LOCUS BBa_K1945000 6439 bp DNA linear 17-Dec-2024 DEFINITION PSigA and PTic2op AND GATE ACCESSION BBa_K1945000 VERSION BBa_K1945000.1 FEATURES Location/Qualifiers misc_feature 1..725 /label=BBa_K1945008 misc_feature 734..755 /label=BBa_K1945012 CDS 764..1306 /label=BBa_K1945014 promoter 1315..1658 /label=BBa_K1945015 misc_feature 1667..1696 /label=BBa_K1945016 promoter 1705..1815 /label=BBa_K1945007 CDS 1822..3942 /label=BBa_K1945005 promoter 3951..3996 /label=BBa_I712074 RBS 4005..4017 /label=RBS-3\Weak RBS 4011..4014 /label=RBS misc_feature 4005..4017 /label=BBa_B0032 RBS 4005..4017 /label=BBa_B0032 CDS 4024..5676 /label=BBa_I712019 misc_feature 5685..5705 /label=BBa_K1945013 misc_feature 5714..6439 /label=BBa_K1945010 ORIGIN 1 ggcagtcggg cttctgggtc aggatatttg gattatgtca ccgctacgtt aggcttctcc 61 tccggcattg tggccaccct caccgccagt aaggtcaccc atcgtaaaat tcgttccatc 121 gccgcccact gcaaaaattc cctcaccgaa gcggattttc tcaataacga aattttgatc 181 catcgccaaa ccaccgctga ttggagcgcg gactatggcc aggtattgta tcgccaggat 241 ggtctaatcg aaaaggttta caccagtaat attgaacctc tccacgctga attagaacat 301 tttattcatt gtgttagggg aggtgatcaa ccctcagtgg ggggagaaca ggccctcaag 361 gccctgaagt tagccagttt aattgaagaa atggccctgg acagtcagga atggcatggg 421 ggggaagttg tgacagaata tcaagatgcc accctggccc tcagtgcgag tgtttaaatc 481 aacttaatta atgcaattat tgcgagttca aactcgataa ctttgtgaaa tattactgtt 541 gaattaatct atgactattc aatacacccc cctagccgat cgcctgttgg cctacctcgc 601 cgccgatcgc ctaaatctca gcgccaagag tagttccctc aacaccagta ttctgctcag 661 cagtgaccta ttcaatcagg aagggggaat tgtaacagcc aactatggct ttgatggtta 721 tatggtacta gaggaattcg cggccgcttc tagagtacta gagttattac ttgtagacgt 781 gccctacgcg cttgttgagt tgttcctcaa cgtttttctt gaagtgctta gcttcaaggt 841 cacggatacg accgaagcga gcctcgtcct caatggcccg accgattgcg cttgctacag 901 cctgaacggt tgtattgtca gcactggtta ggcaagccag agtggtctta atggtgatgt 961 acgctacggc ttccggcttg atttcttgca ggaactggaa ggctgtcggg cgcttgccgc 1021 gcttagcttt cacttcctca aaccagtcgt tgatgcgtgc aatcatctta gggagtaggg 1081 tagtgatgag aggcttggcg gcagcgttat ccgcaacctc accagcttta agttgacgct 1141 caaacatctt gcggaagcgt gcttcaccca tctcgtaaga ctcatgctca agggccaact 1201 gttcgcgagc taaacgctca ccgtaatggt cagccagagt gttgaacggg atagcagcca 1261 gttcgatgtc agagaagtcg ttcttagcga tgttaatcgt gttcattact agaggttttc 1321 ctcgttaact cctaagtcgt gtacaaaatt agctggtcag tcaaagttga ggccacccct 1381 aggcgttgct agatgatgtg cgctcggtca gtctattgat tcggggttct ctaacttctt 1441 cgttggggaa aaacagaatg caaggattca ggcgtaaaag tgttagagaa ggctagtcag 1501 cggtagagct tgatgaatca gtgaaaaaag acttcattga ttgtagcctc gataacaaaa 1561 aaatggggct taattgtggt ctgaccacga ctttttttca cattgctggt gagcggggac 1621 agaaagactt aaaattattg ccgatgattg acctaactta ctagagcaag cttatcgatt 1681 tcgaacccgg ggtacctact agagaattgt gagcgctcac aattgcatga gagagagctg 1741 ttgacaatta atcatcgcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 1801 cacaggaaac agaattacta gatgaaagca tttatgcaag ttgtcgaggc tgacatgctc 1861 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat 1921 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc 1981 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct 2041 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc 2101 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt 2161 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt 2221 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac 2281 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac 2341 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct 2401 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc 2461 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat 2521 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg 2581 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca 2641 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat 2701 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct 2761 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc 2821 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc 2881 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga 2941 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac 3001 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat 3061 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg 3121 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag 3181 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg 3241 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat 3301 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct 3361 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa 3421 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat 3481 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc 3541 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca 3601 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt 3661 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac 3721 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact 3781 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac 3841 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac 3901 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaat aatactagag taatacgact 3961 cactataggg aatacaagct acttgttctt tttgcatact agagtcacac aggaaagtac 4021 tagatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 4081 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 4141 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 4201 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 4261 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 4321 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 4381 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 4441 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 4501 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 4561 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 4621 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 4681 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 4741 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 4801 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 4861 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 4921 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 4981 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 5041 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 5101 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 5161 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 5221 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 5281 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 5341 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 5401 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 5461 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 5521 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 5581 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 5641 ataaaggcca agaagggcgg aaagatcgcc gtgtaatact agagtactag tagcggccgc 5701 tgcagtacta gaggatgcgg aatcccaaca gattgccttt gacaacaatg tggcctggaa 5761 taacctgggg gatttgtcca ccaccaccca acgggcctac acttcggcta ttagcacaga 5821 cacagtgcag agtgtttatg gcgttaatct ggaaaaaaac gataacattc ccattgtttt 5881 tgcgtggccc atttttccca ccacccttaa tcccacagat tttcaggtaa tgcttaacac 5941 gggggaaatt gtcaccccgg tgatcgcctc tttgattccc aacagtgaat acaacgaacg 6001 gcaaacggta gtaattacgg gcaattttgg taatcgttta accccaggca cggagggagc 6061 gatttatccc gtttccgtag gcacagtgtt ggacagtact cctttggaaa tggtgggacc 6121 caacggcccg gtcagtgcgg tgggtattac cattgatagt ctcaacccct acgtggccgg 6181 caatggtccc aaaattgtcg ccgctaagtt agaccgcttc agtgacctgg gggaaggggc 6241 tcccctctgg ttagccacca atcaaaataa cagtggcggg gatttatatg gagaccaagc 6301 ccaatttcgt ttgcgaattt acaccagcgc cggtttttcc cccgatggca ttgccagttt 6361 actacccaca gaatttgaac ggtattttca actccaagcg gaagatatta cgggacggac 6421 agttatccta acccaaact //