BBa_K1947020 1 BBa_K1947020 This polypeptide will be fused with the recombinant protein which we want to purify. 2016-10-13T11:00:00Z 2016-10-14T01:54:38Z Streptococcus pyogenes fibro-nectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys31 and Asp117. This domain can be splitted into two fragments: the small one is named as Spytag, while the big one is Spycatcher. Spytag is separated from the second immunoglobulin-like collagen adhesin domain (CnaB2) which is come from the fibronectin binding protein FbaB. There is a highly specific recognition and covalent conjugation between Spytag (a peptide with 13 amino acids) and Spycatcher (a small protein consisting of 138 residues). A protein of interest with a Spytag at its N- or C-terminus is expressed in E. coli and present in bacterial lysate.This part encodes a polypeptide that can combined with Spycatcher, through a covalent bond. In our case this polypeptide will be fused with the recombinant protein which we want to purify. true false _2414_ 33797 33797 9 false This part will be fused with the recombinant protein which we want to purify. false Jie Tang BBa_K1947020_sequence 1 gctcatattgtcatggttgatgcttacaagccaactaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z