BBa_K1939003 1 BBa_K1939003 Mutant RBS with mCherry reporter gene 2016-10-06T11:00:00Z 2016-10-07T03:17:04Z This is a mutant of the RBS region of the native strain, S001, of M. maripaludis This composite part contains an RBS with a single point mutation (C to A transversion) and the encoding region for the mCherry fluorescent reporter protein. This particular RBS mutation results in lower translational efficiency with mCherry fluorescence that is 93.48% of the native RBS. false false _2406_ 30108 30108 9 false This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers. false Kyler Herrington annotation2487522 1 BBa_K1383000 range2487522 1 1 906 annotation2487519 1 Promoter range2487519 1 2 175 annotation2487521 1 mCherry range2487521 1 196 906 annotation2487520 1 Native RBS range2487520 1 185 190 BBa_K1939003_sequence 1 acgagataagaattactagataattcagctgatcgatcaaaatataacataaataacataggtttaaataatttaaaggcatattttatataaacaattgtaaaatattggcttatgaaatttgttaaaatttagctaataagctattgatattataattttatagataactaatactagagtgaaggtagcgctatggtttcaaaaggagaagaagataatatggcaataataaaagaatttatgagatttaaagttcacatggaaggttcagttaatggtcacgaatttgagatagaaggtgaaggtgaaggtagaccatacgaaggtacacagacagcaaaattaaaagttacaaaaggtggtccattaccattcgcatgggatatattatcaccacagtttatgtacggttcaaaagcatacgttaaacacccagcagatataccagattacctcaaattatcatttccagaaggttttaaatgggaaagagttatgaattttgaagatggtggtgttgttacagttacacaggattcatcattacaggatggtgaatttatatacaaagttaaattaagaggtacaaattttccatcagatggtccagttatgcagaaaaaaacaatgggttgggaagcatcatcagaaagaatgtacccagaagatggtgcattaaaaggtgagataaaacagagattaaaattaaaagatggtggtcactacgatgcagaagttaaaacaacatacaaagcaaaaaaaccagttcagttaccaggtgcatacaacgttaatataaaattagatataacatcacacaatgaagattacacaatagttgaacagtacgaaagagcagaaggtagacactcaacaggtggtatggatgaattatacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z