C-terminal fragment of T7 RNA Polymerase
This is the iGEM BioBrick Prefix sequence.
Reverse complement of N-terminal fragment of split T7 RNA polymerase
This polymerase is from bacteriophage T7.
This is the reverse complement of the part described here: http://parts.igem.org/Part:BBa_K1945004.
Reverse complement of SigA promoter and RBS
As we amplified this part from Synechocystis sp. PCC 6803, all the native sequences between the promoter and the RBS are included.
This is the native SigA promoter and RBS sequences amplified from Synechocystis sp. PCC 6803.
DNA sequence in http://parts.igem.org/Part:BBa_K1945001.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Firefly luciferase - luciferase from Photinus pyralis
This firefly luciferase comes from Promega PGL3 vector.
Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
Part is cloned into pSBAK3 vector with terminator.
T7 promoter (strong promoter from T7 bacteriophage)
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
This is the iGEM BioBrick Suffix sequence.
Conversion of the iGEM parts registry to SBOL2.1