LOCUS       BBa_K1946004            3087 bp    DNA     linear       25-Nov-2024
DEFINITION  Toluene/Xylene Sensor
ACCESSION   BBa_K1946004
VERSION     BBa_K1946004.1  
FEATURES             Location/Qualifiers
     promoter        3..57
                     /label=pL(tetO)
     RBS             73..77
                     /label=RBS
     misc_feature    87..1784
                     /label=XylR (K1946005) 
     misc_feature    1788..1892
                     /label=rrnB T1 (B0010) 
     misc_feature    1899..2220
                     /label=pu (K1946005)
     RBS             2227..2241
                     /label=RBS
     misc_feature    2254..2967
                     /label=sfGFP (I746916)
     misc_feature    2977..3081
                     /label=rrnB T1 (B0010)
ORIGIN
        1 ggtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcacagag
       61 cacatttcac acaggaaaca gtattcatgt ctctgacata caaaccaaag atgcagcacg
      121 aagacatgca ggatcttagc tctcagattc gtttcgttgc cgccgagggg aaaatttggt
      181 tgggcgaaca acgtatgctg gtcatgcaat tgagcacact ggcctcgttt cgtcgcgaga
      241 tcattagtct gattggcgtc gaacgcgcca agggattttt cctgcgcctt ggctatcagt
      301 ctggacttat ggacgctgag ttagcgcgca aactgcgtcc agctatgcgt gaggaagagg
      361 tgttcttagc aggcccccaa ttatacgcct taaaaggaat ggtgaaagtc cgcttgctga
      421 ctatggatat tgctatccgc gacggacgct ttaacgtaga ggcggagtgg attgatagtt
      481 ttgaagtcga catctgccgc actgagcttg gattgatgaa cgaacccgtc tgctggactg
      541 tactgggata cgcatcgggt tacggcagcg cttttatggg ccgtcgcatc attttccaag
      601 aaacgagctg tcgcgggtgt ggggacgata aatgcttgat cgtcggcaag acagccgaag
      661 agtggggtga tgtaagctct tttgaagctt attttaagtc cgatcctatt gtcgatgagc
      721 gctacgaact tcaaactcaa gtagcaaatc tgcgtaatcg cctgaagcag tacgacggtc
      781 agtactacgg gattgggcat agtccagcgt ataaacgcat ttgtgaaacg atcgacaaag
      841 cggctcgtgg tcgtgtgagt gtcctgttat taggtgagac aggagtaggg aaagaggtaa
      901 ttgcccgctc agtccacttg cgcagcgagc gtgccgagca accttttgta gccgtgaatt
      961 gcgctgcaat tccacccgac ttgatcgaaa gtgagctttt cggggtcgac aaaggagcat
     1021 acaccggtgc agtaaatgcc cgtgccggtc gctttgaacg tgccaacggg ggcacaatct
     1081 ttcttgacga agtaatcgag cttacaccac gtgcacaagc caccttatta cgtgttttgc
     1141 aggagggcga actggaacgt gtaggaggag accgcacccg taaagtagat gttcgtttaa
     1201 ttactgcgac taatgaaaac ttggaggagg ctgtaaagat gggacgcttc cgcgcggacc
     1261 ttttctttcg ccttaacgtt tttccggttc atattcctcc cttacgtgag cgcgtcgagg
     1321 acattccgtt gttggtagaa catttccttc gtcgccatca caaggagtat gggaagaaaa
     1381 cactgggatt atccgaccgt gctatggagg catgcttgca ttaccaatgg cccggaaata
     1441 tccgcgaact ggaaaatgcg ttagaacgtg gagtgatctt aacagagtca aatgagtcca
     1501 ttaacgtgga atcattgttt ccagggcttg ctacggcgac agaaggagac cgtctgagca
     1561 gtgaaggacg cttagaggag gaatctggcg atagctggtt ccgtcaaatc attgaccaag
     1621 gtgttagttt ggaggatctt gaggctggcc tgatgcgtac tgccatggac cgctgtggac
     1681 aaaacatctc tcaagctgcg cgtttacttg gattaacacg cccagcgatg gcgtatcgtc
     1741 tgaagaagtt ggatcctagt ttgtcggtga aggctatggg ccgttaaggc atcaaataaa
     1801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1861 tctcctgagt aggacaaatc cgccgcccta gagacgtcgc ccgggaaagc gcgatgaacc
     1921 ttttttatcg ctgccttgat caaatcgaca ggtggttatg cgcgattgat gatttgctca
     1981 aatacagcca gcgtgctgta gattttctct catacccccc ctttcttttt tacaaagaaa
     2041 atcaataatt tagatgaaat aaggggatcg gtataagcaa tggcatggcg gttgctagct
     2101 atacgagact taaaataaaa atagtggtga cccttcaatg ttgtattttc tcaactctgt
     2161 tcagattggt tgctttcgcc atgtatatcc tcaaagcggg ccagccgtag ccgttacgct
     2221 cctaggatta aagaggagaa agagctcaga tctatgcgta aaggcgaaga gctgttcact
     2281 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
     2341 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact
     2401 actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag
     2461 tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg
     2521 gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt
     2581 gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac
     2641 tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat
     2701 gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac
     2761 aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt
     2821 gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaaa
     2881 gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc
     2941 acgcatggta tggatgaact gtacaaactt aagtaaggca tcaaataaaa cgaaaggctc
     3001 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     3061 ggacaaatcc gccgccctag acccggg
//