LOCUS       BBa_K1949022             523 bp    DNA     linear       11-May-2025
DEFINITION  Plac-rbs-yafN
ACCESSION   BBa_K1949022
VERSION     BBa_K1949022.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    235..235
                     /label=mutation A to C
     CDS             227..523
                     /label=BBa_K1949020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcgcattct
      241 cgctgaaaaa tcggtcaata tcactgagtt acgtaaaaac ccagctaaat actttattga
      301 tcaaccggtt gcggttcttt ctaataatcg ccccgcagga tatctcttaa gtgccagcgc
      361 attcgaagcg ttaatggaca tgcttgctga acaagaggag aaaaagccca taaaggcgcg
      421 cttccgtcca agtgctgcaa gattagagga aattacacgc cgcgctgaac aatatcttaa
      481 tgatatgacg gatgatgatt tcaatgactt taaggaataa taa
//