LOCUS       BBa_K1940004            1987 bp    DNA     linear       14-Nov-2024
DEFINITION  microRNA-34c detector 2.0-1-1
ACCESSION   BBa_K1940004
VERSION     BBa_K1940004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    63..85
                     /label=anti-microRNA-34c
     misc_feature    93..115
                     /label=anti-microRNA-34c
     misc_feature    123..145
                     /label=anti-microRNA-34c
     misc_feature    153..175
                     /label=anti-microRNA-34c
     misc_feature    116..122
                     /label=linker
     misc_feature    86..92
                     /label=linker
     misc_feature    146..152
                     /label=linker
     misc_feature    44..194
                     /label=BBa_K1940000
     misc_feature    207..210
                     /label=conserved
     RBS             203..214
                     /label=BBa_B0034
     CDS             224..840
                     /label=tetR
     misc_feature    841..874
                     /label=SsrA
     misc_feature    881..905
                     /label=Help:Barcodes
     CDS             221..880
                     /label=BBa_C0040
     stem_loop       925..968
                     /label=stem_loop
     misc_feature    914..993
                     /label=BBa_B0010
     terminator      914..993
                     /label=BBa_B0010
     stem_loop       1009..1028
                     /label=T7 TE
     misc_feature    1035..1035
                     /label=stop
     polyA_site      1029..1042
                     /label=polya
     misc_feature    1002..1042
                     /label=BBa_B0012
     terminator      1002..1042
                     /label=BBa_B0012
     misc_feature    203..1042
                     /label=BBa_P0440
     misc_feature    1051..1069
                     /label=TetR 1
     misc_feature    1051..1104
                     /label=BBa_R0040
     promoter        1070..1075
                     /label=-35
     misc_feature    1076..1094
                     /label=TetR 2
     promoter        1093..1098
                     /label=-10
     promoter        1051..1104
                     /label=BBa_R0040
     misc_feature    1117..1120
                     /label=conserved
     RBS             1113..1124
                     /label=BBa_B0034
     CDS             1131..1850
                     /label=GFP protein
     CDS             1131..1850
                     /label=BBa_E0040
     stem_loop       1870..1913
                     /label=stem_loop
     misc_feature    1859..1938
                     /label=BBa_B0010
     terminator      1859..1938
                     /label=BBa_B0010
     stem_loop       1954..1973
                     /label=T7 TE
     misc_feature    1980..1980
                     /label=stop
     polyA_site      1974..1987
                     /label=polya
     misc_feature    1947..1987
                     /label=BBa_B0012
     terminator      1947..1987
                     /label=BBa_B0012
     misc_feature    1113..1987
                     /label=BBa_I13504
     misc_feature    1051..1987
                     /label=BBa_K1493504
     misc_feature    203..1987
                     /label=BBa_K1940002
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaagcttg gtctcgctag
       61 aggcaatcag ctaactacac tgcctgggtc ccgcaatcag ctaactacac tgcctgggtc
      121 ccgcaatcag ctaactacac tgcctgggtc ccgcaatcag ctaactacac tgccttacta
      181 gcgagaccgg tacctactag agaaagagga gaaatactag atgtccagat tagataaaag
      241 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac
      301 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa
      361 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt
      421 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag
      481 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga
      541 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact
      601 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga
      661 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc
      721 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt
      781 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc
      841 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag
      901 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1021 ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct
     1081 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atgcgtaaag
     1141 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1201 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1261 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1321 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     1381 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     1441 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1501 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1561 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     1621 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1681 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1741 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1801 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
     1861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1921 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1981 gtttata
//