BBa_K1935020 1 BBa_K1935020 General control protein GCN4 2016-10-13T11:00:00Z 2016-10-14T10:00:01Z http://www.uniprot.org/uniprot/P03069 GCN4 is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'. It's naturally expressed in nuclei of Saccharomyces cerevisiae. Tanenbaum used it in a protein tagging system for signal amplification named Sun-tag (tagging system SUperNova) where a dCas9 fused to several GCN4 appeals paratopes fused to GFP. We also plan to use a dead Cas9 completed with several GCN4 epitopes to amplify DNA methylase activity. false false _2402_ 25368 25368 9 false in progress false Emilie Gounin annotation2506720 1 GCN4 (4) range2506720 1 217 267 annotation2506740 1 GCN4 (9) range2506740 1 577 627 annotation2506731 1 GCN4 (7) range2506731 1 433 483 annotation2506717 1 GCN4 (2) range2506717 1 73 123 annotation2506712 1 GCN4 (1) range2506712 1 1 51 annotation2506718 1 GCN4 (3) range2506718 1 145 195 annotation2506723 1 GCN4 (6) range2506723 1 361 411 annotation2506722 1 GCN4 (5) range2506722 1 289 339 annotation2506734 1 GCN4 (8) range2506734 1 505 555 annotation2506742 1 GCN4 (10) range2506742 1 649 699 BBa_K1935020_sequence 1 gaagaacttttgagcaagaattatcatcttgagaacgaagtggctcgtcttaagaaaggttctggcagtggagaagaactgctttcaaagaattaccacctggaaaatgaggtagctagactgaaaaaggggagcggaagtggggaggagttgctgagcaaaaattatcatttggagaacgaagtagcacgactaaagaaagggtccggatcgggtgaggagttactctcgaaaaattatcatctcgaaaacgaagtggctcggctaaaaaagggcagtggttctggagaagagctattatctaaaaactaccacctcgaaaatgaggtggcacgcttaaaaaagggaagtggcagtggtgaagagctactatccaagaattatcatcttgagaacgaggtagcgcgtttgaagaagggttccggctcaggagaggaactgctctcgaagaactatcatcttgaaaatgaggtcgctcgattaaaaaagggatcgggcagtggtgaggaactactttcaaagaattaccacctcgaaaacgaagtagctcgattaaagaaaggttcagggtcgggtgaagaattactgagtaaaaattatcatctggaaaatgaggtagcgagactaaaaaaggggagtggttctggcgaggaattgctatcgaaaaattatcatcttgagaacgaagttgctaggctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z