BBa_K1940001
type: ComponentDefinition
persistent identity: BBa K1940001
display id: BBa_K1940001
version: 1
was generated by: iGEM to SBOL conversion
description: microRNA-34c detector 1.0
created: 2016-10-10T11:00:00Z
modified: 2016-10-14T06:36:27Z
mutable provenance: composited
owned by: james
owned by: myers
mutable description: We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c ( BBa_K1940000) was insert after a constitutive promoter (BBa_J23106) which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP???s expression. Determining GFP???s fluorescence intensity differences will justify the content amount of microRNA-34c.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2407_
m user id: 30070
owner id: 30070
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: We had tested that anti-microRNA-34c be put in this position was most effective than be put after RBS or GFP.
star: false
creator: Yujia Liu
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_J23106
component: BBa_B0034
component: BBa_K1940000
component: BBa_B0015
component: BBa_E0040
sequence annotation: BBa_J23106
sequence annotation: BBa_K1940000
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1940001_sequence/1

BBa_J23106
type: Component
persistent identity: component2492854
display id: component2492854
version: 1
top level: BBa_K1940001
definition: BBa_J23106
access: public

BBa_B0034
type: Component
persistent identity: component2492864
display id: component2492864
version: 1
top level: BBa_K1940001
definition: BBa_B0034
access: public

BBa_K1940000
type: Component
persistent identity: component2492862
display id: component2492862
version: 1
top level: BBa_K1940001
definition: BBa_K1940000
access: public

BBa_B0015
type: Component
persistent identity: component2492873
display id: component2492873
version: 1
top level: BBa_K1940001
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2492866
display id: component2492866
version: 1
top level: BBa_K1940001
definition: GFP
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2492854
display id: annotation2492854
version: 1
top level: BBa_K1940001
location: https://synbiohub.org/public/igem/BBa_K1940001/annotation2492854/range2492854/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1940001/annotation2492854/range2492854/1
type: Range
persistent identity: range2492854
display id: range2492854
version: 1
top level: BBa_K1940001
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1940000
type: SequenceAnnotation
persistent identity: annotation2492862
display id: annotation2492862
version: 1
top level: BBa_K1940001
location: https://synbiohub.org/public/igem/BBa_K1940001/annotation2492862/range2492862/1
component: BBa_K1940000

https://synbiohub.org/public/igem/BBa_K1940001/annotation2492862/range2492862/1
type: Range
persistent identity: range2492862
display id: range2492862
version: 1
top level: BBa_K1940001
direction: direction/reverse
start: 44
end: 194
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2492864
display id: annotation2492864
version: 1
top level: BBa_K1940001
location: https://synbiohub.org/public/igem/BBa_K1940001/annotation2492864/range2492864/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1940001/annotation2492864/range2492864/1
type: Range
persistent identity: range2492864
display id: range2492864
version: 1
top level: BBa_K1940001
direction: direction/reverse
start: 203
end: 214
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2492866
display id: annotation2492866
version: 1
top level: BBa_K1940001
location: https://synbiohub.org/public/igem/BBa_K1940001/annotation2492866/range2492866/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1940001/annotation2492866/range2492866/1
type: Range
persistent identity: range2492866
display id: range2492866
version: 1
top level: BBa_K1940001
direction: direction/reverse
start: 221
end: 940
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2492873
display id: annotation2492873
version: 1
top level: BBa_K1940001
location: https://synbiohub.org/public/igem/BBa_K1940001/annotation2492873/range2492873/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1940001/annotation2492873/range2492873/1
type: Range
persistent identity: range2492873
display id: range2492873
version: 1
top level: BBa_K1940001
direction: direction/reverse
start: 949
end: 1077
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K1940000
type: ComponentDefinition
persistent identity: BBa K1940000
display id: BBa_K1940000
version: 1
was generated by: iGEM to SBOL conversion
description: anti-microRNA-34c
created: 2016-10-10T11:00:00Z
modified: 2016-10-13T09:32:59Z
mutable provenance: Homo sapiens (human),Chromosome 11, NC_000011.10 (111513439..111513515)
owned by: james
owned by: myers
mutable description: Anti- microRNA-34c can be used as a miR-34c detector. MiR-34c is significantly upregulated in Alzheimer's disease (AD) patients. Several researches have suggested that strategies to manipulate miR-34c could open a suitable novel virus-free therapeutic avenue to treat cognitive diseases. In this part, we designed 4 series antisense strand of miR-34c which are linked by 4 linkers. Anti- microRNA-34c is sensitive to the expression levels of miR-34c. And combined with a reporter, we can diagnose AD accurately and rapidly.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2407_
m user id: 30070
owner id: 30070
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: 4 copies of anti-microRNA-34c can enhance the detection sensitivity.
star: false
creator: Yujia Liu
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: anti-microRNA-34c
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K1940000_sequence/1

anti-microRNA-34c
type: SequenceAnnotation
persistent identity: annotation2492763
display id: annotation2492763
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492763/range2492763/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492763/range2492763/1
type: Range
persistent identity: range2492763
display id: range2492763
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 20
end: 42
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2492782
display id: annotation2492782
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492782/range2492782/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492782/range2492782/1
type: Range
persistent identity: range2492782
display id: range2492782
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 43
end: 49
orientation: inline

anti-microRNA-34c
type: SequenceAnnotation
persistent identity: annotation2492776
display id: annotation2492776
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492776/range2492776/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492776/range2492776/1
type: Range
persistent identity: range2492776
display id: range2492776
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 50
end: 72
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2492781
display id: annotation2492781
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492781/range2492781/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492781/range2492781/1
type: Range
persistent identity: range2492781
display id: range2492781
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 73
end: 79
orientation: inline

anti-microRNA-34c
type: SequenceAnnotation
persistent identity: annotation2492777
display id: annotation2492777
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492777/range2492777/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492777/range2492777/1
type: Range
persistent identity: range2492777
display id: range2492777
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 80
end: 102
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2492784
display id: annotation2492784
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492784/range2492784/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492784/range2492784/1
type: Range
persistent identity: range2492784
display id: range2492784
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 103
end: 109
orientation: inline

anti-microRNA-34c
type: SequenceAnnotation
persistent identity: annotation2492780
display id: annotation2492780
version: 1
top level: BBa_K1940000
location: https://synbiohub.org/public/igem/BBa_K1940000/annotation2492780/range2492780/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1940000/annotation2492780/range2492780/1
type: Range
persistent identity: range2492780
display id: range2492780
version: 1
top level: BBa_K1940000
direction: direction/forward
start: 110
end: 132
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1940001_sequence/1
type: Sequence
persistent identity: BBa K1940001 sequence
display id: BBa_K1940001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctactagagaagcttggtctcgctagaggcaatcagctaactacactgcctgggtcccgcaatcagctaactacactgcctgggtcccgcaatcagctaactacactgcctgggtcccgcaatcagctaactacactgccttactagcgagaccggtacctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1940000_sequence/1
type: Sequence
persistent identity: BBa K1940000 sequence
display id: BBa_K1940000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagcttggtctcgctagaggcaatcagctaactacactgcctgggtcccgcaatcagctaactacactgcctgggtcccgcaatcagctaactacactgcctgggtcccgcaatcagctaactacactgccttactagcgagaccggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z