LOCUS       BBa_K1940010            2601 bp    DNA     linear       14-Nov-2024
DEFINITION  microRNA-34c detector 2.0-2-3
ACCESSION   BBa_K1940010
VERSION     BBa_K1940010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    63..85
                     /label=anti-microRNA-34c
     misc_feature    93..115
                     /label=anti-microRNA-34c
     misc_feature    123..145
                     /label=anti-microRNA-34c
     misc_feature    153..175
                     /label=anti-microRNA-34c
     misc_feature    116..122
                     /label=linker
     misc_feature    86..92
                     /label=linker
     misc_feature    146..152
                     /label=linker
     misc_feature    44..194
                     /label=BBa_K1940000
     misc_feature    207..210
                     /label=conserved
     RBS             203..214
                     /label=BBa_B0034
     misc_feature    1310..1348
                     /label=LVA
     CDS             221..1348
                     /label=lacI-LVA
     misc_feature    221..1348
                     /label=BBa_C0012
     misc_feature    1349..1373
                     /label=Help:Barcodes
     CDS             221..1348
                     /label=BBa_C0012
     stem_loop       1393..1436
                     /label=stem_loop
     misc_feature    1382..1461
                     /label=BBa_B0010
     terminator      1382..1461
                     /label=BBa_B0010
     stem_loop       1477..1496
                     /label=T7 TE
     misc_feature    1503..1503
                     /label=stop
     polyA_site      1497..1510
                     /label=polya
     misc_feature    1470..1510
                     /label=BBa_B0012
     terminator      1470..1510
                     /label=BBa_B0012
     misc_feature    203..1510
                     /label=BBa_P0412
     CDS             1519..1606
                     /label=end of LacI coding region (inactive)
     misc_feature    1519..1718
                     /label=BBa_R0010
     misc_feature    1607..1644
                     /label=CAP binding site
     promoter        1655..1660
                     /label=-35
     promoter        1679..1684
                     /label=-10
     misc_feature    1684..1718
                     /label=LacI binding site
     misc_feature    1691..1691
                     /label=start
     promoter        1519..1718
                     /label=BBa_R0010
     misc_feature    1731..1734
                     /label=conserved
     RBS             1727..1738
                     /label=BBa_B0034
     CDS             1745..2464
                     /label=GFP protein
     CDS             1745..2464
                     /label=BBa_E0040
     stem_loop       2484..2527
                     /label=stem_loop
     misc_feature    2473..2552
                     /label=BBa_B0010
     terminator      2473..2552
                     /label=BBa_B0010
     stem_loop       2568..2587
                     /label=T7 TE
     misc_feature    2594..2594
                     /label=stop
     polyA_site      2588..2601
                     /label=polya
     misc_feature    2561..2601
                     /label=BBa_B0012
     terminator      2561..2601
                     /label=BBa_B0012
     misc_feature    1727..2601
                     /label=BBa_I13504
     misc_feature    1519..2601
                     /label=BBa_K741002
     misc_feature    203..2601
                     /label=BBa_K1940003
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaagcttg gtctcgctag
       61 aggcaatcag ctaactacac tgcctgggtc ccgcaatcag ctaactacac tgcctgggtc
      121 ccgcaatcag ctaactacac tgcctgggtc ccgcaatcag ctaactacac tgccttacta
      181 gcgagaccgg tacctactag agaaagagga gaaatactag atggtgaatg tgaaaccagt
      241 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
      301 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
      361 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
      421 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
      481 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
      541 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
      601 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
      661 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
      721 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
      781 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
      841 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
      901 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
      961 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     1021 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     1081 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     1141 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     1201 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1261 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga
     1321 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga
     1381 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1441 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1501 tgcgtttata tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1561 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     1621 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     1681 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata
     1741 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1801 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1861 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1921 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1981 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     2041 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     2101 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     2161 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     2221 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     2281 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2341 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     2401 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     2461 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2521 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2581 gtgggccttt ctgcgtttat a
//