LOCUS BBa_K1937008 6828 bp DNA linear 14-Nov-2024 DEFINITION AF-A antifungal operon in pSBBs0K-mini ACCESSION BBa_K1937008 VERSION BBa_K1937008.1 FEATURES Location/Qualifiers promoter 1..237 /label=Pveg RBS 238..249 /label=RBS Metchnikowin CDS 253..375 /label=amyE for cutted Metchnikowin CDS 376..453 /label=Cutted Metchnikowin RBS 454..465 /label=RBS D4E1 CDS 466..588 /label=AmyE for D4E1 CDS 589..648 /label=D4E1 misc_feature 1..963 /label=BBa_K1937007 CDS 6173..6571 /label=Bleomycin resistance (B. subtilis) CDS 2535..3194 /label=Ampicillin resistance (E. coli) misc_feature 1755..2437 /label=ColE1 origin (E. coli) CDS 5180..5950 /label=Kanamycin resistance (B. subtilis) misc_feature 1228..1249 /label=Prefix misc_feature 1256..1276 /label=Suffixe misc_feature 4016..5020 /label=repU (Bacillus subtilis origin) primer_bind 3517..3536 /label=Verification forward (VF2) primer binding site primer_bind 1656..1675 /label=Verification reverse (VR) primer binding site misc_feature 972..6828 /label=BBa_K1937001 ORIGIN 1 ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac ctgcatagga 61 gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac ttgcggaaca 121 taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt 181 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagtaaa 241 ggtggtgaag ctagcatgtt tgctaagaga ttcaaaacat ccttactccc tttgttcgcg 301 ggatttttgc tgctgtttca cctcgtactg gccggtccgg cagcagccag tgcagaaacc 361 gcgaataagt ctaatgagca tagacatcaa ggcccgattt ttgatacaag accgtcaccg 421 tttaatccga atcaaccgag accgggcccg atttataaag gtggtgaaat gttcgctaaa 481 agattcaaaa ccagcctgct gcctttattt gccggattcc tccttctgtt ccacttagtg 541 ctcgcgggtc cggcagcagc aagcgccgaa acggcaaata agtcaaatga gtcaagttac 601 gggcgaaaat caaagtacgg ctgcgggcga agatcaagct atgaccaggc accgcgggtc 661 gactgttatc cggctatatt gcaggagcga ttatgaaaca agaagttatc ctggtactcg 721 actgtggcgc gaccaatgtc agggccatcg cggttaatcg gcagggcaaa attgttgccc 781 gcgcctcaac gcctaatgcc agcgatatcg cgatggaaaa caacacctgg caccagtggt 841 ctttagacgc cattttgcaa cgctttgctg attgctgtcg gcaaatcaat agtgaactga 901 ctgaatgcca catccgaggt atcgccgtca ccacctttgg tgtggatggc gctctggtag 961 atatactaga gatctattat taatctgttc agcaatcggg cgcgattgct gaataaaaga 1021 tacgagagac ctctcttgta tcttttttat tttgagtggt tttgtccgtt acactagaaa 1081 accgaaagac aataaaaatt ttattcttgc tgagtctggc tttcggtaag ctagacaaaa 1141 cggacaaaat aaaaattggc aagggtttaa aggtggagat tttttgagtg atcttctcaa 1201 aaaatactac ctgtcccttg ctgatttgaa ttcgcggccg cttctagagg ctagctacta 1261 gtagcggccg ctgcaggcgc aacgcaatta atgtgagtta ggatcctgcc tcgcgcgttt 1321 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 1381 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 1441 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 1501 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 1561 tgcgtaagga aaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 1621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 1681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 1741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 1801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 1861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 1921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 1981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 2041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 2101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 2161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 2221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 2281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 2341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 2401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 2461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 2521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 2581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 2641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 2701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 2761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 2821 agtttgcgca acgttgttgc cattgctgcc ggcatcgtgg tgtcacgctc gtcgtttggt 2881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 2941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 3001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 3061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 3121 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 3181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 3241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 3301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 3361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 3421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 3481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 3541 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagag 3601 ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa ggggtttgta 3661 gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga acctaaaaag 3721 aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg agatcaggga 3781 atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg gttttgaact 3841 tgttctttct tatcttgata catatagaaa taacgtcatt tttattttag ttgctgaaag 3901 gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta aaattggttg 3961 cacagaaaaa ccccatctgt taaagttata agtgactaaa caaataacta aatagatggg 4021 ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa aatcaagggt 4081 tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga aaatcgctaa 4141 tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga 4201 ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg 4261 agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag caatgaaaca 4321 tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa cagttcgttg 4381 gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa ataagagttt 4441 gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct 4501 tgttggtttt atgcgtgcaa cggaagtgac aataaataat aaagataatt cttataatca 4561 gcacatgcat gtattggtat gtgtggaacc aacttatttt aagaatacag aaaactacgt 4621 gaatcaaaaa caatggattc aattttggaa aaaggcaatg aaattagact atgatccaaa 4681 tgtaaaagtt caaatgattc gaccgaaaaa taaatataaa tcggatatac aatcggcaat 4741 tgacgaaact gcaaaatatc ctgtaaagga tacggatttt atgaccgatg atgaagaaaa 4801 gaatttgaaa cgtttgtctg atttggagga aggtttacac cgtaaaaggt taatctccta 4861 tggtggtttg ttaaaagaaa tacataaaaa attaaacctt gatgacacag aagaaggcga 4921 tttgattcat acagatgatg acgaaaaagc cgatgaagat ggattttcta ttattgcaat 4981 gtggaattgg gaacggaaaa attattttat taaagagtag ttcaacaaac gggccagttt 5041 gttgaagatt agatgctata attgttatta aaaggattga aggatgctta ggaagacgag 5101 ttattaatag ctgaataaga acggtgctct ccaaatattc ttatttagaa aagcaaatct 5161 aaaattatct gaaaagggaa tgagaatagt gaatggacca ataataatga ctagagaaga 5221 aagaatgaag attgttcatg aaattaagga acgaatattg gataaatatg gggatgatgt 5281 taaggctatt ggtgtttatg gctctcttgg tcgtcagact gatgggccct attcggatat 5341 tgagatgatg tgtgtcatgt caacagagga agcagagttc agccatgaat ggacaaccgg 5401 tgagtggaag gtggaagtga attttgatag cgaagagatt ctactagatt atgcatctca 5461 ggtggaatca gattggccgc ttacacatgg tcaatttttc tctattttgc cgatttatga 5521 ttcaggtgga tacttagaga aagtgtatca aactgctaaa tcggtagaag cccaaacgtt 5581 ccacgatgcg atttgtgccc ttatcgtaga agagctgttt gaatatgcag gcaaatggcg 5641 taatattcgt gtgcaaggac cgacaacatt tctaccatcc ttgactgtac aggtagcaat 5701 ggcaggtgcc atgttgattg gtctgcatca tcgcatctgt tatacgacga gcgcttcggt 5761 cttaactgaa gcagttaagc aatcagatct tccttcaggt tatgaccatc tgtgccagtt 5821 cgtaatgtct ggtcaacttt ccgactctga gaaacttctg gaatcgctag agaatttctg 5881 gaatgggatt caggagtgga cagaacgaca cggatatata gtggatgtgt caaaacgcat 5941 accattttga acgatgacct ctaataattg ttaatcatgt tggttacgta tttattaact 6001 tctcctagta ttagtaatta tcatggctgt catggcgcat taacggaata aagggtgtgc 6061 ttaaatcggg ccattttgcg taataagaaa aaggattaat tatgagcgaa ttgaattaat 6121 aataaggtaa tagatttaca ttagaaaatg aaaggggatt ttatgcgtga gaatgttaca 6181 gtctatcccg gcattgccag tcggggatat taaaaagagt ataggttttt attgcgataa 6241 actaggtttc actttggttc accatgaaga tggattcgca gttctaatgt gtaatgaggt 6301 tcggattcat ctatgggagg caagtgatga aggctggcgc tctcgtagta atgattcacc 6361 ggtttgtaca ggtgcggagt cgtttattgc tggtactgct agttgccgca ttgaagtaga 6421 gggaattgat gaattatatc aacatattaa gcctttgggc attttgcacc ccaatacatc 6481 attaaaagat cagtggtggg atgaacgaga ctttgcagta attgatcccg acaacaattt 6541 gattagcttt tttcaacaaa taaaaagcta taatctatta ttaatctgtt cagcaatcgg 6601 gcgcgattgc tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg 6661 ttttgtccgt tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg 6721 ctttcggtaa gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga 6781 ttttttgagt gatcttctca aaaaatacta cctgtccctt gctgattt //