LOCUS       BBa_K1949052            2712 bp    DNA     linear       28-Jun-2024
DEFINITION  PBAD-rbs-amiE
ACCESSION   BBa_K1949052
VERSION     BBa_K1949052.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..2712
                     /label=BBa_K1949050
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc caaaagattt tgtttatgat
     1261 gatgcaactg cgttagtaca atccatccaa aatggacaaa tcagtagccg tgaattgctg
     1321 gaatcacaga ttgaacggat taataaatac aacaaacaca tcaatagttg tgttgcattt
     1381 gattatgaca atgcaaggat tttagcagat caggctgacc tcgccatcaa gcgacaagcc
     1441 ccacttggta aattacatgg cattccgatg accatcaaag atgcctttaa agtgaaaggc
     1501 atgccgtgta ccgatggcaa tccagagttc caacactatg tccccaccga aaatgctgtt
     1561 tcggtcaata aattggtcgg tgctggagcc attccttttg caaaaaccaa tgtacccttt
     1621 aaatgtgcag atattcaaac ctacaacaaa ttttatggta ctgccaataa tccatggaat
     1681 ttaaatctca caacaggcgg ctcatcaggt ggttctgctg cggcactagc atcgggattt
     1741 acaccgattg agcttggtag tgatattggt ggttctattc gaacaccagc acatttttgt
     1801 ggtgtctatg gtcataaatc cacctatgga ttgattgatt tcagaggaca catcatgaat
     1861 tacgaggacg agttatccca acctgacctt gccgtcattg gtccaatggc tcgttcagca
     1921 cgggacttaa gtttattatt agatgtttta gtcgagccaa atgcggatga ctttagagct
     1981 tttaaactca aggaatctga aaaacaaacc attcaagagt ttaaagtatt attctggatg
     2041 gatgatgaat cgtgtccaat cgattcacgg ctaaagaaaa aatacgatca acttttgcaa
     2101 accctgcaaa ctgccaaggt taacgtggat gtaggtcgtc caaaaaattg ggattttgat
     2161 gaaatattta aatcctacgc cactcgattg atcagccaaa tgacttttgc agatcctaaa
     2221 ctaagtcgat tgtccatgtc ggcatctacg ccattaatca aattacttaa tggtaaagca
     2281 ggcattccgc ctttggccta tggatttaca caaggtgcta acctgagtca tgcagaatgg
     2341 cttgccaaat atgaagagtc attgcatatc aagcaaaaat gtcttgaaat ttttgcagaa
     2401 tacgacgtca ttatttgccc acctattttg actctagcgt ttgaacacaa tcataaagaa
     2461 cctttgatgt ttcgcacgct acaagtcgat ggaagaaaac gtttctatct tgagttattc
     2521 aaatggattt cgccagcaac tgtatttggg ttgccatcaa cctctgcacc gataggctta
     2581 tctgaggaca atctaccagt caatattcaa atattgggac aaccgtatgc agacaaagtc
     2641 accattaaat ttgctgaact gcttgcacaa attacggatg gattccaaaa accgcctttg
     2701 gatttttaat aa
//