BBa_K1947029
1
BBa_K1947029
This part is used to improve the protein purification.
2016-10-13T11:00:00Z
2016-10-15T05:40:52Z
Protein splicing elements (termed inteins) were described as in-frame insertions in the Saccharomyces cerevisiae VMA1 gene.
TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn???t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease.
false
false
_2414_
33797
33795
9
true
GS linker is used to construct the fusion protein. Amilcp does not have a termination codon.
false
Xuechun Wang
component2505148
1
BBa_K1486004
component2505153
1
BBa_K1159201
component2505150
1
BBa_K1486004
component2505149
1
BBa_K1947002
component2505147
1
BBa_K592009
annotation2505147
1
BBa_K592009
range2505147
1
1
669
annotation2505150
1
BBa_K1486004
range2505150
1
1325
1354
annotation2505148
1
BBa_K1486004
range2505148
1
678
707
annotation2505149
1
BBa_K1947002
range2505149
1
714
1316
annotation2505153
1
BBa_K1159201
range2505153
1
1363
1401
BBa_K1159201
1
BBa_K1159201
Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]
2013-09-13T11:00:00Z
2015-05-08T01:09:31Z
''Streptococcus pyogenes''
This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
annotation2343481
1
SpyTag
range2343481
1
1
39
annotation2343512
1
Reactive Asp for isopeptide forming reaction
range2343512
1
19
21
BBa_K1947002
1
Intein
Intein
2016-10-12T11:00:00Z
2016-10-14T02:59:19Z
This sequence comes form the gyrA gene of Mycobacterium xenopi
This sequence encodes a short polypeptide which can be specifically recognized and spliced by DL-Dithiothreitol(DTT). In our case we add this short sequence between the recombinant protein which we want to purify and the linker protein Spytag, in order to separate the recombinant protein form the Magnetosome.
false
false
_2414_
33528
34115
9
false
Our project is to use Magnetosome to purify recombinant protein, and we need to remove the protein from the compound after the protein we are interested combined with Magnetosome. As this short polypeptide can be spliced by DTT, we can meet this need by adding the intein between the recombinant protein and magnetosome.
false
zhang xucheng
BBa_K592009
1
amilCP
amilCP, blue chromoprotein
2011-09-17T11:00:00Z
2015-05-08T01:12:48Z
Acropora millepora
Released HQ 2013
This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
false
false
_763_
0
7929
9
In stock
true
Illegal internal restriction site had to be removed (EcoRI).
false
Lei Sun
annotation2131628
1
amilCP
range2131628
1
1
666
BBa_K1486004
1
BBa_K1486004
flexible linker 2x (GGGGS)
2014-08-11T11:00:00Z
2015-05-08T01:10:42Z
Synthetic.
Flexible linker used to fuse two proteins.
false
false
_1866_
0
21042
9
Not in stock
false
Designed considering the flexibility and separation needed between our fused proteins.
false
iGem EPFL 2014
BBa_K1947029_sequence
1
atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataatactagagggtggtggtggttctggtggtggtggttcttactagatgtgcatcacgggagatgcgctggttgccctacccgagggcgagtcggtacgcatcgccgacatcatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcggtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcggaaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgagacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcaccaacgggttcgtcagccacgcgtaataatactagagggtggtggtggttctggtggtggtggttcttactagaggctcatattgtcatggttgatgcttacaagccaactaag
BBa_K592009_sequence
1
atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
BBa_K1947002_sequence
1
atgtgcatcacgggagatgcgctggttgccctacccgagggcgagtcggtacgcatcgccgacatcatcccgtgctcgccgaccggctgttccactccggcgagcatccggtgtacacggtgcgtacggtcggtgccgggtgcgcggcccaacagtgacaacgccatcgacctgaaagtccttgaccggcatggcaaaggtctgcgtgtgacgggcaccgcgaaccacccgttgttgtgtttggtcgacgtcgccggggtgccgaccctgctgtggaagctgatcgacgaaatcaagccgggcgattacgcggtgattcaacgcagcgcattcagcgtcgactgtgcaggttttgcccgcggaaaacccgaatttgcgcccacaacctacacagtcggcgtccctggactggtgcgtttcttggaagcacaccaccgagacccggacgcccaagctatcgccgacgagctgaccgacgggcggttctactacgcgaaagtcgccagtgtcaccgacgccggcgtgcagccggtgtatagccttcgtgtcgacacggcagaccacgcgtttatcaccaacgggttcgtcagccacgcgtaataa
BBa_K1159201_sequence
1
gctcatattgtcatggttgatgcttacaagccaactaag
BBa_K1486004_sequence
1
ggtggtggtggttctggtggtggtggttct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z