LOCUS       BBa_K1943009             918 bp    DNA     linear       23-May-2024
DEFINITION  sfGFP+B0015, green fluorescence protein reporter system
ACCESSION   BBa_K1943009
VERSION     BBa_K1943009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=start
     CDS             62..781
                     /label=superfolder GFP coding region
     misc_feature    776..781
                     /label=stop
     CDS             62..781
                     /label=BBa_I746916
     misc_feature    1..781
                     /label=BBa_K1943008
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
     terminator      790..918
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg
      121 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg
      181 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct
      241 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca
      301 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt
      361 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt
      421 aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa
      481 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg
      541 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga
      601 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta
      661 tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct
      721 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatgatg
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttata
//