LOCUS BBa_K1937006 6878 bp DNA linear 22-Nov-2024 DEFINITION pNagP-RFP in pSBBs0K-mini plasmid ACCESSION BBa_K1937006 VERSION BBa_K1937006.1 FEATURES Location/Qualifiers promoter 1..200 /label=Promoter NagP+RBS CDS 207..912 /label=RFP misc_feature 913..992 /label=Terminator misc_feature 1..1013 /label=BBa_K1937005 CDS 6223..6621 /label=Bleomycin resistance (B. subtilis) CDS 2585..3244 /label=Ampicillin resistance (E. coli) misc_feature 1805..2487 /label=ColE1 origin (E. coli) CDS 5230..6000 /label=Kanamycin resistance (B. subtilis) misc_feature 1278..1299 /label=Prefix misc_feature 1306..1326 /label=Suffixe misc_feature 4066..5070 /label=repU (Bacillus subtilis origin) primer_bind 3567..3586 /label=Verification forward (VF2) primer binding site primer_bind 1706..1725 /label=Verification reverse (VR) primer binding site misc_feature 1022..6878 /label=BBa_K1937001 ORIGIN 1 gaattcgcgg ccgcttctag atctaattct tgatcgtttg ttacaatcat ctgtcattcc 61 cgctttcttt ttaaataatg tagattaagc ttaccacaac tgtcttaaaa ataggaaaca 121 cacggacctg ggaaaaaaga aatacccccg ggaaaattgg tatagatcac tagatatctt 181 atatggtata tttgaaaaaa aagggtatga gggggatggg tgctagcatg gcttcctccg 241 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc 301 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta 361 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt 421 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac 481 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg 541 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc 601 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag 661 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc 721 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa 781 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca 841 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg 901 cttaataacg ctgatagtgc tagtgtagat cgcccaggca tcaaataaaa cgaaaggctc 961 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1021 gatctattat taatctgttc agcaatcggg cgcgattgct gaataaaaga tacgagagac 1081 ctctcttgta tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac 1141 aataaaaatt ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat 1201 aaaaattggc aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac 1261 ctgtcccttg ctgatttgaa ttcgcggccg cttctagagg ctagctacta gtagcggccg 1321 ctgcaggcgc aacgcaatta atgtgagtta ggatcctgcc tcgcgcgttt cggtgatgac 1381 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 1441 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 1501 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 1561 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 1621 aaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 1681 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 1741 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 1801 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 1861 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 1921 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 1981 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 2041 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 2101 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 2161 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 2221 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 2281 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 2341 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 2401 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 2461 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 2521 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 2581 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 2641 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 2701 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 2761 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 2821 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 2881 acgttgttgc cattgctgcc ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 2941 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 3001 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 3061 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 3121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 3181 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 3241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 3301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 3481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 3541 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 3601 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagag ttccttaagg 3661 aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa ggggtttgta gacaaggtaa 3721 aggataaaac agcacaattc caagaaaaac acgatttaga acctaaaaag aacgaatttg 3781 aactaactca taaccgagag gtaaaaaaag aacgaagtcg agatcaggga atgagtttat 3841 aaaataaaaa aagcacctga aaaggtgtct ttttttgatg gttttgaact tgttctttct 3901 tatcttgata catatagaaa taacgtcatt tttattttag ttgctgaaag gtgcgttgaa 3961 gtgttggtat gtatgtgttt taaagtattg aaaaccctta aaattggttg cacagaaaaa 4021 ccccatctgt taaagttata agtgactaaa caaataacta aatagatggg ggtttctttt 4081 aatattatgt gtcctaatag tagcatttat tcagatgaaa aatcaagggt tttagtggac 4141 aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga aaatcgctaa tgttgattac 4201 tttgaacttc tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga ttgtgctgaa 4261 atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg agtgtggttt 4321 tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag caatgaaaca tggcattcag 4381 tcacaaaagg ttgttgctga agttattaaa caaaagccaa cagttcgttg gttgtttctc 4441 acattaacag ttaaaaatgt ttatgatggc gaagaattaa ataagagttt gtcagatatg 4501 gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct tgttggtttt 4561 atgcgtgcaa cggaagtgac aataaataat aaagataatt cttataatca gcacatgcat 4621 gtattggtat gtgtggaacc aacttatttt aagaatacag aaaactacgt gaatcaaaaa 4681 caatggattc aattttggaa aaaggcaatg aaattagact atgatccaaa tgtaaaagtt 4741 caaatgattc gaccgaaaaa taaatataaa tcggatatac aatcggcaat tgacgaaact 4801 gcaaaatatc ctgtaaagga tacggatttt atgaccgatg atgaagaaaa gaatttgaaa 4861 cgtttgtctg atttggagga aggtttacac cgtaaaaggt taatctccta tggtggtttg 4921 ttaaaagaaa tacataaaaa attaaacctt gatgacacag aagaaggcga tttgattcat 4981 acagatgatg acgaaaaagc cgatgaagat ggattttcta ttattgcaat gtggaattgg 5041 gaacggaaaa attattttat taaagagtag ttcaacaaac gggccagttt gttgaagatt 5101 agatgctata attgttatta aaaggattga aggatgctta ggaagacgag ttattaatag 5161 ctgaataaga acggtgctct ccaaatattc ttatttagaa aagcaaatct aaaattatct 5221 gaaaagggaa tgagaatagt gaatggacca ataataatga ctagagaaga aagaatgaag 5281 attgttcatg aaattaagga acgaatattg gataaatatg gggatgatgt taaggctatt 5341 ggtgtttatg gctctcttgg tcgtcagact gatgggccct attcggatat tgagatgatg 5401 tgtgtcatgt caacagagga agcagagttc agccatgaat ggacaaccgg tgagtggaag 5461 gtggaagtga attttgatag cgaagagatt ctactagatt atgcatctca ggtggaatca 5521 gattggccgc ttacacatgg tcaatttttc tctattttgc cgatttatga ttcaggtgga 5581 tacttagaga aagtgtatca aactgctaaa tcggtagaag cccaaacgtt ccacgatgcg 5641 atttgtgccc ttatcgtaga agagctgttt gaatatgcag gcaaatggcg taatattcgt 5701 gtgcaaggac cgacaacatt tctaccatcc ttgactgtac aggtagcaat ggcaggtgcc 5761 atgttgattg gtctgcatca tcgcatctgt tatacgacga gcgcttcggt cttaactgaa 5821 gcagttaagc aatcagatct tccttcaggt tatgaccatc tgtgccagtt cgtaatgtct 5881 ggtcaacttt ccgactctga gaaacttctg gaatcgctag agaatttctg gaatgggatt 5941 caggagtgga cagaacgaca cggatatata gtggatgtgt caaaacgcat accattttga 6001 acgatgacct ctaataattg ttaatcatgt tggttacgta tttattaact tctcctagta 6061 ttagtaatta tcatggctgt catggcgcat taacggaata aagggtgtgc ttaaatcggg 6121 ccattttgcg taataagaaa aaggattaat tatgagcgaa ttgaattaat aataaggtaa 6181 tagatttaca ttagaaaatg aaaggggatt ttatgcgtga gaatgttaca gtctatcccg 6241 gcattgccag tcggggatat taaaaagagt ataggttttt attgcgataa actaggtttc 6301 actttggttc accatgaaga tggattcgca gttctaatgt gtaatgaggt tcggattcat 6361 ctatgggagg caagtgatga aggctggcgc tctcgtagta atgattcacc ggtttgtaca 6421 ggtgcggagt cgtttattgc tggtactgct agttgccgca ttgaagtaga gggaattgat 6481 gaattatatc aacatattaa gcctttgggc attttgcacc ccaatacatc attaaaagat 6541 cagtggtggg atgaacgaga ctttgcagta attgatcccg acaacaattt gattagcttt 6601 tttcaacaaa taaaaagcta taatctatta ttaatctgtt cagcaatcgg gcgcgattgc 6661 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt 6721 tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa 6781 gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt 6841 gatcttctca aaaaatacta cctgtccctt gctgattt //