BBa_K1939004 1 BBa_K1939004 Mutant RBS with mCherry reporter gene 2016-10-06T11:00:00Z 2016-10-07T03:21:48Z This is a mutant of the RBS region of the native strain, S001, of M. maripaludis This composite part contains an RBS with a single point mutation (C to A transversion) and the encoding region for the mCherry fluorescent reporter protein. This particular RBS mutation results in lower translational efficiency with mCherry fluorescence that is 127.83% of the native RBS. false false _2406_ 30108 30108 9 false This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers. false Kyler Herrington annotation2487528 1 Native RBS range2487528 1 185 190 annotation2487527 1 Promoter range2487527 1 2 175 annotation2487530 1 BBa_K1383000 range2487530 1 1 906 annotation2487529 1 mCherry range2487529 1 196 906 BBa_K1939004_sequence 1 acgagataagaattactagataattcagctgatcgatcaaaatataacataaataacataggtttaaataatttaaaggcatattttatataaacaattgtaaaatattggcttatgaaatttgttaaaatttagctaataagctattgatattataattttatagataactaatactagagtgcaggtagcgatatggtttcaaaaggagaagaagataatatggcaataataaaagaatttatgagatttaaagttcacatggaaggttcagttaatggtcacgaatttgagatagaaggtgaaggtgaaggtagaccatacgaaggtacacagacagcaaaattaaaagttacaaaaggtggtccattaccattcgcatgggatatattatcaccacagtttatgtacggttcaaaagcatacgttaaacacccagcagatataccagattacctcaaattatcatttccagaaggttttaaatgggaaagagttatgaattttgaagatggtggtgttgttacagttacacaggattcatcattacaggatggtgaatttatatacaaagttaaattaagaggtacaaattttccatcagatggtccagttatgcagaaaaaaacaatgggttgggaagcatcatcagaaagaatgtacccagaagatggtgcattaaaaggtgagataaaacagagattaaaattaaaagatggtggtcactacgatgcagaagttaaaacaacatacaaagcaaaaaaaccagttcagttaccaggtgcatacaacgttaatataaaattagatataacatcacacaatgaagattacacaatagttgaacagtacgaaagagcagaaggtagacactcaacaggtggtatggatgaattatacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z