BBa_K1943006
1
BBa_K1943006
spisPink, Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
2016-08-28T11:00:00Z
2016-10-21T08:40:40Z
basic parts
Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943006 with chromoprotein spisPink(BBa_K1033932) this year and did characterization.
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
For detailed characterization data, see the experience page.
false
false
_2410_
30006
29845
9
false
no
false
Shixin Lu, Shiqiang Tang
component2512752
1
BBa_K1033932
component2512759
1
BBa_B0015
component2512750
1
BBa_K880005
annotation2512750
1
BBa_K880005
range2512750
1
1
55
annotation2512759
1
BBa_B0015
range2512759
1
748
876
annotation2512752
1
BBa_K1033932
range2512752
1
62
739
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K1033932
1
BBa_K1033932
spisPink, pink chromoprotein
2014-04-20T11:00:00Z
2015-05-08T01:08:50Z
Stylophora pistillata. The protein was first extracted and characterized by Alieva et. al. under the name spisCP (GenBank: ABB17971.1). This version is codon optimized for E coli by Genscript.
This chromoprotein from the coral Stylophora pistillata, spisPink (also known as spisCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 560 nm giving it a pink color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein spisPink has significant sequence homologies with proteins in the GFP family.
false
false
_1340_
0
9827
9
Not in stock
false
Synthesized and codon optimized for E coli by GenScript.
false
Erik Lundin
annotation2372666
1
spisPink
range2372666
1
1
678
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K880005
1
BBa_K880005
Strong promoter, strong RBS combination for high expression levels of proteins
2012-09-28T11:00:00Z
2015-05-08T01:13:40Z
It is a combination of strong promoter (J23100) and RBS (B0034).
Released HQ 2013
-J23100+B0034
-Strong promoter, strong RBS combination for high expression levels of proteins
Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
false
false
_1142_
0
9403
9
In stock
false
Enter design considerations.
false
Josh Atkinson, Mike Ferguson, and Ben Parker
component2204228
1
BBa_J23100
component2204230
1
BBa_B0034
annotation2204228
1
BBa_J23100
range2204228
1
1
35
annotation2204230
1
BBa_B0034
range2204230
1
44
55
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1943006_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtcgcactcaaaacaagcactggcagatacgatgaaaatgacgtggctgatggaaggttcggtcaacggtcacgcctttacgattgaaggcgaaggtaccggcaaaccgtatgaaggtaaacagagtggcacctttcgtgttacgaaaggcggtccgctgccgtttgcgtttgatattgtcgctccgaccctgaaatacggtttcaaatgcttcatgaaatacccggcggatattccggactactttaaactggccttcccggaaggcctgacgtacgatcgtaaaattgcgtttgaagacggcggttgtgcgaccgccacggtcgaaatgagcctgaagggtaacaccctggtgcataaaacgaactttcagggcggtaatttcccgattgatggtccggtgatgcaaaaacgcaccctgggctgggaaccgacctctgaaaaaatgacgccgtgcgatggtattatcaaaggcgacaccatcatgtacctgatggttgaaggcggtaaaacgctgaaatgtcgttatgaaaacaattaccgcgccaacaaaccagtgctgatgccgccgagccactttgtggatctgcgtctgacccgcacgaatctggataaagaaggtctggcgttcaaactggaagaatatgctgttgcccgtgtgctggaagtgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1033932_sequence
1
atgtcgcactcaaaacaagcactggcagatacgatgaaaatgacgtggctgatggaaggttcggtcaacggtcacgcctttacgattgaaggcgaaggtaccggcaaaccgtatgaaggtaaacagagtggcacctttcgtgttacgaaaggcggtccgctgccgtttgcgtttgatattgtcgctccgaccctgaaatacggtttcaaatgcttcatgaaatacccggcggatattccggactactttaaactggccttcccggaaggcctgacgtacgatcgtaaaattgcgtttgaagacggcggttgtgcgaccgccacggtcgaaatgagcctgaagggtaacaccctggtgcataaaacgaactttcagggcggtaatttcccgattgatggtccggtgatgcaaaaacgcaccctgggctgggaaccgacctctgaaaaaatgacgccgtgcgatggtattatcaaaggcgacaccatcatgtacctgatggttgaaggcggtaaaacgctgaaatgtcgttatgaaaacaattaccgcgccaacaaaccagtgctgatgccgccgagccactttgtggatctgcgtctgacccgcacgaatctggataaagaaggtctggcgttcaaactggaagaatatgctgttgcccgtgtgctggaagtgtaataa
BBa_K880005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z