BBa_K193601
1
placIQ RBS
Constitutive Promoter (placIQ ) + RBS + melA on Low copy vector(pSB6A1)
2009-10-13T11:00:00Z
2015-05-08T01:11:16Z
We used I14032, B0030 and K193600.
This composite part contains pLacIQ(BBa_I14032) and RBS(B0030) and melA on low copy vector(PSB6A1).
false
true
_288_
0
5245
9
It's complicated
true
We used biobrick standard assembly.
false
Kazuaki Amikura
component2072752
1
BBa_B0030
component2072754
1
BBa_K193600
component2072750
1
BBa_I14032
annotation2072754
1
BBa_K193600
range2072754
1
67
1896
annotation2072750
1
BBa_I14032
range2072750
1
1
37
annotation2072752
1
BBa_B0030
range2072752
1
46
60
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K193600
1
BBa_K193600
melA gene
2009-10-13T11:00:00Z
2015-05-08T01:11:16Z
The gene sequence was obtained from GENEART.
melA is coding sequence of tyrosinase. Tyrosinase is enzyme which converts from tyrosine to dopaquinone. Dopaquinone is intermediate product of melanin biosynthesis pathway.
false
false
_288_
0
5245
9
Not in stock
false
We added restriction enzyme sites (Prefix-Suffix) to the upstream and down stream of the ORF of melA.
false
Kazuaki Amikura
BBa_I14032
1
P(Lac) IQ
promoter P(Lac) IQ
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pMAL-p2X
Released HQ 2013
Constitutive Promoter, High Transcription
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1028344
1
-10
range1028344
1
26
31
annotation1028342
1
P(Lac) IQ
range1028342
1
1
37
annotation1028343
1
-35
range1028343
1
3
8
BBa_I14032_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K193600_sequence
1
atggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa
BBa_K193601_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z