BBa_K1946002
1
BBa_K1946002
sgRNA targeting LacI
2016-10-12T11:00:00Z
2016-10-14T06:17:48Z
This part comes from the paper Liu, Yuchen, Yayue Zeng, Li Liu, Chengle Zhuang, Xing Fu, Weiren Huang, and Zhiming Cai. "Synthesizing AND Gate Genetic Circuits Based on CRISPR-Cas9 for Identification of Bladder Cancer Cells." Nature Communications Nat Comms 5 (2014): 5393.
This part has a sgRNA targeting lacI. It also has 5' and 3' ribozyme which cleave the RNA molecule with self catalysis so that any sequence can be added to 5' or 3' to this part without preventing Cas9 binding and its activity.
false
false
_2413_
29843
29843
9
false
.
false
Musa Efe Işılak
annotation2497324
1
LacI targeting region
range2497324
1
59
78
annotation2497327
1
Cas9 recognition site
range2497327
1
79
162
annotation2497285
1
5
range2497285
1
1
58
annotation2497329
1
3' ribozyme
range2497329
1
163
205
BBa_K1946002_sequence
1
gtcgacacgtggcctgatgagtccgtgaggacgaaacggtaggaattcctaccgtcgccacgtttctgcgaaaacggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttaccggagtcgactccggtctgatgagtccgtgaggacgaaaaaaaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z