LOCUS BBa_K1937004 6857 bp DNA linear 22-Nov-2024 DEFINITION pNagA-RFP in pSBBs0K-mini plasmid ACCESSION BBa_K1937004 VERSION BBa_K1937004.1 FEATURES Location/Qualifiers promoter 1..200 /label=Promoter NagP+RBS CDS 207..912 /label=RFP misc_feature 913..992 /label=Terminator misc_feature 1..992 /label=BBa_K1937003 CDS 6202..6600 /label=Bleomycin resistance (B. subtilis) CDS 2564..3223 /label=Ampicillin resistance (E. coli) misc_feature 1784..2466 /label=ColE1 origin (E. coli) CDS 5209..5979 /label=Kanamycin resistance (B. subtilis) misc_feature 1257..1278 /label=Prefix misc_feature 1285..1305 /label=Suffixe misc_feature 4045..5049 /label=repU (Bacillus subtilis origin) primer_bind 3546..3565 /label=Verification forward (VF2) primer binding site primer_bind 1685..1704 /label=Verification reverse (VR) primer binding site misc_feature 1001..6857 /label=BBa_K1937001 ORIGIN 1 ttgtgcgaac ctttgccacg atatgttcct cctgttccgg gctgccccga gcttgctcac 61 aatactttca ttttatcact ttcgggcttg aacctaaaac agattttata aaagggggga 121 aaacacctca gctggtatag atcactaatc tgaaaaagag taaaataaag gtattcaaat 181 tccagaaagg cggatcatct gctagcatgg cttcctccga agacgttatc aaagagttca 241 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 301 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 361 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 421 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 481 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct 541 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 601 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 661 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 721 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 781 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg 841 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct 901 agtgtagatc gcccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 961 gttttatctg ttgtttgtcg gtgaacgctc tctactagag atctattatt aatctgttca 1021 gcaatcgggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt 1081 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct 1141 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa 1201 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttgaat 1261 tcgcggccgc ttctagaggc tagctactag tagcggccgc tgcaggcgca acgcaattaa 1321 tgtgagttag gatcctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 1381 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 1441 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 1501 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 1561 accatatgcg gtgtgaaata ccgcacagat gcgtaaggaa aaaataccgc atcaggcgct 1621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 1681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 1741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 1801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 1861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 1921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 1981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 2161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 2221 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 2281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 2341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 2401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 2461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 2521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 2581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 2641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 2701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 2761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 2821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgccg 2881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 2941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 3001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 3061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 3121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 3181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 3241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 3301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 3361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 3421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 3481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 3541 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 3601 gtatcacgag gccctttcgt cttcaagagt tccttaagga acgtacagac ggcttaaaag 3661 cctttaaaaa cgtttttaag gggtttgtag acaaggtaaa ggataaaaca gcacaattcc 3721 aagaaaaaca cgatttagaa cctaaaaaga acgaatttga actaactcat aaccgagagg 3781 taaaaaaaga acgaagtcga gatcagggaa tgagtttata aaataaaaaa agcacctgaa 3841 aaggtgtctt tttttgatgg ttttgaactt gttctttctt atcttgatac atatagaaat 3901 aacgtcattt ttattttagt tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt 3961 aaagtattga aaacccttaa aattggttgc acagaaaaac cccatctgtt aaagttataa 4021 gtgactaaac aaataactaa atagatgggg gtttctttta atattatgtg tcctaatagt 4081 agcatttatt cagatgaaaa atcaagggtt ttagtggaca agacaaaaag tggaaaagtg 4141 agaccatgga gagaaaagaa aatcgctaat gttgattact ttgaacttct gcatattctt 4201 gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa tattagagta taaacaaaat 4261 cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag gctttgtcca 4321 atgtgcaact ggaggagagc aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa 4381 gttattaaac aaaagccaac agttcgttgg ttgtttctca cattaacagt taaaaatgtt 4441 tatgatggcg aagaattaaa taagagtttg tcagatatgg ctcaaggatt tcgccgaatg 4501 atgcaatata aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca 4561 ataaataata aagataattc ttataatcag cacatgcatg tattggtatg tgtggaacca 4621 acttatttta agaatacaga aaactacgtg aatcaaaaac aatggattca attttggaaa 4681 aaggcaatga aattagacta tgatccaaat gtaaaagttc aaatgattcg accgaaaaat 4741 aaatataaat cggatataca atcggcaatt gacgaaactg caaaatatcc tgtaaaggat 4801 acggatttta tgaccgatga tgaagaaaag aatttgaaac gtttgtctga tttggaggaa 4861 ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat acataaaaaa 4921 ttaaaccttg atgacacaga agaaggcgat ttgattcata cagatgatga cgaaaaagcc 4981 gatgaagatg gattttctat tattgcaatg tggaattggg aacggaaaaa ttattttatt 5041 aaagagtagt tcaacaaacg ggccagtttg ttgaagatta gatgctataa ttgttattaa 5101 aaggattgaa ggatgcttag gaagacgagt tattaatagc tgaataagaa cggtgctctc 5161 caaatattct tatttagaaa agcaaatcta aaattatctg aaaagggaat gagaatagtg 5221 aatggaccaa taataatgac tagagaagaa agaatgaaga ttgttcatga aattaaggaa 5281 cgaatattgg ataaatatgg ggatgatgtt aaggctattg gtgtttatgg ctctcttggt 5341 cgtcagactg atgggcccta ttcggatatt gagatgatgt gtgtcatgtc aacagaggaa 5401 gcagagttca gccatgaatg gacaaccggt gagtggaagg tggaagtgaa ttttgatagc 5461 gaagagattc tactagatta tgcatctcag gtggaatcag attggccgct tacacatggt 5521 caatttttct ctattttgcc gatttatgat tcaggtggat acttagagaa agtgtatcaa 5581 actgctaaat cggtagaagc ccaaacgttc cacgatgcga tttgtgccct tatcgtagaa 5641 gagctgtttg aatatgcagg caaatggcgt aatattcgtg tgcaaggacc gacaacattt 5701 ctaccatcct tgactgtaca ggtagcaatg gcaggtgcca tgttgattgg tctgcatcat 5761 cgcatctgtt atacgacgag cgcttcggtc ttaactgaag cagttaagca atcagatctt 5821 ccttcaggtt atgaccatct gtgccagttc gtaatgtctg gtcaactttc cgactctgag 5881 aaacttctgg aatcgctaga gaatttctgg aatgggattc aggagtggac agaacgacac 5941 ggatatatag tggatgtgtc aaaacgcata ccattttgaa cgatgacctc taataattgt 6001 taatcatgtt ggttacgtat ttattaactt ctcctagtat tagtaattat catggctgtc 6061 atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt aataagaaaa 6121 aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat tagaaaatga 6181 aaggggattt tatgcgtgag aatgttacag tctatcccgg cattgccagt cggggatatt 6241 aaaaagagta taggttttta ttgcgataaa ctaggtttca ctttggttca ccatgaagat 6301 ggattcgcag ttctaatgtg taatgaggtt cggattcatc tatgggaggc aagtgatgaa 6361 ggctggcgct ctcgtagtaa tgattcaccg gtttgtacag gtgcggagtc gtttattgct 6421 ggtactgcta gttgccgcat tgaagtagag ggaattgatg aattatatca acatattaag 6481 cctttgggca ttttgcaccc caatacatca ttaaaagatc agtggtggga tgaacgagac 6541 tttgcagtaa ttgatcccga caacaatttg attagctttt ttcaacaaat aaaaagctat 6601 aatctattat taatctgttc agcaatcggg cgcgattgct gaataaaaga tacgagagac 6661 ctctcttgta tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac 6721 aataaaaatt ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat 6781 aaaaattggc aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac 6841 ctgtcccttg ctgattt //