctggagctgcaagagtgtctggagcacggcagaatagccaagttcagcaaagtgaggaccattaccaccaggtcaaactctataaagcagggcaaagaccagcatttccccgtcttcatgaacgagaaggaggacatcctgtggtgcactgaaatggaaagggtgtttggcttccccgtccactacacagacgtctccaacatgagccgcttggcgaggcagagactgctgggccgatcgtggagcgtgccggtcatccgccacctcttcgctccgctgaaggaatattttgcttgtgtgagcagcggcaacagcaacgccaacagccgcggccccagcttcagcagcggcctggtgcccctgagcctgcgcggcagccacatgggccctatggagatatacaagacagtgtctgcatggaagagacagccagtgcgggtactgagcctttttagaaatattgataaagtactaaagagtttgggctttttggaaagcggttctggttctgggggaggaacgctgaagtacgtggaagatgtcacaaatgtcgtgaggagagacgtggagaaatggggcccctttgacctggtgtacggctcgacgcagcccctaggcagctcttgtgatcgctgtcccggctggtacatgttccagttccaccgaatcctccagtatgcgctgcctcgccaggagagtcagcggcccttcttctggatattcatggacaatctgctgctgactgaggatgaccaagagacaactacccgcttccttcagacagaggctgtgaccctccaggatgtccgtggcagagactaccagaatgctatgcgggtgtggagcaacattccagggctgaagagcaagcatgcgcccctgaccccaaaggaagaagagtatctgcaagcccaagtcagaagcaggagcaagctggacgccccgaaagttgacctcctggtgaagaactgccttctcccgctgagagagtacttcaagtatttttctcaaaactcacttcctctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z