BBa_K1033932
1
BBa_K1033932
spisPink, pink chromoprotein
2014-04-20T11:00:00Z
2015-05-08T01:08:50Z
Stylophora pistillata. The protein was first extracted and characterized by Alieva et. al. under the name spisCP (GenBank: ABB17971.1). This version is codon optimized for E coli by Genscript.
This chromoprotein from the coral Stylophora pistillata, spisPink (also known as spisCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 560 nm giving it a pink color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein spisPink has significant sequence homologies with proteins in the GFP family.
false
false
_1340_
0
9827
9
Not in stock
false
Synthesized and codon optimized for E coli by GenScript.
false
Erik Lundin
annotation2372666
1
spisPink
range2372666
1
1
678
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K608007
1
BBa_K608007
medium Promoter , weak RBS
2011-09-14T11:00:00Z
2015-05-08T01:12:52Z
composite
Released HQ 2013
medium Promotor , weak RBS
false
false
_780_
0
9115
9
In stock
false
composite
false
Julia M??ller
component2128663
1
BBa_J23110
component2128665
1
BBa_B0031
annotation2128665
1
BBa_B0031
range2128665
1
44
57
annotation2128663
1
BBa_J23110
range2128663
1
1
35
BBa_K1943007
1
BBa_K1943007
spisPink, Pink chromoprotein reporter system (medium Promoter , weak RBS)
2016-08-28T11:00:00Z
2016-10-21T08:40:55Z
basic parts
Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943007 with chromoprotein spisPink(BBa_K1033932) this year and did characterization.
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
For detailed characterization data, see the experience page.
false
false
_2410_
30006
29845
9
false
no
false
Shixin Lu, Shiqiang Tang
component2481935
1
BBa_K1033932
component2481933
1
BBa_K608007
component2481942
1
BBa_B0015
annotation2481935
1
BBa_K1033932
range2481935
1
64
741
annotation2481933
1
BBa_K608007
range2481933
1
1
57
annotation2481942
1
BBa_B0015
range2481942
1
750
878
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K608007_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaacc
BBa_K1943007_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaacctactagatgtcgcactcaaaacaagcactggcagatacgatgaaaatgacgtggctgatggaaggttcggtcaacggtcacgcctttacgattgaaggcgaaggtaccggcaaaccgtatgaaggtaaacagagtggcacctttcgtgttacgaaaggcggtccgctgccgtttgcgtttgatattgtcgctccgaccctgaaatacggtttcaaatgcttcatgaaatacccggcggatattccggactactttaaactggccttcccggaaggcctgacgtacgatcgtaaaattgcgtttgaagacggcggttgtgcgaccgccacggtcgaaatgagcctgaagggtaacaccctggtgcataaaacgaactttcagggcggtaatttcccgattgatggtccggtgatgcaaaaacgcaccctgggctgggaaccgacctctgaaaaaatgacgccgtgcgatggtattatcaaaggcgacaccatcatgtacctgatggttgaaggcggtaaaacgctgaaatgtcgttatgaaaacaattaccgcgccaacaaaccagtgctgatgccgccgagccactttgtggatctgcgtctgacccgcacgaatctggataaagaaggtctggcgttcaaactggaagaatatgctgttgcccgtgtgctggaagtgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_K1033932_sequence
1
atgtcgcactcaaaacaagcactggcagatacgatgaaaatgacgtggctgatggaaggttcggtcaacggtcacgcctttacgattgaaggcgaaggtaccggcaaaccgtatgaaggtaaacagagtggcacctttcgtgttacgaaaggcggtccgctgccgtttgcgtttgatattgtcgctccgaccctgaaatacggtttcaaatgcttcatgaaatacccggcggatattccggactactttaaactggccttcccggaaggcctgacgtacgatcgtaaaattgcgtttgaagacggcggttgtgcgaccgccacggtcgaaatgagcctgaagggtaacaccctggtgcataaaacgaactttcagggcggtaatttcccgattgatggtccggtgatgcaaaaacgcaccctgggctgggaaccgacctctgaaaaaatgacgccgtgcgatggtattatcaaaggcgacaccatcatgtacctgatggttgaaggcggtaaaacgctgaaatgtcgttatgaaaacaattaccgcgccaacaaaccagtgctgatgccgccgagccactttgtggatctgcgtctgacccgcacgaatctggataaagaaggtctggcgttcaaactggaagaatatgctgttgcccgtgtgctggaagtgtaataa
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z