BBa_K1946004 1 BBa_K1946004 Toluene/Xylene Sensor 2016-10-18T11:00:00Z 2016-10-19T10:23:29Z The source of XylR (BBa_K1946005) can be found at GenBank: AAB59162.1 . pu promoter (BBa_K1946006) was synthetically synthesized from GeneWiz based on Pseudomonas putida TOL plasmid. sfGFP sequence can be accessed in the registry at BBa_I746916. This part responds to the presence of toluene, m-, p- or o-xylenes and produces a green fluorescent signal. The transcriptional regulatory protein XylR recognizes these organic molecules and activates pu promoter which in turn will lead to the synthesis of sfGFP. false false _2413_ 29837 29837 9 Not in stock false XylR (BBa_K1946005) in this composite part was codon optimized by us for E.coli K12. false Ayşe Naz Ozant??rk annotation2521523 1 pL(tetO) range2521523 1 3 57 annotation2521528 1 RBS range2521528 1 2227 2241 annotation2521527 1 pu (K1946005) range2521527 1 1899 2220 annotation2521530 1 rrnB T1 (B0010) range2521530 1 2977 3081 annotation2521529 1 sfGFP (I746916) range2521529 1 2254 2967 annotation2521526 1 rrnB T1 (B0010) range2521526 1 1788 1892 annotation2521524 1 RBS range2521524 1 73 77 annotation2521525 1 XylR (K1946005) range2521525 1 87 1784 BBa_K1946004_sequence 1 ggtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacagagcacatttcacacaggaaacagtattcatgtctctgacatacaaaccaaagatgcagcacgaagacatgcaggatcttagctctcagattcgtttcgttgccgccgaggggaaaatttggttgggcgaacaacgtatgctggtcatgcaattgagcacactggcctcgtttcgtcgcgagatcattagtctgattggcgtcgaacgcgccaagggatttttcctgcgccttggctatcagtctggacttatggacgctgagttagcgcgcaaactgcgtccagctatgcgtgaggaagaggtgttcttagcaggcccccaattatacgccttaaaaggaatggtgaaagtccgcttgctgactatggatattgctatccgcgacggacgctttaacgtagaggcggagtggattgatagttttgaagtcgacatctgccgcactgagcttggattgatgaacgaacccgtctgctggactgtactgggatacgcatcgggttacggcagcgcttttatgggccgtcgcatcattttccaagaaacgagctgtcgcgggtgtggggacgataaatgcttgatcgtcggcaagacagccgaagagtggggtgatgtaagctcttttgaagcttattttaagtccgatcctattgtcgatgagcgctacgaacttcaaactcaagtagcaaatctgcgtaatcgcctgaagcagtacgacggtcagtactacgggattgggcatagtccagcgtataaacgcatttgtgaaacgatcgacaaagcggctcgtggtcgtgtgagtgtcctgttattaggtgagacaggagtagggaaagaggtaattgcccgctcagtccacttgcgcagcgagcgtgccgagcaaccttttgtagccgtgaattgcgctgcaattccacccgacttgatcgaaagtgagcttttcggggtcgacaaaggagcatacaccggtgcagtaaatgcccgtgccggtcgctttgaacgtgccaacgggggcacaatctttcttgacgaagtaatcgagcttacaccacgtgcacaagccaccttattacgtgttttgcaggagggcgaactggaacgtgtaggaggagaccgcacccgtaaagtagatgttcgtttaattactgcgactaatgaaaacttggaggaggctgtaaagatgggacgcttccgcgcggaccttttctttcgccttaacgtttttccggttcatattcctcccttacgtgagcgcgtcgaggacattccgttgttggtagaacatttccttcgtcgccatcacaaggagtatgggaagaaaacactgggattatccgaccgtgctatggaggcatgcttgcattaccaatggcccggaaatatccgcgaactggaaaatgcgttagaacgtggagtgatcttaacagagtcaaatgagtccattaacgtggaatcattgtttccagggcttgctacggcgacagaaggagaccgtctgagcagtgaaggacgcttagaggaggaatctggcgatagctggttccgtcaaatcattgaccaaggtgttagtttggaggatcttgaggctggcctgatgcgtactgccatggaccgctgtggacaaaacatctctcaagctgcgcgtttacttggattaacacgcccagcgatggcgtatcgtctgaagaagttggatcctagtttgtcggtgaaggctatgggccgttaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagagacgtcgcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctcctaggattaaagaggagaaagagctcagatctatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaacttaagtaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacccggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z