BBa_K1949030
1
BBa_K1949030
yafO
2016-10-05T11:00:00Z
2016-10-08T07:23:09Z
This part is derived from E.coli,BM26, amplified by using PCR.
toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E.coli genome. They can inhibit cell growth or induce cell killing. yafO is one of the toxin and is co-expressed with yafN.
　
false
false
_2416_
32067
31979
9
false
sequence confirmed.
false
Yoshio Takata
BBa_K1949031
1
BBa_K1949031
rbs-yafO
2016-10-05T11:00:00Z
2016-10-14T09:15:07Z
This part is derived from E.coli,BM26, amplified by using PCR.
This part consists of RBS(<partinfo>BBa_B0034</partinfo>) + yafO(<partinfo>BBa_K1949030</partinfo>)
Toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E.coli genome. They can inhibit cell growth or induce cell killing. yafO is one of the toxin and is co-expressed with yafN.
　
false
false
_2416_
31979
31979
9
false
sequence confirmed.
false
Yoshio Takata
component2487954
1
BBa_K1949030
component2487953
1
BBa_B0034
annotation2487953
1
BBa_B0034
range2487953
1
1
12
annotation2487954
1
BBa_K1949030
range2487954
1
19
420
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1949031_sequence
1
aaagaggagaaatactagatgcgggtattcaaaacaaaacttattcgcctgcaacttacagcagaggaacttgatgcgttaacggcggattttatttcctataagcgtgacggtgttttgccagatatatttggtcgcgatgcactctacgacgactcctttacctggccattaatcaaatttgagcgagttgctcatattcatctggcaaatgagaataatccatttccgccacagttgcgccaattcagcagaacgaatgacgaagcgcatttggtatattgtcagggggcgtttgatgagcaagcatggttgctcattgccattctgaaacctgaacctcataaactggctcgagataacaaccaaatgcataaaattgggaaaatggcagaagcgtttcgcatgcgtttttaataa
BBa_K1949030_sequence
1
atgcgggtattcaaaacaaaacttattcgcctgcaacttacagcagaggaacttgatgcgttaacggcggattttatttcctataagcgtgacggtgttttgccagatatatttggtcgcgatgcactctacgacgactcctttacctggccattaatcaaatttgagcgagttgctcatattcatctggcaaatgagaataatccatttccgccacagttgcgccaattcagcagaacgaatgacgaagcgcatttggtatattgtcagggggcgtttgatgagcaagcatggttgctcattgccattctgaaacctgaacctcataaactggctcgagataacaaccaaatgcataaaattgggaaaatggcagaagcgtttcgcatgcgtttttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z