LOCUS BBa_K1947024 1401 bp DNA linear 22-Dec-2024 DEFINITION Improve the protein purification. ACCESSION BBa_K1947024 VERSION BBa_K1947024.1 FEATURES Location/Qualifiers CDS 1..666 /label=amilCP CDS 1..669 /label=BBa_K592009 misc_feature 678..707 /label=BBa_K1486004 misc_feature 714..1316 /label=BBa_K1947002 misc_feature 1325..1354 /label=BBa_K1486004 misc_feature 1363..1401 /label=SpyTag misc_feature 1381..1383 /label=Reactive Asp for isopeptide forming reaction CDS 1363..1401 /label=BBa_K1159201 ORIGIN 1 atgagtgtga tcgctaaaca aatgacctac aaggtttata tgtcaggcac ggtcaatgga 61 cactactttg aggtcgaagg cgatggaaaa ggtaagccct acgaggggga gcagacggta 121 aagctcactg tcaccaaggg cggacctctg ccatttgctt gggatatttt atcaccacag 181 tgtcagtacg gaagcatacc attcaccaag taccctgaag acatccctga ctatgtaaag 241 cagtcattcc cggagggcta tacatgggag aggatcatga actttgaaga tggtgcagtg 301 tgtactgtca gcaatgattc cagcatccaa ggcaactgtt tcatctacca tgtcaagttc 361 tctggtttga actttcctcc caatggacct gtcatgcaga agaagacaca gggctgggaa 421 cccaacactg agcgtctctt tgcacgagat ggaatgctgc taggaaacaa ctttatggct 481 ctgaagttag aaggaggcgg tcactatttg tgtgaattta aaactactta caaggcaaag 541 aagcctgtga agatgccagg gtatcactat gttgaccgca aactggatgt aaccaatcac 601 aacaaggatt acacttcggt tgagcagtgt gaaatttcca ttgcacgcaa acctgtggtc 661 gcctaataat actagagggt ggtggtggtt ctggtggtgg tggttcttac tagatgtgca 721 tcacgggaga tgcgctggtt gccctacccg agggcgagtc ggtacgcatc gccgacatca 781 tcccgtgctc gccgaccggc tgttccactc cggcgagcat ccggtgtaca cggtgcgtac 841 ggtcggtgcc gggtgcgcgg cccaacagtg acaacgccat cgacctgaaa gtccttgacc 901 ggcatggcaa aggtctgcgt gtgacgggca ccgcgaacca cccgttgttg tgtttggtcg 961 acgtcgccgg ggtgccgacc ctgctgtgga agctgatcga cgaaatcaag ccgggcgatt 1021 acgcggtgat tcaacgcagc gcattcagcg tcgactgtgc aggttttgcc cgcggaaaac 1081 ccgaatttgc gcccacaacc tacacagtcg gcgtccctgg actggtgcgt ttcttggaag 1141 cacaccaccg agacccggac gcccaagcta tcgccgacga gctgaccgac gggcggttct 1201 actacgcgaa agtcgccagt gtcaccgacg ccggcgtgca gccggtgtat agccttcgtg 1261 tcgacacggc agaccacgcg tttatcacca acgggttcgt cagccacgcg taataatact 1321 agagggtggt ggtggttctg gtggtggtgg ttcttactag aggctcatat tgtcatggtt 1381 gatgcttaca agccaactaa g //