LOCUS       BBa_K1940003            2399 bp    DNA     linear       26-Dec-2024
DEFINITION  lacI-placI-GFP
ACCESSION   BBa_K1940003
VERSION     BBa_K1940003.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     misc_feature    1..1308
                     /label=BBa_P0412
     CDS             1317..1404
                     /label=end of LacI coding region (inactive)
     misc_feature    1317..1516
                     /label=BBa_R0010
     misc_feature    1405..1442
                     /label=CAP binding site
     promoter        1453..1458
                     /label=-35
     promoter        1477..1482
                     /label=-10
     misc_feature    1482..1516
                     /label=LacI binding site
     misc_feature    1489..1489
                     /label=start
     promoter        1317..1516
                     /label=BBa_R0010
     misc_feature    1529..1532
                     /label=conserved
     RBS             1525..1536
                     /label=BBa_B0034
     CDS             1543..2262
                     /label=GFP protein
     CDS             1543..2262
                     /label=BBa_E0040
     stem_loop       2282..2325
                     /label=stem_loop
     misc_feature    2271..2350
                     /label=BBa_B0010
     terminator      2271..2350
                     /label=BBa_B0010
     stem_loop       2366..2385
                     /label=T7 TE
     misc_feature    2392..2392
                     /label=stop
     polyA_site      2386..2399
                     /label=polya
     misc_feature    2359..2399
                     /label=BBa_B0012
     terminator      2359..2399
                     /label=BBa_B0012
     misc_feature    1525..2399
                     /label=BBa_I13504
     misc_feature    1317..2399
                     /label=BBa_K741002
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat
     1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1501 ataacaattt cacacatact agagaaagag gagaaatact agatgcgtaa aggagaagaa
     1561 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     1621 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     1681 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
     1741 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     1801 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     1861 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     1921 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     1981 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     2041 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     2101 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     2161 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2221 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
     2281 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2341 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//