BBa_K1947000
1
Mms13
Mms13
2016-10-12T11:00:00Z
2016-10-14T02:57:17Z
This gene comes from the genome of Magnetospirillum magneticum AMB-1.
This part is the coding sequence of Mms13. Mms13 is a particle protein, which was found on the membrane of Magnetosome in Magnetospirillum magneticum AMB-1. Our purpose is to display a protein (in our case it is Spycatcher) on the membrane of Magnetosome by fusing the protein with Mms13.
false
false
_2414_
33528
34115
9
false
This part is used to display a recombinant protein on the membrane of Magnetosome.
false
zhang xucheng
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1486004
1
BBa_K1486004
flexible linker 2x (GGGGS)
2014-08-11T11:00:00Z
2015-05-08T01:10:42Z
Synthetic.
Flexible linker used to fuse two proteins.
false
false
_1866_
0
21042
9
Not in stock
false
Designed considering the flexibility and separation needed between our fused proteins.
false
iGem EPFL 2014
BBa_K1947018
1
BBa_K1947018
We verify the expression effect of Mms13.
2016-10-13T11:00:00Z
2016-10-15T05:00:16Z
Magnetic bacterium Magnetospirillum magneticum AMB-1 that can produce magnet particles, also called Magnetosome, covered by a bilayer phospholipid membrane, in which Mms13 protein is anchored.
Long description: In this part, Mms13 and EGFP cDNAs are fused together to produce a fusion protein. Then it can prove whether Mms13 and EGFP can be successfully expressed in AMB-1 by the EGFP florescence.
true
false
_2414_
33797
33797
9
false
EGFP was derived from the laboratory and used for intracellular expression as a reporter.
false
Jie Tang
component2501628
1
BBa_K1947000
component2501629
1
BBa_K1486004
component2501631
1
BBa_E0040
annotation2501629
1
BBa_K1486004
range2501629
1
387
416
annotation2501631
1
BBa_E0040
range2501631
1
423
1142
annotation2501628
1
BBa_K1947000
range2501628
1
1
378
BBa_K1947000_sequence
1
atgccctttcaccttgccccctatctggcgaaatccgttcccggcgtcggcgttctcggcgccctggtcggcggcgccgccgccttggccaagaacgtccgcctcctgaaggaaaagcgcatcaccaataccgaagcggccatcgataccggcaaggaaaccgtcggcgccggcctggccaccgcgctttccgccgtggccgcgaccgccgtcggcggcggcctggttgtatcgctgggcaccgccttggtggccggcgttgccgccaaatatgcctgggatcgcggcgtcgatctggtcgagaaggaactgaaccgcggcaaagctgccaacggcgcttccgacgaggacatcttgcgggacgaactggcctaataa
BBa_K1947018_sequence
1
atgccctttcaccttgccccctatctggcgaaatccgttcccggcgtcggcgttctcggcgccctggtcggcggcgccgccgccttggccaagaacgtccgcctcctgaaggaaaagcgcatcaccaataccgaagcggccatcgataccggcaaggaaaccgtcggcgccggcctggccaccgcgctttccgccgtggccgcgaccgccgtcggcggcggcctggttgtatcgctgggcaccgccttggtggccggcgttgccgccaaatatgcctgggatcgcggcgtcgatctggtcgagaaggaactgaaccgcggcaaagctgccaacggcgcttccgacgaggacatcttgcgggacgaactggcctaataatactagagggtggtggtggttctggtggtggtggttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1486004_sequence
1
ggtggtggtggttctggtggtggtggttct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z