BBa_K1935022
1
BBa_K1935022
SET-16 promoter in Caenorhabditis elegans
2016-10-13T11:00:00Z
2016-10-14T10:26:37Z
http://www.wormbase.org/species/c_elegans/gene/WBGene00011729#0-9g-3
Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm???s promoter able to be regulated as mammalian CpG island.
pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands.
Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene.
That???s why we chose it as a target to test epiCRISPR whithout killing the worm.
false
false
_2402_
25368
25368
9
false
in progress
false
Emilie Gounin
BBa_K1935022_sequence
1
gacgtcttctgaaattcatttaagtctttaaaaacgcacacacgaaagctcgaaaatacatatttgcaaatcaaattatttatttaatttttttccaactctcagtccgcaatctcgcgtggcattcggtgtattggcacagtgccagagagacgcagtgcggcagagatgcgtagccgccccgttaacatggcaaggggctctacgcagcgcgtagagacgcagacatgtaacgcttggcaagatgccaaacaaatcgatacgcccttttcaacagccgttagaggagaatgggcggca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z