BBa_K193602 1 placIQ RBS placIQ RBS melA on pSB4A5 2009-10-13T11:00:00Z 2015-05-08T01:11:16Z We used I14032, B0030 and K193600. This composite part contains pLacIQ(BBa_I14032) and RBS(B0030) and melA on low copy vector(PSB4A5). false true _288_ 0 5245 9 It's complicated true We used biobrick standard assembly. false Kazuaki Amikura component2072758 1 BBa_I14032 component2072760 1 BBa_B0030 component2072762 1 BBa_K193600 annotation2072758 1 BBa_I14032 range2072758 1 1 37 annotation2072762 1 BBa_K193600 range2072762 1 67 1896 annotation2072760 1 BBa_B0030 range2072760 1 46 60 BBa_K193600 1 BBa_K193600 melA gene 2009-10-13T11:00:00Z 2015-05-08T01:11:16Z The gene sequence was obtained from GENEART. melA is coding sequence of tyrosinase. Tyrosinase is enzyme which converts from tyrosine to dopaquinone. Dopaquinone is intermediate product of melanin biosynthesis pathway. false false _288_ 0 5245 9 Not in stock false We added restriction enzyme sites (Prefix-Suffix) to the upstream and down stream of the ORF of melA. false Kazuaki Amikura BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028343 1 -35 range1028343 1 3 8 annotation1028344 1 -10 range1028344 1 26 31 annotation1028342 1 P(Lac) IQ range1028342 1 1 37 BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_B0030_sequence 1 attaaagaggagaaa BBa_K193602_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa BBa_K193600_sequence 1 atggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z