BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1949051 1 BBa_K1949051 rbs-amiE 2016-10-05T11:00:00Z 2016-10-14T09:14:13Z This gene was isolated from Acinetobacter sp. strain Ooi24. We artificially synthesized this. This gene codes for protein AmiE. AmiE is an acylase that is capable of degrading long chain N-acyl homoserine lactone (AHL) molecules with acyl chains longer than six carbons. false false _2416_ 31979 31979 9 false We applied a site-specific mutation to the amiE in order to destroy a recognition site of EcoRI. We changed the 291th base A to G. false Yoshio Takata component2487200 1 BBa_K1949050 component2487199 1 BBa_B0034 annotation2487200 1 BBa_K1949050 range2487200 1 19 1494 annotation2487199 1 BBa_B0034 range2487199 1 1 12 BBa_K1949050 1 BBa_K1949050 amiE 2016-10-05T11:00:00Z 2016-10-08T07:25:13Z This gene was isolated from Acinetobacter sp. strain Ooi24. fwd: rev: This gene codes for protein AmiE. AmiE is an acylase that is capable of degrading long chain N-acyl homoserine lactone (AHL) molecules with acyl chains longer than six carbons. false false _2416_ 32067 31979 9 false sequence confirmed false Yoshio Takata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1949051_sequence 1 aaagaggagaaatactagatgccaaaagattttgtttatgatgatgcaactgcgttagtacaatccatccaaaatggacaaatcagtagccgtgaattgctggaatcacagattgaacggattaataaatacaacaaacacatcaatagttgtgttgcatttgattatgacaatgcaaggattttagcagatcaggctgacctcgccatcaagcgacaagccccacttggtaaattacatggcattccgatgaccatcaaagatgcctttaaagtgaaaggcatgccgtgtaccgatggcaatccagagttccaacactatgtccccaccgaaaatgctgtttcggtcaataaattggtcggtgctggagccattccttttgcaaaaaccaatgtaccctttaaatgtgcagatattcaaacctacaacaaattttatggtactgccaataatccatggaatttaaatctcacaacaggcggctcatcaggtggttctgctgcggcactagcatcgggatttacaccgattgagcttggtagtgatattggtggttctattcgaacaccagcacatttttgtggtgtctatggtcataaatccacctatggattgattgatttcagaggacacatcatgaattacgaggacgagttatcccaacctgaccttgccgtcattggtccaatggctcgttcagcacgggacttaagtttattattagatgttttagtcgagccaaatgcggatgactttagagcttttaaactcaaggaatctgaaaaacaaaccattcaagagtttaaagtattattctggatggatgatgaatcgtgtccaatcgattcacggctaaagaaaaaatacgatcaacttttgcaaaccctgcaaactgccaaggttaacgtggatgtaggtcgtccaaaaaattgggattttgatgaaatatttaaatcctacgccactcgattgatcagccaaatgacttttgcagatcctaaactaagtcgattgtccatgtcggcatctacgccattaatcaaattacttaatggtaaagcaggcattccgcctttggcctatggatttacacaaggtgctaacctgagtcatgcagaatggcttgccaaatatgaagagtcattgcatatcaagcaaaaatgtcttgaaatttttgcagaatacgacgtcattatttgcccacctattttgactctagcgtttgaacacaatcataaagaacctttgatgtttcgcacgctacaagtcgatggaagaaaacgtttctatcttgagttattcaaatggatttcgccagcaactgtatttgggttgccatcaacctctgcaccgataggcttatctgaggacaatctaccagtcaatattcaaatattgggacaaccgtatgcagacaaagtcaccattaaatttgctgaactgcttgcacaaattacggatggattccaaaaaccgcctttggatttttaataa BBa_K1949050_sequence 1 atgccaaaagattttgtttatgatgatgcaactgcgttagtacaatccatccaaaatggacaaatcagtagccgtgaattgctggaatcacagattgaacggattaataaatacaacaaacacatcaatagttgtgttgcatttgattatgacaatgcaaggattttagcagatcaggctgacctcgccatcaagcgacaagccccacttggtaaattacatggcattccgatgaccatcaaagatgcctttaaagtgaaaggcatgccgtgtaccgatggcaatccagagttccaacactatgtccccaccgaaaatgctgtttcggtcaataaattggtcggtgctggagccattccttttgcaaaaaccaatgtaccctttaaatgtgcagatattcaaacctacaacaaattttatggtactgccaataatccatggaatttaaatctcacaacaggcggctcatcaggtggttctgctgcggcactagcatcgggatttacaccgattgagcttggtagtgatattggtggttctattcgaacaccagcacatttttgtggtgtctatggtcataaatccacctatggattgattgatttcagaggacacatcatgaattacgaggacgagttatcccaacctgaccttgccgtcattggtccaatggctcgttcagcacgggacttaagtttattattagatgttttagtcgagccaaatgcggatgactttagagcttttaaactcaaggaatctgaaaaacaaaccattcaagagtttaaagtattattctggatggatgatgaatcgtgtccaatcgattcacggctaaagaaaaaatacgatcaacttttgcaaaccctgcaaactgccaaggttaacgtggatgtaggtcgtccaaaaaattgggattttgatgaaatatttaaatcctacgccactcgattgatcagccaaatgacttttgcagatcctaaactaagtcgattgtccatgtcggcatctacgccattaatcaaattacttaatggtaaagcaggcattccgcctttggcctatggatttacacaaggtgctaacctgagtcatgcagaatggcttgccaaatatgaagagtcattgcatatcaagcaaaaatgtcttgaaatttttgcagaatacgacgtcattatttgcccacctattttgactctagcgtttgaacacaatcataaagaacctttgatgtttcgcacgctacaagtcgatggaagaaaacgtttctatcttgagttattcaaatggatttcgccagcaactgtatttgggttgccatcaacctctgcaccgataggcttatctgaggacaatctaccagtcaatattcaaatattgggacaaccgtatgcagacaaagtcaccattaaatttgctgaactgcttgcacaaattacggatggattccaaaaaccgcctttggatttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z