BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1033932 1 BBa_K1033932 spisPink, pink chromoprotein 2014-04-20T11:00:00Z 2015-05-08T01:08:50Z Stylophora pistillata. The protein was first extracted and characterized by Alieva et. al. under the name spisCP (GenBank: ABB17971.1). This version is codon optimized for E coli by Genscript. This chromoprotein from the coral Stylophora pistillata, spisPink (also known as spisCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 560 nm giving it a pink color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein spisPink has significant sequence homologies with proteins in the GFP family. false false _1340_ 0 9827 9 Not in stock false Synthesized and codon optimized for E coli by GenScript. false Erik Lundin annotation2372666 1 spisPink range2372666 1 1 678 BBa_K1943006 1 BBa_K1943006 spisPink, Pink chromoprotein reporter system (Strong Promoter, Strong RBS) 2016-08-28T11:00:00Z 2016-10-21T08:40:40Z basic parts Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943006 with chromoprotein spisPink(BBa_K1033932) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37&#8451; overnight for LB agar plate and 37&#8451; 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page. false false _2410_ 30006 29845 9 false no false Shixin Lu, Shiqiang Tang component2512752 1 BBa_K1033932 component2512750 1 BBa_K880005 component2512759 1 BBa_B0015 annotation2512752 1 BBa_K1033932 range2512752 1 62 739 annotation2512759 1 BBa_B0015 range2512759 1 748 876 annotation2512750 1 BBa_K880005 range2512750 1 1 55 BBa_K880005 1 BBa_K880005 Strong promoter, strong RBS combination for high expression levels of proteins 2012-09-28T11:00:00Z 2015-05-08T01:13:40Z It is a combination of strong promoter (J23100) and RBS (B0034). Released HQ 2013 -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression. false false _1142_ 0 9403 9 In stock false Enter design considerations. false Josh Atkinson, Mike Ferguson, and Ben Parker component2204228 1 BBa_J23100 component2204230 1 BBa_B0034 annotation2204230 1 BBa_B0034 range2204230 1 44 55 annotation2204228 1 BBa_J23100 range2204228 1 1 35 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1943006_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtcgcactcaaaacaagcactggcagatacgatgaaaatgacgtggctgatggaaggttcggtcaacggtcacgcctttacgattgaaggcgaaggtaccggcaaaccgtatgaaggtaaacagagtggcacctttcgtgttacgaaaggcggtccgctgccgtttgcgtttgatattgtcgctccgaccctgaaatacggtttcaaatgcttcatgaaatacccggcggatattccggactactttaaactggccttcccggaaggcctgacgtacgatcgtaaaattgcgtttgaagacggcggttgtgcgaccgccacggtcgaaatgagcctgaagggtaacaccctggtgcataaaacgaactttcagggcggtaatttcccgattgatggtccggtgatgcaaaaacgcaccctgggctgggaaccgacctctgaaaaaatgacgccgtgcgatggtattatcaaaggcgacaccatcatgtacctgatggttgaaggcggtaaaacgctgaaatgtcgttatgaaaacaattaccgcgccaacaaaccagtgctgatgccgccgagccactttgtggatctgcgtctgacccgcacgaatctggataaagaaggtctggcgttcaaactggaagaatatgctgttgcccgtgtgctggaagtgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1033932_sequence 1 atgtcgcactcaaaacaagcactggcagatacgatgaaaatgacgtggctgatggaaggttcggtcaacggtcacgcctttacgattgaaggcgaaggtaccggcaaaccgtatgaaggtaaacagagtggcacctttcgtgttacgaaaggcggtccgctgccgtttgcgtttgatattgtcgctccgaccctgaaatacggtttcaaatgcttcatgaaatacccggcggatattccggactactttaaactggccttcccggaaggcctgacgtacgatcgtaaaattgcgtttgaagacggcggttgtgcgaccgccacggtcgaaatgagcctgaagggtaacaccctggtgcataaaacgaactttcagggcggtaatttcccgattgatggtccggtgatgcaaaaacgcaccctgggctgggaaccgacctctgaaaaaatgacgccgtgcgatggtattatcaaaggcgacaccatcatgtacctgatggttgaaggcggtaaaacgctgaaatgtcgttatgaaaacaattaccgcgccaacaaaccagtgctgatgccgccgagccactttgtggatctgcgtctgacccgcacgaatctggataaagaaggtctggcgttcaaactggaagaatatgctgttgcccgtgtgctggaagtgtaataa BBa_K880005_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z