BBa_K1935020
1
BBa_K1935020
General control protein GCN4
2016-10-13T11:00:00Z
2016-10-14T10:00:01Z
http://www.uniprot.org/uniprot/P03069
GCN4 is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'. It's naturally expressed in nuclei of Saccharomyces cerevisiae.
Tanenbaum used it in a protein tagging system for signal amplification named Sun-tag (tagging system SUperNova) where a dCas9 fused to several GCN4 appeals paratopes fused to GFP.
We also plan to use a dead Cas9 completed with several GCN4 epitopes to amplify DNA methylase activity.
false
false
_2402_
25368
25368
9
false
in progress
false
Emilie Gounin
annotation2506720
1
GCN4 (4)
range2506720
1
217
267
annotation2506740
1
GCN4 (9)
range2506740
1
577
627
annotation2506731
1
GCN4 (7)
range2506731
1
433
483
annotation2506717
1
GCN4 (2)
range2506717
1
73
123
annotation2506712
1
GCN4 (1)
range2506712
1
1
51
annotation2506718
1
GCN4 (3)
range2506718
1
145
195
annotation2506723
1
GCN4 (6)
range2506723
1
361
411
annotation2506722
1
GCN4 (5)
range2506722
1
289
339
annotation2506734
1
GCN4 (8)
range2506734
1
505
555
annotation2506742
1
GCN4 (10)
range2506742
1
649
699
BBa_K1935020_sequence
1
gaagaacttttgagcaagaattatcatcttgagaacgaagtggctcgtcttaagaaaggttctggcagtggagaagaactgctttcaaagaattaccacctggaaaatgaggtagctagactgaaaaaggggagcggaagtggggaggagttgctgagcaaaaattatcatttggagaacgaagtagcacgactaaagaaagggtccggatcgggtgaggagttactctcgaaaaattatcatctcgaaaacgaagtggctcggctaaaaaagggcagtggttctggagaagagctattatctaaaaactaccacctcgaaaatgaggtggcacgcttaaaaaagggaagtggcagtggtgaagagctactatccaagaattatcatcttgagaacgaggtagcgcgtttgaagaagggttccggctcaggagaggaactgctctcgaagaactatcatcttgaaaatgaggtcgctcgattaaaaaagggatcgggcagtggtgaggaactactttcaaagaattaccacctcgaaaacgaagtagctcgattaaagaaaggttcagggtcgggtgaagaattactgagtaaaaattatcatctggaaaatgaggtagcgagactaaaaaaggggagtggttctggcgaggaattgctatcgaaaaattatcatcttgagaacgaagttgctaggctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z