BBa_K1939002
1
BBa_K1939002
Mutant RBS with mCherry reporter gene
2016-10-06T11:00:00Z
2016-10-07T03:11:28Z
This is a mutant of the RBS region of the native strain, S001, of M. maripaludis
This composite part contains an RBS with a single point mutation (G to A transition) and the encoding region for the mCherry fluorescent reporter protein. This particular RBS mutation results in higher translational efficiency with mCherry fluorescence that is 137.96% of the native RBS.
false
false
_2406_
30108
30108
9
false
This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers.
false
Kyler Herrington
annotation2487516
1
mCherry
range2487516
1
196
906
annotation2487514
1
Promoter
range2487514
1
2
175
annotation2487517
1
BBa_K1383000
range2487517
1
1
906
annotation2487515
1
Native RBS
range2487515
1
185
190
BBa_K1939002_sequence
1
acgagataagaattactagataattcagctgatcgatcaaaatataacataaataacataggtttaaataatttaaaggcatattttatataaacaattgtaaaatattggcttatgaaatttgttaaaatttagctaataagctattgatattataattttatagataactaatactagagtgcagatagcgctatggtttcaaaaggagaagaagataatatggcaataataaaagaatttatgagatttaaagttcacatggaaggttcagttaatggtcacgaatttgagatagaaggtgaaggtgaaggtagaccatacgaaggtacacagacagcaaaattaaaagttacaaaaggtggtccattaccattcgcatgggatatattatcaccacagtttatgtacggttcaaaagcatacgttaaacacccagcagatataccagattacctcaaattatcatttccagaaggttttaaatgggaaagagttatgaattttgaagatggtggtgttgttacagttacacaggattcatcattacaggatggtgaatttatatacaaagttaaattaagaggtacaaattttccatcagatggtccagttatgcagaaaaaaacaatgggttgggaagcatcatcagaaagaatgtacccagaagatggtgcattaaaaggtgagataaaacagagattaaaattaaaagatggtggtcactacgatgcagaagttaaaacaacatacaaagcaaaaaaaccagttcagttaccaggtgcatacaacgttaatataaaattagatataacatcacacaatgaagattacacaatagttgaacagtacgaaagagcagaaggtagacactcaacaggtggtatggatgaattatacaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z